Regulation of cGAS-Mediated Cytosolic DNA Sensing Pathway

cGAS 介导的胞质 DNA 传感途径的调节

• 批准号: 10054489
• 负责人: Shitao Li
• 金额: $ 10.47万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-03 至 2021-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10054489
• 关键词: Regulation; cGAS; Mediated; Cytosolic; DNA

Project summary Viral DNA and leaked mitochondrial DNA (mtDNA) can trigger the cytosolic DNA sensor, cyclic GMP-AMP synthase (cGAS) to initiate the interferon (IFN) production signal cascade. Aberrant activation of the DNA sensing pathway can result in inflammatory and autoimmune diseases, such as systemic lupus erythematosus. However, how host limits excessive or detrimental immune responses to viral DNA and mtDNA is not well elucidated. Thus, it is pressing to define the protective mechanisms that prevent aberrant activation of cGAS- mediated innate immunity. Our overall objective in this application is to elucidate a host regulatory feedback mechanism that prevents cGAS from over-activating. Pilot experiments identified the complement C1q binding protein (C1QBP), a protein predominantly expressed in mitochondria as being associated with cGAS. Our pilot studies found that knockout of C1QBP augmented DNA-induced IFN response, suggesting C1QBP negatively regulates cGAS signaling. Our preliminary studies further discovered that viral infection-induced mitochondrial stress resulted in the release of C1QBP from mitochondria to the cytoplasm. Based on the existing literature and our preliminary data, we propose the following central hypothesis: Mitochondrial stress causes the release of C1QBP from mitochondria into cytosol. Direct interaction between cytosolic C1QBP and cGAS inhibits type I IFN expression, thereby providing a mechanism that prevents aberrant or sustained activation of innate immune responses. Aim 1 will define the role of C1QBP in cytosolic DNA-mediated innate immunity in vitro and in vivo. Aim 2 will determine the protective mechanisms by which C1QBP limits cytosolic DNA-induced cGAS activation. cGAS activity must be tightly regulated because sustained IFN production can lead to autoimmune diseases. This exploratory R21 application proposes C1QBP as a hidden host protector in mitochondria matrix. When mitochondria are damaged by infection and other cellular stresses, C1QBP is released from mitochondria to inhibit cGAS activity, thereby preventing excessive IFN response triggered by the leaked mtDNA. This study will elucidate an elegant host self-regulation mechanism, providing foundations for developing novel therapeutic strategies for infectious and autoimmune diseases.

项目总结