Immunophenotypes in the Idiopathic Inflammatory Myopathies

项目摘要 候选人：Erin Wilfong博士，医学博士，博士是范德比尔特大学医学中心的临床讲师， 过敏，肺，重症监护医学和流变学与免疫学部门。她有一个强大的 具有风湿病学和肺病学的临床背景，在化学方面具有良好的科学基础 生物学和转化免疫学研究和博士后研究 经过临床培训。她的长期职业规划是成为炎症领域的领先医生-科学家， 肌炎集中在使用尖端技术，以确定疾病的发病机制和新的 治疗目标为了实现这一目标，她的直接目标是（1）学习必要的生物信息学技术， 独立分析高维度免疫表型和转录谱并整合这些 特征与临床结果，和（2）应用分子免疫学技术，以确定失调 IIM特异性细胞群中的途径。 研究项目：该提案将研究IIM的潜在免疫异质性，并确定 免疫/转录签名与临床特征之间的关联。Wilfong博士将（1）确定 使用横截面IIM队列研究IIM中的免疫特征和转录异常，以及（2）相关 纵向IIM队列中ILD进展的免疫特征。她也会先做单细胞RNA检测 Seq发现和（3）进行机制研究，研究氧化还原传感器TXNIP的上调如何改变 Jo 1+抗-tRNA合成酶综合征免疫代谢 职业发展：Wilfong博士的职业发展计划将正式课程与个性化课程相结合， 培训她的导师和合作者：（1）掌握R编程，以促进分析 免疫表型和转录数据集使用尖端的分析工具，（2）学习生物统计学 将免疫学发现与临床表型适当关联所需的原则，（3）表征 细胞功能使用体外分析，和（4）发展必要的领导和沟通技巧， 成为主要研究者，应用K 08奖项的科学发现和方法 走向未来的转化免疫学R 01。 环境：VUMC是促进Wilfong博士发展为翻译领域领导者的理想环境。 免疫学和炎性肌炎。Wilfong博士的指导团队包括炎症专家 肌炎（Crofford/Aggarwal）、免疫代谢（拉斯梅尔）、单细胞RNA-Seq（Kropski）和 计算免疫学（Georgiev）。除了拥有必要的导师和设备，71.3%的 范德比尔特大学医学中心职业发展奖获得者（n=236）已成功获得 自1999年以来，R级资助。克罗夫德博士和拉斯梅尔成功地培养了许多医学科学家 并将确保威尔方博士成为一名独立资助的医学科学家。

PROJECT SUMMARY Candidate: Dr. Erin Wilfong, M.D., Ph.D. is a Clinical Instructor at Vanderbilt University Medical Center in the Divisions of Allergy, Pulmonary, & Critical Care Medicine and Rheumatology & Immunology. She has a strong clinical background in both rheumatology and pulmonology with an excellent scientific foundation in chemical biology and translational immunology from both her Ph.D. studies and her post-doctoral research studies following clinical training. Her long-term career plan is to become a leading physician-scientist in inflammatory myositis focused on the use of cutting-edge technologies to identify disease pathogenesis and novel therapeutic targets. To achieve this, her immediate goal is to (1) learn the requisite bioinformatic techniques to independently analyze high-dimensional immunophenotypic and transcriptional profiles and integrate these profiles with clinical outcomes, and (2) apply molecular immunology techniques to determine dysregulated pathways in IIM-specific cell populations. Research Project: This proposal will study the underlying immunologic heterogeneity of IIM and identify associations between immune/transcriptional signatures and clinical characteristics. Dr. Wilfong will (1) identify immune signatures and transcriptional aberrancies in IIM using a cross-sectional IIM cohort and (2) correlate immune features with ILD progression in a longitudinal IIM cohort. She will also take a prior single-cell RNA- Seq finding and (3) perform mechanistic studies investigate how upregulation of the redox sensor TXNIP alters immunometabolism in Jo1+ anti-tRNA synthetase syndrome. Career Development: Dr. Wilfong’s career development plan integrates formal coursework with personalized training from her mentors and collaborators to: (1) master R programming to facilitate analysis of immunophenotypic and transcriptional datasets using cutting-edge analytic tools, (2) learn biostatistical principles necessary to appropriately correlate immunology findings to clinical phenotypes, (3) characterize cellular function using in vitro assays, and (4) develop the leadership and communication skills necessary to become a principal investigator who applies the scientific findings and methodologies from this K08 award towards a future translational immunology R01. Environment: VUMC is the ideal environment to foster Dr. Wilfong’s development as a leader in translational immunology and inflammatory myositis. Dr. Wilfong’s mentoring team includes experts in inflammatory myositis (Crofford/Aggarwal), immunometabolism (Rathmell), single-cell RNA-Seq (Kropski), and computational immunology (Georgiev). In addition to having the necessary mentors and equipment, 71.3% of Vanderbilt University Medical Center career development award recipients (n=236) have successfully received R level funding since 1999. Drs. Crofford and Rathmell have successfully trained many physician-scientists and will ensure that Dr. Wilfong becomes an independently funded physician scientist.