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Molecular Biology-Mouse Core

Molecular Biology-Mouse Core
分子生物学-小鼠核心
批准号：
7760728
负责人：
Josef Anrather
金额：
$25.94万
依托单位：
WEILL MEDICAL COLL OF CORNELL UNIV
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
项目状态：
已结题
起止时间：
2009-08-05 至 2014-06-30
来源：
https://reporter.nih.gov/project-details/7760728
关键词：
Antibody SpecificityBiochemicalBrainCell LineGene TargetingGenerationsHypertensionMolecularMolecular BiologyMusNADPH OxidaseProductionProsencephalonReactive Oxygen SpeciesServicesSpecificityTestingTissue HarvestingTransfectionViral Genesanimal tissuecDNA Expressiontissue culture

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2). 5. Generation of tagged cDNA expression constructs for testing antibody specificity (all Projects). 6. Provision of viral gene targeting constructs (Proiects 1. 2, and 4). II. Tissue culture services 1. Culture and transfection of cell lines to test immunoreagent specificity (all Projects). 2. Regional tissue harvesting for molecular and biochemical analysis (Proiects 1. 2. and 4). 3. Isolation of brain microvessels (Proiect 4). III. Biochemical services 1. Analysis of reactive oxygen species (ROS) production in animal tissue (Proiects 1. 3. and 4). 2. Determination of NADPH Oxidase activity in animal tissue (
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