Multiplexed proteomics-based kinase assay development

R01GM146386-暑期本科生研究人员补充资料-项目摘要 这项补充计划是为了支持本科生的研究训练和专业发展。 研究员。这名学生(来自明尼苏达大学)将在家长助学金首席调查员的 实验室和合作者的实验室，将他们的培训嵌入这两个环境中。这应该会增强 通过明确和直接地将候选人的培训经验和项目进展联系起来 两个实验室之间的实验规划和执行，一个是基础科学，另一个是翻译 连接在一起。在这个补充项目中，学生将在L.Parker的不同条件下准备样品 实验室，在两个细胞系中测试不同的多肽浓度和孵育时间。这些样本和其他 多肽(包括未标记多肽和稳定同位素标记标准)将被带到S.Parker实验室，地址为 西奈雪松。应聘者将学习蛋白质组学的样品处理和制备，有针对性的并行 从仪表端和数据分析端进行的反应监测(PRM)分析，还具有 通过向实验室小组展示他们的项目来促进专业发展的机会，以及 在Cedars Sinai的高度动态、尖端的翻译环境中与科学家和医生互动 医疗中心。

R01GM146386 – Supplement for Summer Undergraduate Researcher – Project Summary This supplement project is to support the research training and professional development for an undergraduate researcher. The student (from University of Minnesota) will work across the parent grant principal investigator's laboratory and the collaborator's laboratory, to embed their training in both environments. This should enhance both the candidate's training experience and the project's progress by explicitly and directly linking the experimental planning and execution between the two labs, one basic science and one more translationally connected. In this supplement project, the student will prepare samples under varying conditions in the L. Parker lab, testing different peptide concentrations and incubation times in two cell lines. These samples and additional peptides (both unlabeled peptides and stable isotope labeled standards) will be brought to the S. Parker lab at Cedars Sinai. The candidate will learn the sample processing and preparation for proteomics, targeted parallel reaction monitoring (PRM) analysis both from the instrumentation side and the data analysis side, and also have opportunities to enhance professional development through presenting their project to the lab groups, and interact with scientists and physicians in the highly dynamic, cutting-edge translational setting at Cedars Sinai Medical Center.