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Penn Center for Multi-scale Molecular Mapping of the Female Reproductive System

宾夕法尼亚大学女性生殖系统多尺度分子图谱中心

基本信息

批准号：
10117836
负责人：
JUNHYONG KIM
金额：
$ 75万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-24 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10117836
关键词：
3-Dimensional Anatomic Models Anatomy Atlases Basic Science Benign Biological Assay Cell Communication Cells Cellular biology Cervical Characteristics Chromatin Clinical Complex Data Deposition Discipline of obstetrics Endometrial Ensure Equilibrium Experimental Designs Fertility Generations Goals Gynecologic Gynecology Health Human BioMolecular Atlas Program Human Biology Image Individual Informatics Information Systems Location Malignant Neoplasms Mammalian Oviducts Maps Measurement Metadata Modeling Molecular Operative Surgical Procedures Organ Organoids Ovary Pennsylvania Physiological Procedures Process Prophylactic treatment Protocols documentation Quality Control RNA Radiology Specialty Reagent Records Reproductive Health Reproductive Medicine Reproductive system Research Research Personnel Research Subjects Resources Risk Sampling Site Sterilization Structure System Therapeutic Time Tissue Sample Tissues Transplantation United States Universities Uterus Women&apos s Health Work blastocyst cell community computational pipelines cost data sharing digital female reproductive system human female indexing individual variation member molecular scale multimodality programs single molecule single-cell RNA sequencing structured data transcriptomics

项目摘要

The female reproductive system, the uterus, fallopian tubes and the ovaries, is a complex interrelated set of organs that is physiologically dynamic and not only important for fertility but critically interrelated with general health. Single cell studies of the human female reproductive system and related tissues have been previously studied, but, as of yet, a comprehensive program, aligned with the goals of the HuBMAP, to define a molecular map of the entire system, integrating multi-modal assays, spatial diversity, and individual variations has not been established. The Penn Department of Obstetrics and Gynecology performs approximately 3,500 surgical procedures annually, of which many procedures allow sampling of multiple organs and locations from the same subject under normal conditions. Here, we propose to leverage the sampling opportunities afforded by the Penn ObGyn group and the single cell biology expertise of Penn investigators to establish a Penn Center for Multi-scale Molecular Map of the Female Reproductive System. We will obtain a comprehensive molecular characterization of the female reproductive system using six different molecular assays for at least ~700 tissue samples in anatomically indexed samples, creating a key resource for both basic science and women’s health. The molecular assays include single cell RNAseq, clampFISH spatial transcriptomics, simultaneous single cell open chromatin and RNA assays, and spatial open chromatin assay, among others. We will also generate a 3D anatomical model to provide spatial coordinate for our molecular characterization. All assay data will be registered to our 3D anatomical map that will be integrated with the HIVE Common Coordinate Framework. All metadata from subject records, clinical procedures, molecular procedures, and informatics pipelines will be collected, curated, and deposited as structured data. All data, including an extensive set of metadata, will be made available as a public resource. The completion of this resource will impact reproductive medicine for women’s health and also inform basic biology of human cell communities.

女性生殖系统，子宫，输卵管和卵巢，是一个复杂的相互关联的集合