Core C: Cell and Gene Vector Core

核心C：细胞和基因载体核心

• 批准号: 10396997
• 负责人: Sudarsan Rajan
• 金额: $ 27.74万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10396997
• 关键词: Adenoviruses; Adult; Cardiac Myocytes; Cells; Cloning; Communication; Dependovirus; Educational process of instructing; Genes; Grant; Heart Injuries; Heart failure; In Vitro; Lentivirus; Mus; Muscle Cells; Neonatal; Organ; Pathway interactions; Preparation; Production; Protocols documentation; Rattus; Reproducibility; Research; Research Personnel; Resources; Services; Signal Pathway; Structure of thyroid parafollicular cell; Syndrome; Techniques; Therapeutic; Ventricular; Viral; Viral Vector; Virus; Work; experience; experimental study; in vivo; innovation; large scale production; novel; repair strategy; sex; tissue culture; vector

Core C SUMMARY The Cell and Gene Vector Core (Core C) will provide an essential centralized support service to the investigators on the grant by constructing, propagating and large-scale production of adenoviral, lentiviral and adeno-associated viral (AAV) stocks, which all Projects will need. Utilizing the vast experience and expertise of the Core Director's, and in-house established protocols for the viral vector productions, the Core will provide a consistent and reproducible service in support of the projects. This Core will also house expertise for cultured myocytes for in vitro mechanistic work including weekly neonatal rat ventricular myocyte preparations and isolation of adult murine cardiomyocytes when needed. An additional benefit of the Core is that it provides a resource for teaching others tissue culture and viral production techniques. These essential centralized support services, used by all Projects, will eliminate variability in virus and myocyte preparations and will be pivotal in identifying novel signaling pathways, intra-organ communication pathways and sex-dependent mechanisms that contribute to heart failure and cardiorenal syndrome after cardiac injury and identify potential intersecting mechanisms to develop innovative therapeutic strategies for repair.

核心C摘要 细胞和基因载体核心（核心C）将为细胞和基因载体提供必要的集中支持服务。 研究人员通过构建、繁殖和大规模生产腺病毒、慢病毒和 腺相关病毒（AAV）库存，所有项目都需要。利用丰富的经验和专业知识， 核心总监和内部建立的病毒载体生产方案，核心将提供 为项目提供一致和可复制的服务。这一核心还将容纳专门知识， 用于体外机械工作的肌细胞，包括每周一次的新生大鼠心室肌细胞制备， 需要时分离成年鼠心肌细胞。核心的另一个好处是它提供了一个 组织培养和病毒生产技术的教学资源。这些重要的集中支持 所有项目使用的服务将消除病毒和肌细胞制剂的变异性，并将在以下方面发挥关键作用： 识别新的信号传导途径、器官内通讯途径和性别依赖机制， 导致心脏损伤后心力衰竭和心肾综合征，并确定潜在的交叉 机制，以开发创新的修复治疗策略。