Human Tissue Procurement and Processing Core

人体组织采购和加工核心

• 批准号: 10707550
• 负责人: Stephanie Shiers
• 金额: $ 51.88万
• 依托单位: UNIVERSITY OF TEXAS DALLAS
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-19 至 2027-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10707550
• 关键词: Autopsy; Award; Biological Assay; Chest; Chronic disabling pain; Collaborations; Country; Data Set; Data Sources; Development; Dissociation; Funding; Goals; Hospitals; Human; Institutional Review Boards; Intervention Studies; Knowledge; Laboratories; Low Back Pain; Lumbar nerve; Maps; Methods; Molecular; Molecular Profiling; Nervous System; Neurons; Nociceptors; Nodose Ganglion; Operative Surgical Procedures; Organ Donations; Organ Donor; Organ Transplantation; Pain; Pain Research; Patients; Peripheral Nerves; Persistent pain; Phenotype; Physicians; Price; Procedures; Proliferating; Protocols documentation; Publications; Quality Control; RNA; Recording of previous events; Recovery; Research; Research Design; Resected; Resources; Sampling; Site; Slice; Spinal Cord; Spinal Ganglia; Technology; Therapeutic; Tissue Procurements; Tissues; Transplantation; United States; United States National Institutes of Health; University of Texas M D Anderson Cancer Center; Work; arthropathies; chronic pain; experience; human tissue; pain patient; timeline; tissue processing; tissue resource; transcriptome; transcriptomics

Work with human dorsal root ganglia (DRG) and spinal cords has been transformed by collaborative efforts with organ transplant recovery agencies. Over the past years, we have developed an expansive collaboration with organ transplant agencies enabling recovery of more than 60 sets of DRGs and spinal cords from organ donors. This collaboration has led to several co-authored publications and the development of new methods to recover additional nervous system tissues from surgical patients at hospitals involved in this project. A key question in the pain field is whether human nociceptors change their molecular makeup when a patient develops persistent or chronic pain. We will use the unique resources of this core to directly answer this question, and generate datasets to answer new questions for the pain therapeutic discovery field. Our first aim will build on our existing work with organ donors to create administrative and scientific protocols for tissue recovery. A key milestone will be the recovery of DRG, spinal cord and peripheral nerves from 50 organ donors per year. In our second aim we will expand our unique access to DRGs from pain patients that are recovered during relatively rare surgeries. To facilitate more of this type of research throughout the country, and enhance access to these valuable tissues, we will create IRB templates, hospital compliant standard operating procedures (SOPs) and other key administrative and scientific methods to enable the proliferation of this type of research. Finally, in our third aim we will leverage our deep experience with RNAscope technology to compare expression of a group of markers that vary in cellular expression (low, mid, and high expression) to understand exactly how tissue transcriptomes might change depending on donor timeline and other factors. This will allow the field to understand how to make comparisons across study types. This core will create an expansive resource of tissue, technical methods and administrative proficiency that will enable important discoveries for the field, and the expansion of the use of human nervous system tissues for basic pain research far beyond the walls of the collaborating laboratories in this Center.

与人类背根神经节（DRG）和脊髓的合作已经转变为 与器官移植恢复机构合作。在过去的几年里，我们已经开发了一个广阔的 与器官移植机构合作，恢复60多套DRG和脊髓 器官捐赠者。这种合作导致了几个共同撰写的出版物和新的开发 从参与该项目的医院的手术患者中恢复额外神经系统组织的方法。 疼痛领域的一个关键问题是，当患者受到刺激时， 持续或慢性疼痛。我们将利用这个核心的独特资源来直接回答这个问题， 并生成数据集来回答疼痛治疗发现领域的新问题。我们的第一个目标是 我们与器官捐赠者的现有工作，以创建组织恢复的行政和科学协议。一个关键 里程碑将是每年从50名器官捐献者中恢复DRG、脊髓和外周神经。在我们 第二个目标，我们将扩大我们独特的获得DRG的疼痛患者， 罕见的手术为了在全国范围内促进更多的这类研究，并增加获得这些研究的机会， 对于有价值的组织，我们将创建IRB模板、医院合规的标准操作规程（SOP）， 其他关键的行政和科学方法，使这类研究的扩散。最后，在我们的 第三个目标，我们将利用我们在RNAscope技术方面的丰富经验，比较一组 细胞表达不同的标记物（低，中和高表达），以准确了解组织 转录组可能根据供体时间轴和其他因素而改变。这将允许字段 了解如何进行跨研究类型的比较。这个核心将创造一个广阔的组织资源， 技术方法和管理能力，这将使该领域的重要发现， 将人类神经系统组织用于基础疼痛研究的扩展远远超出了 实验室在这个中心。