Core B: Shared Resource Core For Characterizing Antibody Responses To SARS-CoV-2 And Other Pathogenic Human Coronaviruses

核心 B：用于表征对 SARS-CoV-2 和其他致病性人类冠状病毒的抗体反应的共享资源核心

• 批准号: 10688373
• 负责人: Aravinda M. DeSilva
• 金额: $ 38.01万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-30 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10688373
• 关键词: 2019-nCoV; Affinity; Antibodies; Antibody Response; Antigen Receptors; Antigens; B-Lymphocytes; Biological Assay; Containment; Coronavirus; Coronavirus spike protein; Enzyme-Linked Immunosorbent Assay; Exposure to; Fc Receptor; Goals; Gold; Health care facility; Immune; Immune Sera; Immunoglobulin Genes; Individual; Infection; Label; Length; Mammalian Cell; Measures; Methods; Middle East Respiratory Syndrome; N-terminal; North Carolina; Nucleocapsid; Paralysed; Pathogenicity; Pattern; Persons; Production; Property; Proteins; Recombinants; Research; Research Project Grants; Resource Sharing; SARS coronavirus; Science; Serology; Serology test; Serum; Site; Sorting - Cell Movement; Specificity; Structure; System; Testing; Vaccines; Viral; Viral Pathogenesis; Virus; base; detection assay; human coronavirus; milligram; pandemic disease; receptor binding

Abstract High-quality recombinant antigens and reliable and accurate SARS-CoV-2 serological assays are urgently needed for surveillance and for defining the immune mechanisms of viral pathogenesis, viral clearance, and protection against reinfection. Core B of the UNC SARS-CoV-2 Serological Sciences Center of Excellence will apply state-of-the-art strategies to establish methods for the production of milligram quantities of homogeneous recombinant spike protein CoV antigens with versatile tags and site-specific labels and use these antigens to develop sensitive and reliable singleplex and multiplex Ab detection assays. Core B will interact and support all the projects driven by the center and collaborators' by providing recombinant antigens and serological assays to characterize the serum antibody response, elucidate Fc effector functional profiles, and isolate and sequence antigen-specific immunoglobulin gene repertoire.

摘要 因此，高质量的重组抗原和可靠、准确的SARS-CoV-2血清学检测方法是当务之急 需要监测和定义病毒发病机制的免疫机制，病毒清除， 防止再感染。SARS-CoV-2血清学科学卓越中心的核心B将 应用最先进的战略，建立生产毫克量的均匀的方法， 具有通用标签和位点特异性标记的重组刺突蛋白CoV抗原，并使用这些抗原 开发灵敏可靠的单重和多重抗体检测分析。核心B将与所有 由中心和合作者推动的项目，通过提供重组抗原和血清学检测， 表征血清抗体应答，阐明Fc效应子功能谱，并分离和测序 抗原特异性免疫球蛋白基因库。