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AAV-mediated delivery of eCD4-Ig for prevention and treatment of perinatal HIV infection

AAV 介导的 eCD4-Ig 递送用于预防和治疗围产期 HIV 感染

基本信息

批准号：
10082720
负责人：
Mauricio de Aguiar Martins
金额：
$ 83.55万
依托单位：
SCRIPPS FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-07 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10082720
关键词：
2 year old AIDS/HIV problem Address Adherence Adult Animals Antibodies Antiviral Agents Area Binding Birth Blood Chemical Analysis Blood Circulation Breast Breastfed infant CCR5 gene CXCR4 gene Caring Cell Count Cells Child Childhood Chronic Phase Data Dependovirus Early treatment Foundations Gene Transfer HIV HIV Entry Inhibitors HIV Infections HIV-1 HIV-2 Healthcare Human Immune Immunity Immunoglobulin G Immunoglobulin Genes Immunoglobulins Immunologic Deficiency Syndromes Infant Infant Health Infection Infrastructure Ingestion Interphase Cell Intervention Kinetics Macaca mulatta Mediating Modeling Monitor Monkeys Morbidity - disease rate Mother-to-child HIV transmission Mothers Neonatal Newborn Infant Oral Pathogenicity Pharmaceutical Preparations Placebos Postpartum Period Prevention Primate Lentiviruses Property Prophylactic treatment Reporting Reproducibility Research Resources SIV Safety Serum Skeletal Muscle Testing Time Transgenes Treatment-related toxicity Vaccines Vertical Disease Transmission Viremia Virus Virus Diseases Virus Replication Weight Gain adeno-associated viral vector antenatal antiretroviral therapy base combat env Gene Products experience experimental study gene therapy immunogenicity immunological intervention in vivo inhibitor/antagonist interest low income country mature animal mimetics mortality neonatal infection neonatal period neonate pediatric human immunodeficiency virus infection peptidomimetics perinatal HIV placebo group postnatal pre-clinical prenatal prevent programs prophylactic receptor simian human immunodeficiency virus therapy resistant transgene expression virtual

项目摘要

PROJECT SUMMARY Mother-to-child transmission of human immunodeficiency virus (HIV) still results in hundreds of thousands of new pediatric HIV infections every year, especially in resource-poor areas of the world where access to antenatal/postnatal antiretroviral therapy (ART) is limited. Sadly, in the absence of ART, >50% of HIV- infected infants die by 2 years of age. Since ART alone will not be sufficient to end the morbidity and mortality associated with HIV/AIDS in children, there is an urgent need for developing practical interventions to prevent and treat pediatric HIV infection. To that end, this project will evaluate the potential of gene therapy with the potent and extremely broad HIV inhibitor eCD4-Ig to prevent and treat perinatal HIV infection. eCD4-Ig is a chimeric molecule consisting of the outer domains of CD4, an IgG Fc portion, and a co-receptor mimetic peptide. eCD4-Ig has unmatched breadth and very potent effector activities against HIV-1, HIV-2, and SIV. The eCD4-Ig gene will be delivered to infant rhesus macaques (RMs) via adeno-associated virus (AAV)-mediated gene transfer. AAV vectors are safe and can transduce both dividing and non-dividing cells. Critically, AAV-driven transgene expression in long-lived cells, such as those of skeletal muscle, can last for years, possibly decades. While AAV-mediated delivery of eCD4-Ig has been shown to protect adult RMs against challenge with pathogenic immunodeficiency viruses bearing highly divergent Envelope proteins, this approach has never been tested in infants. We have recently partnered with the Farzan lab to characterize the kinetics of eCD4-Ig expression in infant RMs treated with AAV/eCD4-Ig at birth. Compared to adult animals, AAV/eCD4-Ig-treated newborn RMs experienced substantially higher levels of eCD4-Ig and lower levels of antibodies against the eCD4-Ig molecule. Note that these anti-drug antibodies (ADAs) are highly detrimental to AAV-mediated delivery of immunoglobulins because they can clear the molecules from circulation, thereby reducing the efficacy of this approach. The low levels of ADAs observed in the AAV/eCD4-Ig-treated infants is consistent with previous reports of neonates developing tolerance to AAV-delivered transgene products. Given the ability of AAV vectors to promote sustained transgene expression after a one-time administration, and the unique window of opportunity offered by the neonatal period to achieve robust AAV-driven expression of eCD4-Ig in vivo, we postulate that AAV-mediated delivery of eCD4-Ig to infants can prevent and treat postpartum HIV infection. In specific aim (SA) 1, we will characterize the safety profile of AAV/eCD4-Ig in infant RMs. In SA 2, we will determine if neonatal delivery of AAV/eCD4-Ig can prevent oral acquisition of SIVmac239 in RMs. In SA 3, we will assess if AAV-mediated delivery of eCD4-Ig to SIV-infected infant RMs can control viral replication without ART. If successful, the proposed experiments will build the pre-clinical foundation for testing AAV-mediated gene therapy with eCD4-Ig in infants as a means to prevent and treat perinatal HIV infection.

项目总结