A novel approach to detect exosome-localized proteins and its application in breast cancer detection

检测外泌体定位蛋白的新方法及其在乳腺癌检测中的应用

• 批准号: 10005258
• 负责人: Kenneth Kwon
• 金额: $ 20.1万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10005258
• 关键词: 3-Dimensional; 4T1; Adherent Culture; Biochemical; Biological; Biological Markers; Biology; Biotin; Breast; Breast Cancer Cell; Breast Cancer Detection; Breast Cancer cell line; Breast Carcinoma; Cardiovascular system; Cell Line; Cell membrane; Cells; Cellular biology; Cues; Data; Data Analyses; Data Collection; Disease; Early Diagnosis; Encapsulated; Environment; Extracellular Matrix; Future; Growth; Health; Human; Immune system; Immunologic Surveillance; In Vitro; Label; Ligase; Liquid substance; Malignant Neoplasms; Membrane; Messenger RNA; Methods; Molecular Profiling; Multivesicular Body; Mus; Natural Immunity; Neoplasm Metastasis; Nervous system structure; Organoids; Patients; Peptides; Pharmaceutical Preparations; Phenotype; Proteins; Proteome; Reporting; Research; Serum; Signaling Molecule; Staging; Techniques; Technology; Testing; Therapeutic; Untranslated RNA; Urinary system; Vesicle; Xenograft Model; Xenograft procedure; base; engineered exosomes; exosome; extracellular vesicles; genetic information; in vivo; innovation; malignant breast neoplasm; mouse model; new technology; novel; novel strategies; pathogen; public health relevance; response; tool; tumor microenvironment; vesicular release

Project Summary/Abstract Because exosomes mirror the origin and status of the cells, the analysis of the content encapsulated in exosomes from biological fluids can reveal information relevant to human health and disease. However, due to the paucity of highly effective and rigorous exosome isolation methods, identifying exosome content carrying biological information remains challenging. To advance our understanding of exosomes from breast cancer cells, we developed an innovative exosome isolation technology. With this new tool, we will investigate the function of exosomes in early detection and staging of breast cancer. In Aim 1 we will expand our new isolation technology that reports exosome proteome and validate the functionality extensively. In Aim 2, we will investigate how the release of exosomes is modulated in breast cancer cells in in vitro cultures and cell-derived xenografts. Successful data collection and analysis from this proposed study will not only determine the applicability of this novel technique but will also uncover the association between exosomes and breast cancer cell states, thus providing molecular signatures that can potentially serve as biomarkers and therapeutic choices for breast cancer.

项目总结/摘要 因为外泌体反映了细胞的起源和状态，所以对外泌体中包封的内容物的分析是非常重要的。 来自生物流体的外来体可以揭示与人类健康和疾病相关的信息。但由于 缺乏高效和严格的外泌体分离方法，鉴定携带外泌体的内容物， 生物信息仍然具有挑战性。为了促进我们对乳腺癌外泌体的理解， 细胞，我们开发了一种创新的外泌体分离技术。有了这个新工具，我们将调查 外泌体在乳腺癌早期检测和分期中的作用。在目标1中，我们将扩大新的隔离 报告外泌体蛋白质组并广泛验证功能的技术。在目标2中，我们将 研究如何在体外培养的乳腺癌细胞中调节外泌体的释放，以及细胞衍生的 异种移植这项拟议研究的成功数据收集和分析不仅将决定 这项新技术的适用性，但也将揭示外泌体与乳腺癌之间的关联 细胞状态，从而提供可以潜在地用作生物标志物和治疗剂的分子特征。 乳腺癌的治疗方法