Circadian timekeeping in hamster mutants

仓鼠突变体的昼夜节律

• 批准号: 10790160
• 负责人: ERIC L BITTMAN
• 金额: $ 42.47万
• 依托单位: MARQUETTE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-18 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10790160
• 关键词: Affect; American; Animal Model; Behavior; Behavioral; Biology; Brain; Cardiovascular Diseases; Cell physiology; Circadian Dysregulation; Circadian Rhythms; Crying; Cues; Darkness; Disease; Feedback; Foundations; Gene Expression; Genes; Genetic Polymorphism; Genetic Transcription; Hamsters; Health; Human; Immunologic Deficiency Syndromes; Impaired cognition; Jet Lag Syndrome; Light; Link; Mammals; Melatonin; Mesocricetus auratus; Modeling; Molecular; Mus; Mutation; Nerve Degeneration; Nervous System Physiology; Obesity; Optics; Pathologic; Pathology; Phenotype; Physiological; Physiology; Process; Public Health; Rattus; Reporter; Reporter Genes; Risk; Rodent; Sleep; System; Testing; Tissues; Translating; Work; cancer risk; circadian; circadian pacemaker; day length; human disease; insight; interest; molecular clock; mouse model; mutant; novel; photoperiodicity; reproductive; response; screening; suprachiasmatic nucleus; tau Proteins

PROJECT ABSTRACT Aberrant light exposure disrupts daily rhythms in humans and is linked to many diseases. This is a serious public health concern because light at night impacts up to 80% of Americans. Further, polymorphisms in circadian genes alter sleep and responses to light, which produces high personal and societal burdens. Despite many gains in the field, a major gap remains in understanding how changes in molecular clock function translate into phenotype and pathology. One obstacle is the common use of mouse models that lack systemic cues present in humans and other mammals (e.g., melatonin). The Syrian hamster is a melatonin- competent rodent with an impressive repertoire of circadian and photoperiodic responses to light. Having developed the first circadian reporter in a hamster model, here we will investigate circadian timekeeping at the central and systems-level in this informative species. Further, we will leverage well-characterized mutations present in the hamster that affect circadian clock function, photoentrainment, and physiology. This work will lay the essential foundations to use this animal model to gain a more holistic understanding circadian and photoperiodic processes that impact health.

项目摘要