Development of Human-Selective Nerve Illumination Peptide for Surgery

摘要 外科手术的一个基本目标是保留功能并将患者的发病率降至最低。 目前手术中的神经识别使用不可量化的标准，如解剖学、质地、 颜色，与周围结构的关系，以区分神经和非神经组织。在……里面 使用上述标准的创伤、肿瘤侵袭或感染、神经识别的情况可能是 很有挑战性。使用白光反射率，这是操作中的标准照明模式 房间内，小神经之间的视觉差异，如海绵体神经在 根治性前列腺切除术或面神经远端分支在涎腺手术中的重要作用 肿瘤和邻近组织可能是不可察觉的。我们的实验室有丰富的经验 开发用于神经外科可视化的分子靶向智能探针。最近，我们有 通过噬菌体展示鉴定出一种与人类神经高度结合的新多肽(HNP401)。在……里面 这项建议，我们的目标是优化HNP401，使其能够临床翻译为人类手术。

Abstract A fundamental goal of surgery is function preservation and minimizing patient morbidity. Current nerve identification during surgery utilizes non-quantifiable criteria such as anatomy, texture, color, relationship to surrounding structures to distinguish nerves from non-nerve tissues. In instances of trauma, tumor invasion or infection, nerve identification using the above criteria may be challenging. Using white light reflectance, which is the standard mode of illumination in operating rooms, the visual difference between small nerves, such as cavernosal nerves important during radical prostatectomy or distal branches of the facial nerve important during surgery for salivary gland neoplasms, and adjacent tissue can be imperceptible. Our laboratory has extensive experience in developing molecularly targeted smart probes for surgical visualization of nerves. Recently, we have identified a novel peptide (HNP401) via phage display to have high level of human nerve binding. In this proposal, we aim to optimize HNP401 to enable its clinical translation for human surgery.