Genomics Bioinformatics

项目总结 核心B.基因组学生物信息学 基因组学和生物信息学核心被提出以实现大规模并行测序的应用-- 基于方法来促进对基因表达的病理程序的理解 动脉粥样硬化和NASH的发展。这个PPG的所有四个项目的一个基本方面是 定义遗传、饮食和药物扰动对全球基因程序的影响 在相关组织和细胞类型中的表达。PPG基因组学和生物信息学核心将提供两个 促进这些努力的补充服务；1)技术支持和资源，用于筹备和 能够进行散装RNA-Seq、单细胞RNA-Seq、ATAC-Seq和ChIP-Seq分析的文库测序和2) 相应的生物信息学和计算支持，以实现对结果数据的有效分析。

PROJECT SUMMARY Core B. Genomics Bioinformatics A Genomics and Bioinformatics Core is proposed to enable the application of massively parallel sequencing- based approaches to advance the understanding of pathological programs of gene expression that contribute to the development of atherosclerosis and NASH. An essential aspect of all four Projects of this PPG will be to define the consequences of genetic, dietary and pharmacologic perturbations on global programs of gene expression in relevant tissues and cell types. The PPG Genomics and Bioinformatics Core will provide two complementary services to advance these efforts; 1) technical support and resources for preparation and sequencing of libraries enabling bulk RNA-Seq, single cell RNA-Seq, ATAC-Seq and ChIP-Seq assays and 2) corresponding bioinformatics and computational support to allow effective analysis of the resulting data.