Tissue imaging, biochemistry, and morphology

在……里面 加州大学圣迭戈分校基础科学大楼旁边的医学教学设施。占地480平方米的实验室 英尺可用于实验工作，另外两个分别为240平方英尺的小型实验室 可用于EM加工、薄片切割和摄影工作。 临床部：“- 北美 动物： 加州大学圣迭戈分校获得美国实验动物护理认可协会的完全认可。那里 目前超过92,000平方英尺。英国《金融时报》24个地点的动物设施。为所有大学研究提供兽医关怀 动物由校园兽医服务办公室24小时监督， 包括节假日和周末，根据美国公共卫生服务，美国 农业和美国实验动物护理要求认可协会。 计算机： 我们实验室拥有一流的计算机设施，包括奔腾III和奔腾IV PC以及苹果电脑， 通过硬线访问校园计算机中心，并访问圣地亚哥超级计算机 如有必要，可在校园内使用。 办公室： 迈克尔·霍根博士在医学教学设施的一间办公室是独家使用的，就在大厅对面 实验室。 其他： 退伍军人管理医院和加州大学斯克里普斯分校的校园里都有电子显微镜 海洋研究所。它们是在充电的基础上使用的。我们已经用过这些显微镜了 过去20年，它们对我们的目的是令人满意的。 主要设备：列出本项目已有的最重要的设备，注明每个设备的位置和相关能力。 肺生理科与该核心有关的常设实验室设备包括三个 LKB Ultrotome III(用于EM的薄片)，LKB和RMC玻璃刀破碎机，Jung Biocut 2035切片机， Reichert Jung Cryocut 1800，Meiji EMZ系列切割塑料EM块的解剖显微镜，莱茨 配备徕卡相机的Ortholux和Lablux光学显微镜，Jenalar荧光显微镜 配备Spot RT 215-3摄像头、American Innovision和Image Pro Plus 4.5.1。图像分析仪， 索尼DXC 960MD彩色摄像机，索尼UP-5600 MD彩色视频打印机，Jena和Bell Howel缩微胶片 阅读器、渗透率计(3W型，先进仪器)、通风罩、暗室和Revco冷冻机-80C。 还提供血管灌流和压力记录设备。对于细胞成像，两个PTI 荧光系统、麦克贝恩共聚焦显微镜系统和用于磷光最近成像的系统 细胞内PO2(氧气系统)适用于所有单元。使用标准NADH的湿生物化学 荧光技术和高效液相色谱仪也在这些实验室中。 PHS 398/2590(04/06版)第270页资源格式页

in the Medical Teaching Facility adjacent to the Basic Science Building at UCSD. A laboratory of 480 square feet is available for experimental work, and two other small laboratories of 240 square feet each are available for EM processing, thin sectioning and photographic work. Clinical: "- NA Animal: UCSD is fully accredited by the American Association for Accreditation of Laboratory Animal Care. There are currently over 92,000 sq. ft. of animal facilities at 24 locations. Veterinary care for all University research animals is under the supervision of the Campus Office of Veterinary Services on a twenty-four hour basis, including holidays and weekends, in accordance with the Public Health Service, U.S. Department of Agriculture and American Association for Accreditation of Laboratory Animal care requirements. Computer: Excellent computer facilities in our laboratories, including Pentium III and IV PCs and Apple computers, access by hard line to the campus computer center, and access to the San Diego Supercomputer which is located on campus are available if necessary. Office: Dr. Michael Hogan has exclusive use of an office in the Medical Teaching Facility, across the hall from the laboratories. Other: Electron Microscopes are available on campus at the Veterans Administration Hospital and UCSD Scripps Institute of Oceanography. They are used on a recharge basis. We have used these microscopes over the past 20 years and they are satisfactory for our purposes. MAJOR EQUIPMENT: List the most important equipment items already available for this project, noting the location and pertinent capabilitiesof each. Permanent laboratory equipment relevant to this Core in the Division of Pulmonary Physiology includes three LKB Ultrotome III (thin sectioning for EM), LKB and RMC glass knife breakers, Jung Biocut 2035 microtome, Reichert Jung Cryocut 1800, Meiji EMZ Series dissecting microscope for trimming plastic EM blocks, Leitz Ortholux and Laborlux light microscopes equipped with Leica cameras, Jenalumar fluorescence microscope equipped with SPOT RT 215-3 camera, American Innovision and Image Pro Plus 4.5.1. image analyzers, Sony DXC 960MD color video camera, Sony UP-5600 MD color video printer, Jena and Bell Howel microfilm readers, Osmometer (Model 3W, Advanced Instruments), fume hood, darkroom, and Revco freezer -80 ¿C. Equipment for vascular perfusion and pressure recording are also available. For cellular imaging, two PTI fluorescent systems, a McBain Confocal Microscope system, and a system for phosphorecent imaging of intracellular PO2 (Oxygen Systems) are available for all Units. Wet biochemistry using standard NADH fluorescent techniques along with a HPLC are also within these labs. PHS 398/2590 (Rev. 04/06) Page 270 Resources Format Page