Development of a Portable Ion Mobility Spectrometer For Efficient Diagnosis of Various Diseases

开发便携式离子淌度光谱仪以有效诊断各种疾病

• 批准号: 10708021
• 负责人: Ahmed Mohamed Hamid
• 金额: $ 37.41万
• 依托单位: AUBURN UNIVERSITY AT AUBURN
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-22 至 2027-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10708021
• 关键词: Alzheimer' s Disease; Antibiotic Resistance; Biological Markers; Clinical; Communities; Complement; Complex; Detection; Development; Devices; Diagnosis; Diagnostic Equipment; Diagnostic tests; Disease; Hospitalization; Ions; Isomerism; Lead; Methods; Molecular Structure; Nurses; Patient Care; Persons; Play; Preparation; Recovery; Research; Resolution; Resource-limited setting; Role; Sampling; Structure; Technology; Travel; Tube; Validation; Variant; advanced analytics; biomarker identification; clinical care; conformer; diagnostic biomarker; foodborne; high throughput analysis; innovation; interest; ion mobility; ionization; mobility aid; new technology; novel strategies; point-of-care diagnostics; portability; rapid diagnosis; skills; targeted biomarker; usability

Development of a Portable Ion Mobility Spectrometer for Efficient Diagnosis of various diseases This research will focus on the identification of the biomarkers associated with various diseases and the development and validation of an innovative high-resolution ion mobility (IM) device. The proposed device is based on a new variant of Structures for Lossless Ion Manipulations (SLIM) and will couple high-resolution ion mobility separations achieved by traveling wave (TW)-SLIM with drift tube (DT)-SLIM which is a novel approach that can provide collision cross section (CCS) values of the separated isomers/conformers that are unique to the target biomarkers of interest. We expect this capability to increase biomarkers’ identification accuracy. In addition, by combining the attributes of the high-resolution ion mobility and ambient ionization, sensitive detection, and superior separation power will be achieved in the analysis of complex clinical samples without any prior sample preparation. The current diagnosis methods are often slow, expensive, or laborious which limits their use in resource-limited regions. Therefore, there is a strong unmet need for new technologies that can rapidly diagnose various diseases to complement the existing commercially available technologies. Rapid identification of diagnostic biomarkers in clinical samples will not only lead to an increase in recovery rates but also will lead to shorter hospitalization stays and reduced antibiotic resistance. We aim to develop a portable IM diagnostic device that can be used for the identification of the biomarkers associated with foodborne and Alzheimer’s diseases which significantly impact the lives of millions of people. The significance of these diseases causes diagnostic tests to play a major role in the clinical care of patients suffering from these diseases Our studies will enable high throughput analyses that are affordable and so easy-to-operate that can be performed by less-skilled users, such as nurses and their assistants. Over the long term, these studies will be extended to multiple applications that span from the fundamental studies of molecular structure elucidation to advanced analytical applications such as a widely usable point-of-care diagnostic device that can serve the community, especially resource-limited regions. 1

便携式离子迁移谱仪的研制 本研究将集中于鉴定与各种疾病相关的生物标志物， 创新的高分辨率离子迁移率（IM）设备的开发和验证。申报器械 基于无损离子操纵结构（SLIM）的新变体， 采用行波SLIM和漂移管SLIM实现了迁移率分离 其可以提供对所述异构体/构象异构体唯一的分离的异构体/构象异构体的碰撞截面（CCS）值。 目标生物标志物。我们希望这种能力能够提高生物标志物识别的准确性。在 此外，通过结合高分辨率离子迁移率和环境电离的属性， 检测，并且在分析复杂的临床样品时将实现上级分离能力， 任何先前的样品制备。 目前的诊断方法往往是缓慢的，昂贵的，或费力的，这限制了它们在资源有限的情况下的使用。 地区因此，对能够快速诊断各种疾病的新技术存在强烈的未满足需求 以补充现有的商用技术。快速鉴定诊断性生物标志物 临床样本不仅可以提高治愈率，还可以缩短住院时间 减少抗生素耐药性。我们的目标是开发一种便携式IM诊断设备，可用于 确定与食源性疾病和阿尔茨海默病相关的生物标志物， 影响着数百万人的生活。这些疾病的重要性导致诊断测试发挥主要作用 我们的研究将使高通量分析成为可能 其是负担得起的并且易于操作的，其可以由不太熟练的用户（诸如护士及其护理人员）来执行。 助手从长远来看，这些研究将扩展到从 分子结构解析的基础研究，以先进的分析应用，如广泛的 可用的床旁诊断设备，可以服务于社区，特别是资源有限的地区。 1