System for Multiplex Protein:Protein Interaction Studies Modeled with Antibodies

多重蛋白质系统：用抗体建模的蛋白质相互作用研究

• 批准号: 8780923
• 负责人: MICHAEL P WEINER
• 金额: $ 22.25万
• 依托单位: AXIOMX, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-15 至 2015-12-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8780923
• 关键词: Affinity; Antibodies; Antigen Targeting; Antigens; Bioinformatics; Biological Markers; Biological Response Modifier Therapy; Cataloging; Catalogs; Cell surface; Cloning; Complementary DNA; Diagnostic; Emulsions; Epitopes; Future; Generations; Goals; Human; Integrase; Libraries; Methods; Modeling; Nucleic Acids; Organ; Organism; Peptides; Phase; Pilum; Proteins; Proteome; Protocols documentation; Reagent; Recombinant Antibody; Screening Result; Specific qualifier value; Speed; Study models; System; Technology; Testing; Therapeutic; Tissues; Transfer RNA; Viral; base; commercialization; design; instrument; interest; neoplastic cell; novel; protein protein interaction; public health relevance; reagent testing; research study; tumor; vector

DESCRIPTION (provided by applicant): The overarching goal of Phase I is to produce a method that will generate, identify and clone a catalog of protein-protein interactions in a single rapid experiment. In this proposal we will model the technology using antibody-antigen interactions. Recombinant antibody libraries against a proteome could be produced prior to any need, and then desired affinity reagents systematically cloned as needed. Libraries of recombinational biopanning integration product against a proteome could also be distributed along with primer sets against a set of barcodes for facile cloning. In future endeavors, the method will be used to clone tumor-cell or other diseased tissue cDNAs to use as an epitope library to quickly identify potential biomarkers. Successful completion of this phase will allow th commercialization of antibodies that can be used for diagnostic and therapeutic applications at a speed and comprehensiveness that is not easily achieved by current methods. Kits composed of vectors, reagents, strains and methods would be of commercial interest.

描述（由申请人提供）：第一阶段的总体目标是产生一种方法，该方法将在单个快速实验中生成，鉴定和克隆蛋白质-蛋白质相互作用目录。在本提案中，我们将使用抗体-抗原相互作用对该技术进行建模。针对蛋白质组的重组抗体文库可以在任何需要之前生成，然后根据需要系统地克隆所需的亲和试剂。针对蛋白质组的重组生物计划整合产物库也可以与针对一组条形码的引物集一起分发，以便于克隆。在未来的努力中，该方法将用于克隆肿瘤细胞或其他病变组织的cdna，作为快速识别潜在生物标志物的表位文库。这一阶段的成功完成将使抗体商业化，这些抗体可用于诊断和治疗应用，其速度和全面性是目前方法难以实现的。由载体、试剂、菌株和方法组成的试剂盒将具有商业价值。

项目成果

Platform for high-throughput antibody selection using synthetically-designed antibody libraries.

• DOI: 10.1016/j.nbt.2015.11.005
• 发表时间: 2016-09-25
• 期刊: New biotechnology
• 影响因子: 5.4
• 作者: Batonick M;Holland EG;Busygina V;Alderman D;Kay BK;Weiner MP;Kiss MM
• 通讯作者: Kiss MM