Pathology Core

病理学核心(库克博士)将对所有研究的患者样本提供全面的中央病理学审查 项目1-4，利用基于网络的电子病理资源，并提供额外的辅助测试 在需要时进行分析，以得出一致的诊断。组织微阵列也将由 项目2和3的石蜡块，便于进行具有成本效益的下游分析。目标1)提供一种系统 使用数字幻灯片成像进行中央病理检查。由国际专家小组进行的中央审查 公认的淋巴瘤专家一直是LLMPP先前研究的基石之一。每一箱都是 由四名淋巴瘤病理学专家审查，并经至少三名病理学家同意定义为 共识诊断。对于未能达成初步共识的案件，由 整个病理委员会联合起来，并由多数人的意见建立了共识诊断。基于Web的 病理核心将利用位于克利夫兰临床电子病理中心的电子病理资源 以符合成本效益的方式促进中央审查。常规切片和免疫组织化学染色 使用Aperio整个幻灯片成像系统进行扫描，审查病理学家将可以访问 通过基于Web的界面查看分配给他们的案例。目标2)按要求完成辅助研究 以得出最终的病理诊断。附加的辅助测试可以包括免疫组织化学染色， 在某些情况下，可能需要进行荧光原位杂交(FISH)研究和/或淋巴克隆检测 得出明确的共识诊断的病例。当病理学家提出要求时，进行这种检测 进行中央审查，将在克利夫兰临床研究核心实验室进行，该实验室包括1 Ventana Discovery、3个Ventana Benchmark XT、2个Ventana Benchmark Ultra和1个DAKO 免疫组织化学平台。丰富的鱼类菜单和采用金标准的PCR克隆分析 欧洲克隆性入门套装也可供选择。目标3)通过以下方式促进个人研究项目 组织芯片的构建。组织微阵列将从提交给项目的样本中构建 2和3.詹姆斯·库克博士将担任病理核心的私人助理。库克博士在以下领域拥有丰富的经验 多机构研究的中心病理学回顾，曾是LLMPP淋巴瘤的成员 自2005年起担任病理委员会和SWOG淋巴瘤病理委员会委员。库克博士得到了一位 克利夫兰临床病理和检验医学研究所内的强大研究核心小组，包括 研究技术员、项目经理、会计专家和其他行政支持。这一核心将 通过确保研究的每个案例都经过彻底审查和 提供金标准、一致的病理诊断

The Pathology Core (Dr. Cook) will provide thorough central pathology review of all patient samples studied in Projects 1-4 of this grant, utilizing web-based e-pathology resources and providing additional ancillary testing analyses when required to arrive at a consensus diagnosis. Tissue microarrays will also be constructed from paraffin blocks for Projects 2 and 3, facilitating cost-efficient downstream analysis. Aim 1) To provide a system for central pathology review utilizing digital slide imaging. Central review by a panel of internationally recognized lymphoma experts has been one of the cornerstones of prior LLMPP studies. Each case is reviewed by four lymphoma pathology experts, and agreement by at least three pathologists defines a consensus diagnosis. For cases where an initial consensus is not reached, the cases are reviewed by the entire pathology committee together and a consensus diagnosis established by majority opinion. Web based e-pathology resources based in the Cleveland Clinic e-pathology center will be utilized by the Pathology Core to facilitate central review in a cost efficient manner. Routine sections and immunohistochemical stains will be scanned using an Aperio whole slide imaging system, and reviewing pathologists will be provided access to review their assigned cases through a web based interface. Aim 2) To complete ancillary studies as required to reach a final pathologic diagnosis. Additional ancillary testing may include immunohistochemical stains, fluorescence in situ hybridization (FISH) studies and/or lymphoid clonality testing, may be necessary in some cases to arrive at a definitive consensus diagnosis. Such testing, when requested by the pathologists performing central review, will be performed in the Cleveland Clinic research core laboratories which include 1 Ventana Discovery, 3 Ventana Benchmark XT, 2 Ventana Benchmark Ultra, and 1 Dako immunohistochemistry platforms. An extensive FISH menu and PCR clonality assays employing gold standard Euroclonality primer sets are also available. Aim 3) To facilitate individual research projects through construction of tissue microarrays. Tissue microarrays will be constructed from samples submitted for Projects 2 and 3. Dr. James Cook will serve as PI for the Pathology Core. Dr. Cook has extensive experience in central pathology review for multi-institutional studies, having been a member of the LLMPP lymphoma pathology committee and the SWOG lymphoma pathology committee since 2005. Dr. Cook is supported by a strong Research core group within the Cleveland Clinic Pathology and Laboratory Medicine Institute, including research technologists, project managers, accounting experts, and other administrative support. This Core will impact each of the Projects 1-4 in this grant by ensuring each case studied has been thoroughly vetted and providing a gold standard, consensus pathologic diagnosis