Stanford Tissue Mapping Center

斯坦福大学组织绘图中心

• 批准号: 9788507
• 负责人: William James Greenleaf
• 金额: $ 57.76万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9788507
• 关键词: ATAC-seq; Antibodies; Architecture; Attention; Behavior; Biological Assay; Brain; Brain Death; Catalogs; Cells; Cellular Assay; Cellular Structures; Chromatin; Colon; Complex; Consent; Coupled; Data; Development; Dietary Fats; Dimensions; Disease; Epithelial; Epithelial Cells; Family; Gastrointestinal tract structure; Gene Expression; Geography; Human; Immune; Immune system; Individual; Inflammatory Bowel Diseases; Informed Consent; Intestines; Investigation; Large Intestine; Lead; Length; Lymphatic; Maps; Metabolism; Methods; Molecular; Morphology; Mucous Membrane; Muscle; Neurophysiology - biologic function; Organ; Organ Donor; Organ Preservation; Pacemakers; Phenotype; Physiology; Population; Protocols documentation; Regulator Genes; Research; Resolution; Resources; Role; Signaling Molecule; Small Intestines; Structure; Surface; Tissue Preservation; Tissues; Transplantation; Volatile Fatty Acids; base; body system; brain tissue; cell type; gastrointestinal; genome-wide; gut microbiota; human tissue; ileum; imaging approach; immunoregulation; lymph nodes; microbiome; microbiota; molecular dynamics; multiplexed imaging; nutrient absorption; programs; relating to nervous system; single-cell RNA sequencing; spatial relationship; transcriptome

ABSTRACT: Organ Specific Project Beyond nutrient absorption, the gastrointestinal tract has wide-ranging effects on the normal and diseased physiologies of other organ systems, including metabolism, neural function, and the immune system. A high-resolution map of the bowel would be an invaluable resource to understand normal bowel function and the perturbations that lead to disease. We propose to create this map along the length of the small bowel and colon, both of which have nuanced geographic specializations of function. Using single cell RNA-seq and single cell ATAC-seq, we will profile the gene expression and regulatory programs that define the complex cell populations that drive bowel function. We will use CODEX, a highly-multiplexed, antibody-based mapping method, to define the spatial relationships of these cell populations. These investigations will be performed on tissues that are preserved and procured in a manner suitable for human bowel transplantation. Are target milestone is to characterize the small bowel and colon tissues from a total of 22 individuals over the course of this four-year effort. Our protocol to obtain tissues from human organ donors whose families have provided broad, open access consent is in place, and we are actively collecting tissues for other studies.

摘要：器官特异性项目 除了营养吸收，胃肠道对正常人有广泛的影响。 和其他器官系统的疾病生理学，包括新陈代谢，神经功能， 免疫系统.一张高分辨率的肠道地图将是一个宝贵的资源， 了解正常的肠道功能和导致疾病的扰动。我们建议 沿着小肠和结肠的长度沿着创建这张地图， 功能的地理专业化。使用单细胞RNA-seq和单细胞ATAC-seq，我们 将描绘基因表达和定义复杂细胞的调控程序， 推动肠道功能的人群。我们将使用CODEX，一种高度多路复用的，基于抗体的 映射方法，以定义这些细胞群的空间关系。这些 研究将对以某种方式保存和获取的组织进行 适用于人体肠道移植。目标里程碑是表征小肠 和结肠组织，在这四年的努力中，总共有22个人。我们 从其家属提供了广泛、公开、 知情同意已经到位，我们正在积极收集组织用于其他研究。