In Vivo MicroCT Scanner

体内 MicroCT 扫描仪

• 批准号: 7797486
• 负责人: ROBERTO PACIFICI
• 金额: $ 49.35万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-15 至 2011-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7797486
• 关键词: 1-Phosphatidylinositol 3-Kinase; Acquired Immunodeficiency Syndrome; Animals; Bone Diseases; Brain Neoplasms; Charge; Collaborations; Complement; Data; Estrogens; Funding; Grant; HIV; Hand; Image; In Vitro; Life; Maintenance; Malignant Neoplasms; Measurement; Metastatic Neoplasm to the Bone; Methods; Minor; Modeling; Mus; Organ; Osteogenesis; Osteoporosis; Pharmaceutical Preparations; Research; Research Personnel; Role; Running; Sample Size; Savings; Scanning; Structure; T-Lymphocyte; TNF gene; TNFRSF5 gene; Training; Universities; bone; heart function; improved; in vivo; indexing; instrument; interest; kinase inhibitor; nanoparticle; oncology; operation; programs; public health relevance; tumor growth

DESCRIPTION (provided by applicant): We request funds to purchase a Scanco viva MicroCT model 40, a devise which makes it possible to scan live small animals with the purpose of imaging organs, evaluate heart function, quantify trabecular and cortical bone and obtain information about bone structure. It also allows rapid and accurate measurements of histomorphometric indices of bone volume, connectivity and structure. This instrument will be utilized by 7 major users [Drs. R. Pacifici (P.I.), G. Beck, L.W.K. Chung, M.S. Nanes, Dr. H. Shim M.N. Weitzmann, W. Lewis, who are PI for 7 RO-1s and 1 P0-1, 1 P20, 1 P50 and 1 VA merit grant, and run research programs, on bone, HIV- AIDS, nanoparticles, and cancer at Emory University. In addition, there will be 2 minor users, Drs. D. Durden, and M. Goodman who are P1 of 1 ro1, 1 P50 and a R56 grants, and have interests in oncology. Dr. Robert Guldberg, a collaborator of the P.I. and an expert on MicroCT will be in charge of the maintenance and operations of the instrument. Dr. Pacifici will utilize microCT in studies on the role of T cells in the mechanism of action of PTH and estrogen. Dr. Chung will utilize the microCT to study bone metastasis, Dr. Nanes will use microCT to determine the mechanism by which TNF blocks bone formation. Dr. Shim will utilize the microCT to visualize tumor growth, Dr. Beck and Weitzmann will be aided in their studies on the effects of nanoparticles in bone. Dr. Lewis will use microCT to assess heart function in mice treated with HIV drugs. Dr. Durden will study the effects of PI-3 kinase inhibitors on brain tumors. Dr. Goodman will utilize the microCT to visualize brain tumors. In vivo MicroCT will facilitate each of these projects as it will complement and/or substitute in vitro microCT and bone histomorphometry, and 2D echo for heart function analysis. The advantages of having a microCT are several. 1) It would make possible to expand the planned studies with in vivo data about bone structure, a capability which is currently not on hand. 2) The greater sensitivity and precision of microCT over other methods would results in increased group separation and thus increased ability to interpret the data in a conclusive manner. 3) In some cases it may be possible to decrease the duration of the studies or the sample size with considerable financial savings. 4) We would be able to conduct our studies at a much faster pace. 5) The increased capability of in vivo microCT would provide additional opportunity for collaborations, training of new investigators and opening new fields of research. PUBLIC HEALTH RELEVANCE: Access to an in vivo microCT would allow researchers into causes and cures for osteoporosis and other bone disorders, to expand their studies with data about bone structure from live animals, gain access to greater sensitivity and precision which would results in increased group separation and conclusive interpretation, decrease the duration of studies or the sample size with considerable financial savings, improve the ability to conduct studies at a much faster pace, add additional opportunity for collaborations and training of new investigators which may open new fields of research.

描述（由申请人提供）：我们申请资金购买一台Sévio viva MicroCT型号40，这是一种能够扫描活体小动物的装置，目的是成像器官，评估心脏功能，量化骨小梁和皮质骨，并获得有关骨结构的信息。它还允许快速准确地测量骨体积、连接性和结构的组织形态计量学指数。该仪器将由7个主要用户使用[R. Pacifici（P.I.），G.贝克，L.W.K. Chung，M.S.纳内斯博士Shim M.N. Weitzmann，W.刘易斯，谁是PI的7个RO-1和1个P0-1，1个P20，1个P50和1个VA优秀奖助金，并运行研究计划，对骨，艾滋病毒-艾滋病，纳米粒子，和癌症在埃默里大学。此外，将有2个小用户，博士。Durden和M.古德曼谁是P1的1 ro 1，1 P50和R56赠款，并在肿瘤学的利益。罗伯特·古德伯格博士，私家侦探的合作者。一名MicroCT专家将负责仪器的维护和操作。Pacifici博士将利用microCT研究T细胞在PTH和雌激素作用机制中的作用。Chung博士将利用microCT研究骨转移，Nanes博士将利用microCT确定TNF阻断骨形成的机制。Shim博士将利用microCT可视化肿瘤生长，Beck博士和Weitzmann将协助他们研究纳米颗粒在骨中的作用。刘易斯博士将使用microCT来评估接受艾滋病毒药物治疗的小鼠的心脏功能。Durden博士将研究PI-3激酶抑制剂对脑肿瘤的影响。古德曼医生将利用微型CT来观察脑肿瘤。体内MicroCT将促进这些项目中的每一个，因为它将补充和/或替代体外microCT和骨组织形态测量，以及用于心脏功能分析的2D回波。使用microCT的优势有几个。1)这将有可能扩大计划的研究与体内数据有关的骨结构，这是目前没有的能力。2)microCT比其他方法更高的灵敏度和精确度将导致组分离增加，从而提高以决定性方式解释数据的能力。3)在某些情况下，可能会减少研究的持续时间或样本量，从而节省大量资金。4)我们将能够以更快的速度进行研究。5)体内microCT能力的提高将为合作、培训新的研究人员和开辟新的研究领域提供额外的机会。 公共卫生相关性：获得体内microCT将使研究人员能够研究骨质疏松症和其他骨骼疾病的原因和治疗方法，利用活体动物的骨骼结构数据扩展他们的研究，获得更高的灵敏度和精确度，这将导致增加组分离和结论性解释，减少研究持续时间或样本量，节省大量资金，提高以更快的速度进行研究的能力，增加合作和培训新研究人员的额外机会，这可能会开辟新的研究领域。