Morphology and Imaging Module

摘要/摘要--形态和成像模块 形态和成像模块的主要功能是提供与专家人员和 用于固定和活体眼睛组织的显微研究的设备。拥有一套总共921间的房间 平方。英国《金融时报》在专用空间中，该模块通过提供最先进的技术来增强研究环境 为那些使用解剖学和成像技术的研究人员提供设备，并且由于技术上的 它提供了专业知识，使其他人能够将解剖学技术添加到他们的研究工具库中。 为用于1)眼组织的光和光处理的仪器提供通道和培训 共聚焦显微镜、免疫组织化学和原位杂交，以及2)捕获和处理 用光和共焦(常规和多光子)显微镜对活的和固定的组织成像。 该模块提供的设备包括两个低温恒温器、一个石蜡处理和包埋站以及 石蜡切割机，徕卡激光显微解剖显微镜，两个广场荧光显微镜(和 奥林巴斯EX-51显微镜和徕卡DM6000显微镜)，传统的徕卡TCS SP5共焦 和一个多光子徕卡TCS SP5共焦显微镜。此外，还有一台电脑 专用于图像分析的工作站，配备Leica AF、Metamorph、Imaris等独立版本 成像软件。 该模块配备了一名全职技术人员，他们提供加工和切片方面的帮助 组织、实验设计、使用光显微镜和荧光显微镜的培训以及 相关的成像软件以及仪器的维护。还为模块配备人员(20%的工作量，费用由 眼科)是密歇根大学形态和图像分析核心中心的经理 糖尿病和研究培训中心，提供徕卡SP5共焦使用方面的培训 显微镜和徕卡自动对焦、变形器和Imaris成像软件。形态与成像模块 在过去5年和16年中，17名参与调查人员适度或广泛地使用了 预计在下一个资助期内适度或广泛地使用该模块。

SUMMARY/ABSTRACT - MORPHOLOGY AND IMAGING MODULE The primary function of the Morphology and Imaging Module is to provide access to expert personnel and equipment for microscopic studies of both fixed and live ocular tissues. Housed in a suite of rooms totaling 921 sq. ft. of dedicated space, this Module enhances the research environment by making available state-of-the-art equipment for those investigators who use anatomical and imaging techniques, and, because of the technical expertise provided, it enables others to add anatomical techniques into their repertoire of research tools. Access and training are provided for instrumentation used in 1) the processing of ocular tissue for light and confocal microscopy, immunohistochemistry, and in situ hybridization, and 2) the capturing and processing of images of living and fixed tissue by light and confocal (conventional as well as multiphoton) microscopy. Equipment available in this module includes two cryostats, a paraffin processing and embedding station and paraffin microtome, a Leica laser microdissection microscope, two wide-field fluorescence microscopes (an Olympus EX-51 microscope and a Leica DM6000 microscope), a conventional Leica TCS SP5 confocal microscope, and a multiphoton Leica TCS SP5 confocal microscope. In addition, there is a computer workstation dedicated to image analysis with a stand-alone version of Leica AF, MetaMorph, Imaris and other imaging software. The Module is staffed by a full-time technician who provides assistance with the processing and sectioning of tissue, the design of experiments, training in the use of light and epifluorescence microscopes and associated imaging software, and maintenance of instruments. Also staffing the module (20% effort, paid by the Department of Ophthalmology) is the manager of the Morphology and Image Analysis Core of the Michigan Diabetes and Research Training Center, who provides training in the use of the Leica SP5 confocal microscopes and Leica AF, MetaMorph, and Imaris imaging software. The Morphology and Imaging Module was moderately or extensively utilized by 17 participating investigators during the past 5 years and 16 anticipate using the Module to a moderate or extensive degree in the next funding period.