Human Tissue Procurement and Processing Core

人类背根神经节(DRG)和脊髓的工作已经通过协作进行了转变 与器官移植康复机构的合作。在过去的几年里，我们开发了一种可扩展的 与器官移植机构合作，回收60多套背根节和脊髓 来自器官捐赠者。这种合作导致了几个共同撰写的出版物和新的 方法从参与该项目的医院的外科病人身上回收额外的神经系统组织。 疼痛领域的一个关键问题是人类伤害性感受器是否会改变其分子组成 出现持续性或慢性疼痛。我们将利用这个核心的独特资源来直接回答这个问题， 并生成数据集，以回答疼痛治疗发现领域的新问题。我们的首要目标是建立 关于我们与器官捐赠者的现有工作，以制定组织恢复的行政和科学方案。一把钥匙 里程碑将是每年从50名器官捐赠者中恢复背根节、脊髓和周围神经。在我们的 第二个目标，我们将扩大我们对疼痛患者DRG的独特访问，这些患者在相对较短的时间内恢复 罕见的手术。在全国范围内促进更多的这类研究，并加强对这些研究的获取 对于有价值的组织，我们将创建IRB模板、符合医院标准操作程序(SOP)和 其他关键的行政和科学方法，以促进这类研究的扩散。最后，在我们的 第三个目标，我们将利用我们在RNAScope技术方面的丰富经验来比较一组 细胞表达不同的标记物(低、中、高表达)，以准确了解组织如何 转录本可能会根据捐赠者的时间线和其他因素而变化。这将使现场能够 了解如何在不同的研究类型之间进行比较。这个核心将创造一个庞大的组织资源， 技术方法和管理熟练程度，将使该领域的重要发现，以及 将人类神经系统组织用于基础疼痛研究的范围远远超出了 该中心的合作实验室。

Work with human dorsal root ganglia (DRG) and spinal cords has been transformed by collaborative efforts with organ transplant recovery agencies. Over the past years, we have developed an expansive collaboration with organ transplant agencies enabling recovery of more than 60 sets of DRGs and spinal cords from organ donors. This collaboration has led to several co-authored publications and the development of new methods to recover additional nervous system tissues from surgical patients at hospitals involved in this project. A key question in the pain field is whether human nociceptors change their molecular makeup when a patient develops persistent or chronic pain. We will use the unique resources of this core to directly answer this question, and generate datasets to answer new questions for the pain therapeutic discovery field. Our first aim will build on our existing work with organ donors to create administrative and scientific protocols for tissue recovery. A key milestone will be the recovery of DRG, spinal cord and peripheral nerves from 50 organ donors per year. In our second aim we will expand our unique access to DRGs from pain patients that are recovered during relatively rare surgeries. To facilitate more of this type of research throughout the country, and enhance access to these valuable tissues, we will create IRB templates, hospital compliant standard operating procedures (SOPs) and other key administrative and scientific methods to enable the proliferation of this type of research. Finally, in our third aim we will leverage our deep experience with RNAscope technology to compare expression of a group of markers that vary in cellular expression (low, mid, and high expression) to understand exactly how tissue transcriptomes might change depending on donor timeline and other factors. This will allow the field to understand how to make comparisons across study types. This core will create an expansive resource of tissue, technical methods and administrative proficiency that will enable important discoveries for the field, and the expansion of the use of human nervous system tissues for basic pain research far beyond the walls of the collaborating laboratories in this Center.