Cell Engineering Shared Resource

细胞工程共享资源

• 批准号: 10092989
• 负责人: Ravi N Singh
• 金额: $ 4.51万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 2022-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10092989
• 关键词: Adenoviruses; Baptist Church; Cancer Center Support Grant; Cancerous; Cell Culture Techniques; Cell Line; Cells; Comprehensive Cancer Center; Cost Savings; Culture Media; Custom; Doctor of Philosophy; Equipment; Future; Gene Expression; Genes; Grant; Individual; Laboratories; Lentivirus; Mission; Molecular Biology; Mutation; Phenotype; Principal Investigator; Procedures; Production; Productivity; Quality Control; Reagent; Research; Research Personnel; Research Project Grants; Research Support; Resource Sharing; Retroviridae; Services; Site; System; Techniques; Viral; Viral Vector; Work; anticancer research; base; cell repository; cellular engineering; cellular transduction; cost effective; forest; member; repository

CELL AND VIRAL VECTOR LABORATORY SHARED RESOURCE Project Summary The mission of the Cell and Viral Vector Laboratory (CVVL) Shared Resource is to serve the scientific needs of Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) researchers by offering customized assistance for specialized cell culture and viral vector production; and by providing quality control for each research project. The use of primary and established cultures from cancerous and non-cancerous origins is essential for cancer research and requires careful quality control to maintain appropriate phenotype and functions. In addition, the use of various viral vectors (including lentivirus, retrovirus and adenovirus) has become a critical experimental technique for cancer research to effectively manipulate gene expression and to create desired mutations in particular genes. The CVVL also serves as a central site for purchasing and distributing specific culture media/reagents, molecular biology kits, and standard laboratory supplies in a cost- effective manner for WFBCCC members. The Specific Aims of CVVL are to 1) provide specialized cell culture services that support WFBCCC members' research needs, in an efficient and cost-effective manner; 2) maintain a cell repository of commonly used cell lines for WFBCCC member use; 3) provide WFBCCC members access to specialized equipment not readily available or not cost-effective for individual researchers; and 4) serve as a distribution center for critical cell culture and molecular biology reagents. The CVVL is extensively utilized by more than 139 Principal Investigators (both WFBCCC members and non-members) annually; 63% of services support the research of WFBCCC members. Services are provided to investigators on all institutional campuses at substantial cost savings with work conducted under stringent standard operating procedures. The expertise of the CVVL Director, Purnima Dubey, Ph.D., and the efforts of two technicians (1.5 FTE) provide high-quality cell culture and viral vector production services to researchers at WFBCCC. In the future, the CVVL plans to extend the viral production service by expanding its cutting-edge viral vector systems.

细胞和病毒载体实验室共享资源 项目摘要 细胞和病毒载体实验室（CVVL）共享资源的使命是为以下科学需求服务： 维克森林浸信会综合癌症中心（WFBCCC）的研究人员通过提供定制的 协助专门的细胞培养和病毒载体生产;并为每一项提供质量控制 研究项目。使用来自癌性和非癌性来源的原代培养物和已建立的培养物， 对于癌症研究至关重要，需要仔细的质量控制以保持适当的表型， 功能协调发展的此外，各种病毒载体（包括慢病毒、逆转录病毒和腺病毒）的使用已被广泛应用。 成为癌症研究的关键实验技术，以有效地操纵基因表达， 在特定基因中产生所需的突变。CVVL还作为采购和 分发特定的培养基/试剂、分子生物学试剂盒和标准实验室用品， WFBCCC成员的有效方式。CVVL的具体目的是：1）提供专门的细胞培养 以有效和具有成本效益的方式支持WFBCCC成员的研究需求的服务; 2） 维护常用细胞系的细胞库供WFBCCC成员使用; 3）提供WFBCCC 成员获得对个人研究人员来说不容易获得或不具成本效益的专门设备; 以及4）作为关键细胞培养和分子生物学试剂的配送中心。CVVL是 被超过139名主要研究者（WFBCCC成员和非成员）广泛使用 63%的服务支持WFBCCC成员的研究。向调查人员提供服务 在严格的标准下进行工作，节省大量成本 操作程序。CVVL总监Purnima Dubey博士的专业知识，两个人的努力 技术人员（1.5 FTE）为研究人员提供高质量的细胞培养和病毒载体生产服务， WFBCCC。未来，CVVL计划通过扩大其尖端技术， 病毒载体系统。