Tissue imaging, biochemistry, and morphology

组织成像、生物化学和形态学

• 批准号: 7613219
• 负责人: MICHAEL C HOGAN
• 金额: $ 41.02万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-08 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7613219
• 关键词: Accreditation; American; Animal Experimentation; Animals; Apple; Basic Science; Biochemistry; Blood Vessels; Caring; Chronic Obstructive Airway Disease; Clinical; Color; Computers; Educational process of instructing; Electron Microscope; Equipment; Exercise; Glass; Health; High Pressure Liquid Chromatography; Holidays; Hospitals; Hour; Image; Institutes; Laboratories; Laboratory Animals; Light Microscope; Location; Lung; Medical; Microfilm; Microfilming; Microscope; Microtome - medical device; Microtomy; Modeling; Morphology; NADH; Oceanography; Oxygen; Perfusion; Physiology; Plastics; Process; Reader; Resources; Series; Services; Spottings; Supervision; System; Techniques; Tissues; United States Department of Agriculture; United States Department of Veterans Affairs; United States Public Health Service; Universities; Work; animal care; animal facility; base; cellular imaging; computer center; fluorescence microscope; instrument; pressure; square foot; supercomputer

in the Medical Teaching Facility adjacent to the Basic Science Building at UCSD. A laboratory of 480 square feet is available for experimental work, and two other small laboratories of 240 square feet each are available for EM processing, thin sectioning and photographic work. Clinical: "- NA Animal: UCSD is fully accredited by the American Association for Accreditation of Laboratory Animal Care. There are currently over 92,000 sq. ft. of animal facilities at 24 locations. Veterinary care for all University research animals is under the supervision of the Campus Office of Veterinary Services on a twenty-four hour basis, including holidays and weekends, in accordance with the Public Health Service, U.S. Department of Agriculture and American Association for Accreditation of Laboratory Animal care requirements. Computer: Excellent computer facilities in our laboratories, including Pentium III and IV PCs and Apple computers, access by hard line to the campus computer center, and access to the San Diego Supercomputer which is located on campus are available if necessary. Office: Dr. Michael Hogan has exclusive use of an office in the Medical Teaching Facility, across the hall from the laboratories. Other: Electron Microscopes are available on campus at the Veterans Administration Hospital and UCSD Scripps Institute of Oceanography. They are used on a recharge basis. We have used these microscopes over the past 20 years and they are satisfactory for our purposes. MAJOR EQUIPMENT: List the most important equipment items already available for this project, noting the location and pertinent capabilitiesof each. Permanent laboratory equipment relevant to this Core in the Division of Pulmonary Physiology includes three LKB Ultrotome III (thin sectioning for EM), LKB and RMC glass knife breakers, Jung Biocut 2035 microtome, Reichert Jung Cryocut 1800, Meiji EMZ Series dissecting microscope for trimming plastic EM blocks, Leitz Ortholux and Laborlux light microscopes equipped with Leica cameras, Jenalumar fluorescence microscope equipped with SPOT RT 215-3 camera, American Innovision and Image Pro Plus 4.5.1. image analyzers, Sony DXC 960MD color video camera, Sony UP-5600 MD color video printer, Jena and Bell Howel microfilm readers, Osmometer (Model 3W, Advanced Instruments), fume hood, darkroom, and Revco freezer -80 ¿C. Equipment for vascular perfusion and pressure recording are also available. For cellular imaging, two PTI fluorescent systems, a McBain Confocal Microscope system, and a system for phosphorecent imaging of intracellular PO2 (Oxygen Systems) are available for all Units. Wet biochemistry using standard NADH fluorescent techniques along with a HPLC are also within these labs. PHS 398/2590 (Rev. 04/06) Page 270 Resources Format Page

在 毗邻UCSD基础科学大楼的医学教学设施。实验室480平方米 有两个240平方英尺的小实验室， 可用于EM处理、薄切片和摄影工作。 临床：“- NA 动物： UCSD获得了美国实验动物护理认证协会的全面认证。那里 目前已超过92，000平方米。英尺24个地点的动物设施为所有大学研究提供兽医护理 动物在兽医服务的校园办公室的监督下，24小时， 包括节假日和周末，根据美国公共卫生署， 农业和美国实验动物护理认证协会的要求。 电脑： 我们实验室的电脑设备非常完善，包括奔腾III和IV个人电脑以及苹果电脑， 通过硬线访问校园计算机中心，并访问圣地亚哥超级计算机， 在校园里，如果有必要的话。 办事处： 博士迈克尔霍根在医学教学设施的办公室独家使用，从大厅对面 laboratories. 其他： 电子显微镜可在校园退伍军人管理医院和UCSD斯克里普斯 海洋研究所。它们在充电的基础上使用。我们用这些显微镜 过去20年来，他们对我们的目的感到满意。 主要设备：列出本项目已可用的最重要的设备项目，并注明每个设备的位置和相关功能。 肺生理学分部中与本核心相关的永久性实验室设备包括三个 LKB Ultrotome III（EM薄切片），LKB和RMC玻璃刀破碎机，Jung Biocut 2035切片机， Reichert Jung Cryocut 1800，Meiji EMZ系列解剖显微镜，用于修剪塑料EM块，Leitz 配备Leica摄像头的Ortholux和Rollux光学显微镜，Jenalumar荧光显微镜 配备SPOT RT 215-3相机，美国Innovision和Image Pro Plus 4.5.1。图像分析仪， Sony DXC 960 MD彩色摄像机、Sony UP-5600 MD彩色视频打印机、Jena和Bell Howel缩微胶卷 读数器、渗透压计（型号3 W，Advanced Instruments）、通风橱、暗室和Revco冷冻箱-80 ℃。 还提供血管灌注和压力记录设备。对于细胞成像，两个PTI 荧光系统、McBain共聚焦显微镜系统和用于磷光体成像的系统。 细胞内PO 2（氧气系统）可用于所有单位。使用标准NADH的湿生物化学 荧光技术沿着高效液相色谱法也在这些实验室中。 PHS 398/2590（Rev. 04/06）