Multiplexed proteomics-based kinase assay development

R 01 GM 146386-夏季本科研究员补充-项目摘要 这个补充项目是为了支持本科生的研究培训和专业发展 研究员该学生（来自明尼苏达大学）将在父母补助金首席研究员的工作 实验室和合作者的实验室，以嵌入他们的培训在这两个环境。这将增强 候选人的培训经验和项目的进展，通过明确和直接的联系， 两个实验室之间的实验计划和执行，一个是基础科学实验室，一个是实验室 连接.在这个补充项目中，学生将在不同的条件下制备样品。帕克 实验室，在两个细胞系中测试不同的肽浓度和孵育时间。这些样品和其他 将肽（未标记的肽和稳定同位素标记的标准品）带到S.帕克实验室在 雪松西奈。候选人将学习蛋白质组学的样品处理和制备，有针对性的平行 反应监测（PRM）分析从仪器侧和数据分析侧，也有 有机会通过向实验室小组展示他们的项目来加强专业发展， 在Cedars Sinai的高度动态，尖端的翻译环境中与科学家和医生互动 医学中心

R01GM146386 – Supplement for Summer Undergraduate Researcher – Project Summary This supplement project is to support the research training and professional development for an undergraduate researcher. The student (from University of Minnesota) will work across the parent grant principal investigator's laboratory and the collaborator's laboratory, to embed their training in both environments. This should enhance both the candidate's training experience and the project's progress by explicitly and directly linking the experimental planning and execution between the two labs, one basic science and one more translationally connected. In this supplement project, the student will prepare samples under varying conditions in the L. Parker lab, testing different peptide concentrations and incubation times in two cell lines. These samples and additional peptides (both unlabeled peptides and stable isotope labeled standards) will be brought to the S. Parker lab at Cedars Sinai. The candidate will learn the sample processing and preparation for proteomics, targeted parallel reaction monitoring (PRM) analysis both from the instrumentation side and the data analysis side, and also have opportunities to enhance professional development through presenting their project to the lab groups, and interact with scientists and physicians in the highly dynamic, cutting-edge translational setting at Cedars Sinai Medical Center.