Botanical Core

植物核心

• 批准号: 10671058
• 负责人: Amar G. Chittiboyina
• 金额: $ 20.43万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10671058
• 关键词: Botanical; Core

BOTANICAL CORE: ABSTRACT/SUMMARY The overall purpose of the Botanical Core of the proposed University of Mississippi (UM) Botanical Dietary Supplements Research Center (BDSRC) is to ensure product integrity and advance chemistry research on the Spirulina-based product, Immulina™, using a combination of bioassay- and chemical-based approaches. Researchers affiliated with the proposed UM BDSRC have generated evidence that supports reproducible and mechanistically plausible effects of Immulina™ for enhancing host responses that may promote resilience against respiratory viral infections such as influenza which resulted in 959,000 patient hospitalizations and 79,400 deaths in United States alone. The Botanical Core will be headed by Amar Chittiboyina, PhD, a medicinal chemist with extensive research experience in natural product chemistry as well as standardization methodologies. The specific aims of the Botanical Core are to: 1. Ensure the unambiguous identification and develop additional safety testing on Arthrospira (Spirulina) platensis raw material that will be used in the production of sufficient quantities of Immulina™ for the proposed research projects; 2. Develop and validate a selective in vitro bioassay for quantitation of the toll-like receptor 2-dependent activity exhibited by the Braun-type lipoproteins in Immulina™; 3. Advance chemistry research on Immulina™ by establishing chemical-based authentication and standardization approaches, characterization of immune-inhibitory substances and detailed structural analysis of the active immune-enhancing Braun-type lipoproteins; and 4. Perform additional stability studies, provide sufficient quantities of well-characterized and safe ImmulinaTM for use in the proposed UM BDSRC research projects, and explore collaborative opportunities with other CARBON units. Expected outcomes include (1) achieving a higher level of product integrity and quality assurance of Immulina™ used in the proposed UM BDSRC projects; (2) design of an improved biological standardization method that will selectively detect only TLR2 agonists such as the Braun-type lipoproteins in Immulina™; (3) providing chemical-based authentication and standardization methods that will complement the bioassay-based standardization method and provide a robust system to fully characterize Immulina™ product material; and (4) to provide sufficient quantities of standardized Immulina™ working closely with other researchers from the UM BDSRC and collaborating with other units of CARBON on future efficacy studies of ImmulinaTM.

植物学核心：摘要/摘要 密西西比大学(UM)拟议的植物学核心的总体目的 补充剂研究中心(BDSRC)是确保产品完整性和推进化学研究的基础 螺旋藻为基础的产品，免疫™，使用生物测定和基于化学的方法相结合。 隶属于拟议的UM BDSRC的研究人员已经产生了支持可重复性和 免疫球藻™增强寄主反应的机制上可信的效果，这可能会促进弹性 预防流感等呼吸道病毒感染，导致959,000名患者住院和 仅在美国就有79,400人死亡。 植物学核心将由阿马尔·奇蒂博伊娜博士领导，他是一位拥有广泛研究的药物化学家 具有天然产物化学和标准化方法方面的经验。 植物学核心的具体目标是： 1.确保对节旋藻(Spirulina)的明确识别并开展额外的安全测试 将用于生产足够数量的免疫球藻™的钝顶藻原料 建议的研究项目； 2.建立和验证Toll样受体2依赖的选择性体外生物测定方法 免疫球藻™中布劳恩型脂蛋白的活性； 3.通过建立以化学为基础的鉴定和鉴定方法，促进金针菇™的化学研究 免疫抑制物质的标准化方法、表征和详细结构 分析主动免疫增强的布劳恩型脂蛋白；以及 4.进行额外的稳定性研究，提供足够数量的特性良好和安全的 在建议的UM BDSRC研究项目中使用ImmulinaTM，并探索合作 与其他碳单位合作的机会。 预期成果包括(1)实现更高水平的产品完整性和质量保证 金针菇™在拟建的UM BDSRC项目中的应用；(2)改进的生物标准化设计 只选择性地检测免疫球藻™中的布劳恩型脂蛋白等TLR2激动剂的方法；(3) 提供基于化学的认证和标准化方法，这些方法将补充基于生物测定的标准化方法，并提供一个强大的系统来充分表征免疫™产品材料； 和(4)与其他研究人员密切合作，提供足够数量的标准化免疫™ UM BDSRC并与其他碳单位合作进行ImmulinaTM的未来疗效研究。