Single-molecule approaches to study epiblast stem cell fate decision

研究外胚层干细胞命运决定的单分子方法

• 批准号: 10690884
• 负责人: Farhan H Chowdhury
• 金额: $ 9.99万
• 依托单位: SOUTHERN ILLINOIS UNIVERSITY CARBONDALE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10690884
• 关键词: 3-Dimensional; Award; Binding; Biological Assay; Cell Communication; Cell Fate Control; Cells; Complex; Engineering; Epiblast; Equipment; Funding; Goals; Grant; Knowledge; Molecular; Regenerative Medicine; Research; Science; System; Time; Tissue Engineering; career; cell type; graduate student; imager; single molecule; stem cell fate; stem cells; training opportunity; undergraduate student

PROJECT SUMMARY An equipment supplement award for 1R15GM140448-01 (Title: Single-molecule approaches to study epiblast stem cell fate decision) is requested to acquire Leica’s THUNDER imager live cell and 3D assay system. Exploiting the advances in the computational clearing feature in real-time, this equipment will not only accelerate our experimental endeavors but also offer training opportunities for undergraduate and graduate students thus opening exciting new career opportunities for them in the biomedical science and engineering field. The main goal of the funded R15 grant is to elucidate the molecular force-dependent fate decision of epiblast stem cells. This equipment supplement award, if granted, will undoubtedly elevate our research and serve as the primary workhorse for carrying out the funded R15 project.

项目摘要 1 R15 GM 140448 -01的设备补充奖（标题：单分子方法， 研究上胚层干细胞命运决定），以获取Leica的THUNDER成像仪活细胞 和3D分析系统。利用实时计算清算功能的进步， 这些设备不仅可以加速我们的实验工作， 为本科生和研究生提供机会，从而开启令人兴奋的新职业生涯 为他们在生物医学科学和工程领域提供机会。的主要目标 R15基金是为了阐明外胚层干细胞的分子力依赖的命运决定， 细胞这项设备补充奖，如果授予，无疑将提高我们的研究， 作为执行受资助的R15项目的主要工具。

项目成果

Reduced Cell-ECM Interactions in the EpiSC Colony Center Cause Heterogeneous Differentiation.

• DOI: 10.3390/cells12020326
• 发表时间: 2023-01-15
• 期刊: Cells
• 影响因子: 6