Spinning Disk Super Resolution Confocal Microscope for the Vanderbilt Brain Institute

范德比尔特脑研究所的转盘超分辨率共焦显微镜

• 批准号: 10721222
• 负责人: Jenny Schafer
• 金额: $ 59.78万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10721222
• 关键词: 3-Dimensional; Academic Medical Centers; Aggressive behavior; Area; Attention deficit hyperactivity disorder; Award; Basic Science; Brain; Cells; Communication; Computers; Core Facility; Dedications; Equipment; Faculty; Fees; Financial Support; Funding; Image; Impulsivity; Incubated; Institution; Interdisciplinary Study; Investigation; Lasers; Learning; Medical Research; Memory; Mental Health; Microscope; Microscopy; Minor; Molecular; National Institute of Mental Health; Neurosciences; Organoids; Population; Recording of previous events; Research; Research Institute; Research Personnel; Resolution; Resource Sharing; Rett Syndrome; Sampling; Signal Transduction; Social Interaction; Specialist; Speed; Synapses; System; Tissues; Training; United States National Institutes of Health; Universities; Wages; cellular imaging; confocal imaging; instrument; lens; live cell imaging; microscopic imaging; new technology; operation; superresolution imaging; synaptogenesis; ultra high resolution

PROJECT SUMMARY / ABSTRACT Funds are requested to purchase a Nikon Spinning Disk Super Resolution (CSU-W1/SoRa) Confocal microscope for imaging projects focused around mental health research within the Vanderbilt Brain Institute (VBI), a multi-disciplinary research institute comprised of faculty at Vanderbilt University (VU) and Vanderbilt University Medical Center (VUMC). The requested instrument will be integrated into and managed by the Vanderbilt Cell Imaging Shared Resource (CISR), a microscopy core with a 20+ year history of successful microscopy management, training, and acquisition of new technology. This instrument capable of confocal imaging and super resolution imaging in live samples is ideal for 6 Major and 7 Minor Users within the VBI with projects surrounding synaptogenesis, synapse assembly and plasticity, subsynaptic signaling, and communication between cell and tissue populations. These molecular and cellular investigations will advance already funded NIMH and NIH projects totaling over $6.8M in FY22 in the areas of social interaction, impulsive aggression, learning and memory, Rett syndrome (RTT), ADHD, and more. The proposed microscope configuration is based on the needs of the VBI and Major Users for live imaging at cellular and subcellular resolution. The Nikon CSU-W1 SoRa confocal microscope includes: 1) CSU-W1 spinning disk plus Yokogawa SoRa super resolution spinning disk for super resolution near 120 nm in XY; 2) stage-top incubation for imaging live cells and organoids; 3) Z-Piezo stage for increased speed in Z for 3D volumes; 4) Hamamatsu Orca-Fusion sCMOS camera with 30-100 frames per second; 5) excitation lasers of 405, 488, 561, and 633 nm; and 6) objective lenses for live cell imaging at high resolution ranging from 10x, 20x, 40x, 60x, and 100x. This new system would be housed in existing CISR space in Medical Research Building III (MRBIII) where the VBI is based and the majority of the Major User labs are located. Vanderbilt University is providing financial support to promote the long-term operation of the instrument. If awarded, the Office of the Dean of Basic Sciences is committed to providing $75,000 for CISR staff salary, computer purchases and upgrades, and reduction in user fees over the first 5 years. Technical support and training of new users will be facilitated by the exceptional staff within the CISR, which has a greater than 20-year history of assisting over 300 labs on campus. Moreover, this equipment will become part of the Vanderbilt Nikon Center of Excellence (COE) that is housed within the CISR. This CISR/Nikon COE provides a dedicated local team of specialists to support the microscopy needs of investigators on campus. Collectively, the proposed confocal system, together with outstanding institutional and core facility support, will enable the long-term objective of supporting and advancing neuroscience and mental health research Vanderbilt.

项目总结/摘要 Nikon Spinning Disk Super Resolution（CSU-W1/索拉）Confocal 范德比尔特脑科学研究所（Vanderbilt Brain Institute）的一台显微镜，用于围绕心理健康研究的成像项目 (VBI)这是一个多学科研究机构，由范德比尔特大学（VU）和范德比尔特大学的教师组成 大学医学中心（VUMC）。所要求的文书将纳入联合国系统，并由联合国系统管理。 范德比尔特细胞成像共享资源（CISR），一个具有20多年成功历史的显微镜核心， 显微镜管理、培训和获取新技术。该仪器能够共焦 实时样品成像和超分辨率成像是VBI内6个主要用户和7个次要用户的理想选择， 项目围绕突触发生，突触组装和可塑性，突触下信号， 细胞和组织群体之间的通讯。这些分子和细胞的研究将推动 已经资助了NIMH和NIH的项目，在2022财年在社会互动，冲动， 攻击，学习和记忆，Rett综合征（RTT），ADHD等等。 建议的显微镜配置是基于VBI和主要用户的实时成像需求， 细胞和亚细胞分辨率。尼康CSU-W1索拉共聚焦显微镜包括：1）CSU-W1 旋转盘+Yokogawa索拉超分辨率旋转盘，XY方向超分辨率接近120 nm; 2） 用于活细胞和类器官成像的载物台顶部孵育; 3）Z-压电载物台，用于提高3D Z轴速度 体积; 4）Hamamatsu Orca-Fusion sCMOS相机，每秒30-100帧; 5） 405、488、561和633 nm;以及6）用于以10倍，20倍， 40 x、60 x和100 x。这一新系统将安置在医学研究大楼III的现有CISR空间内 （MRBIII），VBI所在地和大多数主要用户实验室所在地。 范德比尔特大学正在提供财政支持，以促进该仪器的长期运行。如果 授予，基础科学院长办公室致力于为CISR工作人员工资提供75，000美元， 计算机购买和升级，并在头5年减少用户费用。技术支持和 新用户的培训将由CISR内部的优秀工作人员提供便利，CISR拥有超过20年的 协助校园内300多个实验室的历史。而且，这款设备将成为范德比尔特尼康的一部分 卓越中心（COE）位于CISR内。此CISR/Nikon COE提供专用的本地 专家团队，以支持校园内研究人员的显微镜需求。总体而言，拟议的 共聚焦系统，加上出色的机构和核心设施支持，将使长期 支持和推进神经科学和心理健康研究的目标范德比尔特。