Morphology and Phenotyping Core

形态学和表型核心

• 批准号: 7677654
• 负责人: Maranke I. Koster
• 金额: $ 14.68万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-28 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7677654
• 关键词: Affect; Antibodies; Consultations; Custom; Differentiation Antigens; Disease; Equipment; Gene Expression Profiling; Genes; Harvest; Histology; Human; Human Resources; In Situ Hybridization; Laboratory Research; Modeling; Molecular Analysis; Morphology; Pathology; Pattern; Phenotype; Preparation; Process; Quantitative Evaluations; Research Personnel; Sampling; Services; Skin; Staining method; Stains; Structure; Tissues; Training; Transcript; appendage; experience; interest; member; model design; mouse model; skin disorder; tissue processing; tool

One of the most basic needs of investigators studying skin disease is the ability to use tissue sections to analyze the pathological changes at the tissue and cellular level. This analysis requires access to specialized equipment for tissue processing and to highly trained personnel, neither of which can be afforded by most research laboratories. Furthermore, since qualitative and quantitative evaluation of skin and skin appendages requires precise and reproducible orientation of skin sections, histology services not specialized in skin generally produce skin sections of substandard quality. Therefore, the objective of the Morphology and Phenotyping Core is to provide investigators with the tools and expertise to process and analyze skin samples. The Core owns the equipment necessary to process and section skin samples and employs a histology technician trained in processing skin samples. Thus, the Core is able to provide investigators with high quality processing and sectioning of skin samples as well as with the preparation of basic histological stains. Whereas histological analysis of skin samples represents the first step in analyzing skin phenotypes, subsequent analyses include immunostaining for various markers of skin proliferation and differentiation. The Core possesses a wide variety of custom-made antibodies that recognize such markers and will provide investigators with immunostaining services using these antibodies. The Core will also offer in situ hybridization services to allow investigators to determine transcript expression patterns of their gene of interest. Finally, the Core will offer consultation services including assistance with harvesting skin samples, analyzing histological stains, analyzing antibody staining results, and analyzing in situ hybridization results.

研究皮肤病的研究人员最基本的需求之一是能够使用组织切片， 从组织和细胞水平分析病理变化。此分析需要访问 用于组织处理的专门设备和训练有素的人员，这两者都负担不起 大多数研究实验室。此外，由于对皮肤和皮肤的定性和定量评价， 附件需要精确和可重复的皮肤切片方向，组织学服务不专业 在皮肤中通常产生质量不合格皮肤部分。因此，形态学的目标 和表型核心是为研究人员提供工具和专业知识，以处理和分析皮肤 样品核心拥有必要的设备，处理和切片皮肤样本，并聘请了 接受过处理皮肤样本培训的组织学技术员。因此，核心小组能够向调查人员提供 高质量的皮肤样本处理和切片以及基础组织学的准备 渍.尽管皮肤样本的组织学分析代表了分析皮肤表型的第一步， 随后的分析包括对皮肤增殖和分化的各种标记进行免疫染色。 核心拥有各种各样的定制抗体，可以识别这些标记， 使用这些抗体的免疫染色服务的研究人员。核心还将提供现场 杂交服务，使研究人员能够确定其基因的转录表达模式， 兴趣最后，核心将提供咨询服务，包括协助采集皮肤样本， 组织学染色分析、抗体染色结果分析和原位杂交结果分析。