Penn integrated Human Pancreas procurement and Analysis Program
宾夕法尼亚大学综合人类胰腺采购和分析计划
基本信息
- 批准号:10063635
- 负责人:
- 金额:$ 556.93万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2016
- 资助国家:美国
- 起止时间:2016-09-20 至 2021-05-31
- 项目状态:已结题
- 来源:
- 关键词:Acinar CellAdolescentAgeArchivesAutoantibodiesB cell repertoireB-LymphocytesBar CodesBeta CellBioenergeticsBiologyBloodCD8-Positive T-LymphocytesCalciumCell CompartmentationCell LineCell surfaceCellsChromatinCollaborationsCommunitiesCyclic GMPCytometryDNADataData AnalysesData SetDatabasesDerivation procedureDiabetes MellitusDiagnosisEffector CellEndocrine systemEnhancersEpigenetic ProcessEtiologyFibroblastsFlow CytometryFrequenciesFunctional disorderFutureGenetic TranscriptionGenomicsGenotypeGlucagonHumanHyperinsulinismImaging technologyImmune systemImmunobiologyImmunologicsInfrastructureInsulinInsulin-Dependent Diabetes MellitusIslet CellMapsMeasurementMedical HistoryMedical RecordsMembrane PotentialsMemoryMetadataModalityMolecular ProfilingMultiplexed Ion Beam ImagingNatural HistoryNon-Insulin-Dependent Diabetes MellitusOrgan DonorOxygen ConsumptionPancreasPathogenicityPatientsPennsylvaniaPhenotypePhysiologicalPopulationProcessProtein AnalysisProteinsRecording of previous eventsRegulatory T-LymphocyteResearch PersonnelResolutionReview CommitteeRiskSamplingSourceSpleenStainsT memory cellT-LymphocyteT-Lymphocyte SubsetsTechnologyTissuesTumor-infiltrating immune cellsUniversitiesWorkbasebiobankcell typecombinatorialcostdata accessdata integrationdata resourcedata sharingdata submissiondata visualizationdraining lymph nodeepigenomicsexperiencehuman leukocyte antigen testinghuman tissueinduced pluripotent stem cellisletlymph nodesmembermitochondrial membranemultidimensional datanext generation sequencingnovelprogramsprotein biomarkersquantitative imagingsingle-cell RNA sequencingtissue processingtranscriptome sequencingtwo-dimensionalwhole slide imaging
项目摘要
Penn Human Pancreas Procurement and Analysis Program
Abstract
Utilizing our existing infrastructure and scientific collaborations, we have assembled 6 cores with
expertise ranging from pancreas procurement and islet isolation to data integration for a
comprehensive and integrated Human Pancreas Procurement and Analysis Program based at
the University of Pennsylvania. Core A will procure a spectrum of human pancreata and
detailed donor medical history; perform high resolution HLA typing by next generation
sequencing; isolate islets; and distribute islets and tissues to the other Cores for further analysis
or processing. Core B will perform physiological phenotyping on the isolated islets. Core C will
quantify and characterize memory T cell subsets by flow cytometry and single cell qPCR
analysis; characterize suppressive activity of Tregs and the ability of related effector cells to be
suppressed; B cell phenotyping; and generate chromatin accessibility maps of enhancers in
pathogenic cell types. Core D will perform multiple advanced modalities for the molecular
profiling of isolated islets including RNAseq and microRNAseq of sorted islet cell populations;
mass cytometry for single cell quantification of more than 20 cell surface and intracellular
markers; and single cell RNAseq. Core E will process tissues using multiple modalities that will
allow for analysis using advanced technologies such as multiplexed immunoflourescent
staining, combinatorial barcoded FISH (combFISH), whole slide imaging, and quantitative image
analysis of protein markers and immune cell infiltrates. This Core will adapt 2-dimensional
mass cytometry to pancreatic sections utilizing multiplexed ion beam imaging (MIBI) technology.
This Core will also archive tissues as well as DNA and blood, and facilitate sample distribution
to HPPAP approved researchers. Finally, Core F will assemble, annotate and maintain an open
access database for the Program and its member-researchers, and collaborate with the HIRN in
the sharing of data from both programs. The entire Program will be executed by an
Administrative Core consisting of the PIs, with assistance from an Executive Committee
consisting of the core leaders. The Administrative Core will interface with an external committee
to review applications for HPPAP biosample use, and will collaborate with the HIRN. The
Program will also interact with the HPPAP member/PANC DB user community to provide a
richly annotated source of physiologic, genomic and immunologic data on the tissue-based
landscape governing T1D.
Penn人类胰腺采购和分析计划
摘要
利用我们现有的基础设施和科学合作,我们已经组装了6个核心,
专业知识,从胰腺采购和胰岛分离到数据集成,
全面和综合的人类胰腺采购和分析计划,
宾夕法尼亚大学核心A将获得人类胰腺的光谱,
详细的供体病史;由下一代进行高分辨率HLA分型
测序;分离胰岛;并将胰岛和组织分配给其他核心进行进一步分析
或处理。核心B将对分离的胰岛进行生理表型分析。核心C将
通过流式细胞术和单细胞qPCR定量和表征记忆T细胞亚群
分析;表征THBE的抑制活性和相关效应细胞被抑制的能力。
抑制; B细胞表型;并生成增强子的染色质可及性图,
致病细胞类型。核心D将对分子进行多种高级模式
分析分离的胰岛,包括分选的胰岛细胞群的RNAseq和microRNAseq;
用于单细胞定量的质量细胞仪,可检测20多种细胞表面和细胞内
标记物;和单细胞RNAseq。核心E将使用多种方式处理组织,
允许使用先进的技术进行分析,例如多重免疫荧光
染色、组合条形码FISH(combFISH)、全载玻片成像和定量成像
蛋白质标记物和免疫细胞浸润的分析。这个核心将适应二维
利用多路离子束成像(MIBI)技术对胰腺切片进行质谱细胞计数。
该核心还将存档组织以及DNA和血液,并促进样本分配
HPPAP批准的研究人员。最后,核心F将组装,注释和维护一个开放的
访问该计划及其成员研究人员的数据库,并与HIRN合作,
共享两个项目的数据。整个程序将由一个
行政核心,由执行委员会协助,
由核心领导人组成。行政核心将与一个外部委员会联系
审查HPPAP生物样本使用申请,并将与HIRN合作。的
该计划还将与HPPAP成员/PANC DB用户社区互动,
基于组织的生理学、基因组学和免疫学数据的丰富注释来源
景观管理T1 D。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Michael R Betts其他文献
Dynamics of dendritic cells and T cells in HTLV-1-associated neuroinflammatory disease: implications in immunomodulatory therapies and diagnostic tools
- DOI:
10.1186/1742-4690-8-s1-a187 - 发表时间:
2011-06-06 - 期刊:
- 影响因子:3.900
- 作者:
Sharrón L Manuel;George Makedonas;Michael R Betts;Jay Gardner;James J Goedert;Zafar K Khan;Pooja Jain - 通讯作者:
Pooja Jain
Michael R Betts的其他文献
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{{ truncateString('Michael R Betts', 18)}}的其他基金
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评估 HIV 储存持久性的多组学策略
- 批准号:
10676525 - 财政年份:2023
- 资助金额:
$ 556.93万 - 项目类别:
Viral ASAPseq definition of CD4+ T cell viral reservoirs
CD4 T 细胞病毒库的 Viral ASAPseq 定义
- 批准号:
10634740 - 财政年份:2022
- 资助金额:
$ 556.93万 - 项目类别:
Viral ASAPseq definition of CD4+ T cell viral reservoirs
CD4 T 细胞病毒库的 Viral ASAPseq 定义
- 批准号:
10548385 - 财政年份:2022
- 资助金额:
$ 556.93万 - 项目类别:
Penn integrated Human Pancreas procurement and Analysis Program
宾夕法尼亚大学综合人类胰腺采购和分析计划
- 批准号:
9236460 - 财政年份:2016
- 资助金额:
$ 556.93万 - 项目类别:
Viral control mechanisms of HIV-specific T cells in HIV-infected lymph node
HIV感染淋巴结中HIV特异性T细胞的病毒控制机制
- 批准号:
9089892 - 财政年份:2015
- 资助金额:
$ 556.93万 - 项目类别:
Viral control mechanisms of HIV-specific T cells in HIV-infected lymph node
HIV感染淋巴结中HIV特异性T细胞的病毒控制机制
- 批准号:
9278105 - 财政年份:2015
- 资助金额:
$ 556.93万 - 项目类别:
CD4+ T and B cell mechanisms of influenza vaccine non-responsiveness in older adu
老年人流感疫苗无反应的 CD4 T 和 B 细胞机制
- 批准号:
8788501 - 财政年份:2014
- 资助金额:
$ 556.93万 - 项目类别:
CD4+ T and B cell mechanisms of influenza vaccine non-responsiveness in older adu
老年人流感疫苗无反应的 CD4 T 和 B 细胞机制
- 批准号:
8985652 - 财政年份:2014
- 资助金额:
$ 556.93万 - 项目类别:
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