Dynamic interactions within alpha-herpesvirus virions and their impact on infection

项目总结 神经侵袭性α疱疹病毒，如单纯疱疹病毒(HSV)和伪狂犬病病毒 (PRV)在人类和其他动物中引起广泛的疾病，是重要的原因 发病率和死亡率。干扰病毒粒子结构重排的新策略 感染性所需的可能被证明对治疗感染很有价值，但病毒粒子的关键方面 架构及其亚稳性仍然没有明确的定义。在此应用程序中，我们概述了一种策略 以确定细胞外病毒颗粒内的动态蛋白质相互作用。拟议的研究 将检验基本病毒蛋白pUL36之间条件性相互作用的假设 衣壳在感染过程中起关键作用，包括病毒的分解和调节 PUL36脱泛素酶活性：阿尔法疱疹病毒建立终生所需的过程 神经系统的感染。

PROJECT SUMMARY Neuroinvasive alpha-herpesviruses such as herpes simplex virus (HSV) and pseudorabies virus (PRV) cause a broad range of diseases in humans and other animals and are significant causes of morbidity and mortality. Novel strategies to interfere with the virion structural rearrangements required for infectivity could prove valuable to treat infections, yet critical aspects of the virion architecture and its metastability remain poorly defined. In this application, we outline a strategy to identify dynamic protein interactions within extracellular viral particles. The proposed research will test the hypothesis that conditional interactions between the essential viral protein pUL36 and capsids contribute to critical steps in infection including viral disassembly and regulation of pUL36 deubiquitinase activity: processes required for alpha-herpesviruses to establish life-long infection of the nervous system.