OLYMPUS FV1000 INVERTED SPECTRAL CONFOCAL SYSTEM

奥林巴斯 FV1000 倒光谱共焦系统

• 批准号: 7794254
• 负责人: JOHN A MERCER
• 金额: $ 37.48万
• 依托单位: MC LAUGHLIN RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-05 至 2011-08-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794254
• 关键词: Alzheimer' s Disease; Biomedical Research; Brain; Color; Conflict (Psychology); Confocal Microscopy; Demyelinating Diseases; Dyes; Embryo; Faculty; Funding; Hour; Image; Immunofluorescence Immunologic; In Situ Hybridization; Institution; Label; Manufacturer Name; Methods; Microscope; Natural regeneration; Nerve Fibers; Nervous System Trauma; Parkinson Disease; Peripheral Nerves; Peripheral Nervous System Diseases; Proteins; Request for Applications; Research; Research Institute; Schedule; System; programs

DESCRIPTION (provided by applicant): Confocal microscopy has become an important part of many biomedical research programs. The McLaughlin Research Institute last acquired a confocal unit (Bio-Rad MicroRadiance) twelve years ago. It has multiple limitations, including two colors, poor sensitivity, and lack of manufacturer support. Performing confocal microscopy at other research institutions requires more than a three-hour drive one way. This application requests funds for an Olympus FV1000 Spectral Confocal with IX81 inverted microscope and accessories. This microscope will allow imaging of peripheral nerve sections, teased nerve fibers, neurospheres, brain sections, and embryos labeled by in situ hybridization, immunofluorescence, vital dyes, and tagged proteins. These methods are critical to advancing NIH-funded projects studying mechanisms underlying nervous system injury and regeneration, Alzheimer's disease, Parkinson's disease, demyelinating diseases, and peripheral neuropathies. The addition of another microscope also will alleviate scheduling conflicts and allow for further expansion, with two new faculty planned in the next two years.

描述（由申请人提供）：共聚焦显微镜已成为许多生物医学研究计划的重要组成部分。麦克劳克林研究所上一次获得了十二年前的共聚焦单位（Bio-Rad微生物）。它具有多种限制，包括两种颜色，敏感性不佳以及缺乏制造商的支持。在其他研究机构进行共聚焦显微镜检查需要单程超过三个小时的驱动器。该申请要求与IX81倒置显微镜和配件的Olympus FV1000光谱共同体资金。该显微镜将允许对周围神经切片，嘲笑的神经纤维，神经球，脑切片和胚胎进行成像，并由原位杂交，免疫荧光，生命染料和标记的蛋白质进行标记。这些方法对于促进NIH资助的项目研究了神经系统损伤和再生，阿尔茨海默氏病，帕金森氏病，脱髓鞘疾病和周围神经病至关重要。增加另一个显微镜还将减轻日程安排冲突并允许进一步扩展，并计划在未来两年内进行两个新教师。