Genotyping Core

基因分型核心

• 批准号: 10011812
• 负责人: DANIEL J CARR
• 金额: $ 11.49万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10011812
• 关键词: Awareness; Breeding; Catalogs; Center Core Grants; Complex; Consumption; Core Facility; DNA; Data; Data Analyses; Exercise; Future; Gene Deletion; Generations; Genes; Genetic Polymorphism; Genetic Screening; Genotype; Housing; Human Resources; Inbred Mouse; Mus; Mutant Strains Mice; Mutation; Pattern; Process; Protocols documentation; Research; Research Personnel; Scientist; Services; Standardization; Statistical Data Interpretation; Time; Transgenic Organisms; Vision; Vision research; Work; cost; ranpirnase; vision science

ABSTRACT Genotyping Core The Genotyping Core provides investigators comprehensive services in genotyping a broad range of transgenic and mutant mouse strains in a fast and accurate manner that is critical to the analysis and interpretation of the data. The generation of conditional deletion of genes involves a complex breeding pattern and creates multiple lines. Accurate genotyping is vital to validate the successful targeted gene or genes to identify the final genotypes. Efficient and timely analysis of mouse genotypes greatly facilitates the management of PI mouse colonies and allows for the culling of unwanted genotypes in a timely fashion. This process expedites the PIs to manage their mouse lines and reduce the costs of housing. The Genotyping Core allows us to take much of the burden of genotyping multiple lines from lab personnel and to streamline and better maintain our mouse colonies. A second major advantage is the expertise of screening for genetic polymorphisms expressed in several inbred mouse lines that can impact the results of our work, e.g., rd1 and rd8 mutations and rpe65 polymorphisms. The Genotyping Core is especially helpful to the junior investigators who may not have this expertise in their lab and cannot afford to hire someone for this service. Furthermore, multiple genotyping requires different protocols to analyze the genotypes: different protocols are a time- consuming exercise. The core will standardize these protocols for the PI and provide support to scientists in their genotyping projects in every aspect of research, including planning, extracting DNA, genotyping, interpreting data, and doing statistical analysis.

摘要 基因分型核心 基因分型核心为研究者提供广泛的基因分型综合服务， 转基因和突变小鼠品系，这对分析至关重要， 数据的解释。基因条件性缺失的产生涉及复杂的育种模式 并创建多条线。准确的基因分型对于验证成功的靶基因或基因以 确定最终的基因型。对小鼠基因型的有效和及时的分析极大地促进了 本发明的方法可以用于PI小鼠集落的管理，并允许及时剔除不需要的基因型。这 这一过程加快了PI管理其鼠标线并降低了住房成本。基因分型核心 使我们能够从实验室人员那里承担多条基因分型线的大部分负担， 更好地维护我们的老鼠群体。第二个主要优势是筛选遗传病的专业知识， 在几种近交系小鼠中表达的多态性可以影响我们的工作结果，例如，rd 1和 RD 8突变和RPE 65多态性。基因分型核心对初级研究人员特别有帮助 他们的实验室可能没有这种专业知识，也负担不起为这项服务雇人的费用。此外，委员会认为， 多重基因分型需要不同的方案来分析基因型：不同的方案是一次- 消耗运动。核心将为PI标准化这些协议，并为科学家提供支持， 他们的基因分型项目在研究的各个方面，包括规划，提取DNA，基因分型， 解读数据，做统计分析。