Genomics Bioinformatics

项目摘要 核心B。基因组学生物信息学 提出了一个基因组学和生物信息学核心，以实现大规模并行测序的应用- 的方法，以促进对基因表达的病理程序的理解， 动脉粥样硬化和NASH的发展。本PPG所有四个项目的一个重要方面是 定义遗传、饮食和药理学干扰对全球基因工程的影响 在相关组织和细胞类型中表达。PPG基因组学和生物信息学核心将提供两个 提供补充服务，以推动这些努力;（1）提供技术支助和资源， 能够进行批量RNA-Seq、单细胞RNA-Seq、ATAC-Seq和ChIP-Seq测定的文库测序，以及2） 相应的生物信息学和计算支持，以允许对所得数据进行有效分析。

PROJECT SUMMARY Core B. Genomics Bioinformatics A Genomics and Bioinformatics Core is proposed to enable the application of massively parallel sequencing- based approaches to advance the understanding of pathological programs of gene expression that contribute to the development of atherosclerosis and NASH. An essential aspect of all four Projects of this PPG will be to define the consequences of genetic, dietary and pharmacologic perturbations on global programs of gene expression in relevant tissues and cell types. The PPG Genomics and Bioinformatics Core will provide two complementary services to advance these efforts; 1) technical support and resources for preparation and sequencing of libraries enabling bulk RNA-Seq, single cell RNA-Seq, ATAC-Seq and ChIP-Seq assays and 2) corresponding bioinformatics and computational support to allow effective analysis of the resulting data.