Qiagen 96-Sample Pyrosequencer

描述（由申请人提供）：所需设备：本提案寻求对收购Biotage/Qiagen生产的Pyromark Q96 MD自动焦磷酸测序系统的支持。该系统将允许大量NIH支持的研究人员在表观遗传学研究中对DNA甲基化进行高通量定量。原理：焦磷酸测序是一种实时“合成测序”技术，特别适用于DNA甲基化的定量分析。该用户组对环境因素及其对健康影响的机制进行研究，包括对与发育、肿瘤和功能变化有关的表观遗传修饰进行比较分析。焦磷酸测序将为研究人员提供高通量和高精度的全球和位点特异性甲基化定量分析的能力。实施情况：该仪器将位于一个完善的核心设施在目前的运作和支持的NIEHS中心赠款。仪表将由PI管理，PI负责NIEHS核心设施的管理，并在核心设施操作方面具有丰富的经验，以使用户受益。两名具有细胞和分子生物学经验的高级技术人员将接受系统培训，并将为用户组实验室成员提供培训、监督和支持。支持包括核心设施（机构）内的仪器空间，服务合同的覆盖范围和系统（机构）的未来组件更换，以及核心设施工作人员支持（NIEHS中心赠款）。一个内部咨询委员会将监督该系统的使用，并确保能够使用该系统。总结：该仪器将通过一个机构支持的、完善的核心设施，显着提高大量NIH支持的研究人员的研究能力和生产力。

DESCRIPTION (provided by applicant): Equipment Requested: This proposal seeks support for acquisition of a Pyromark Q96 MD automated pyrosequencing system made by Biotage/Qiagen. The system will allow high-throughput quantitation of DNA methylation in epigenetic studies by a large number of NIH-supported researchers. Rationale: Pyrosequencing is a real-time 'sequencing-by-synthesis' technology that is especially useful for quantitative analysis of DNA methylation. The user group conducts studies of environmental agents and the mechanisms of their health effects, including comparative analysis of epigenetic modifications linked to developmental, neoplastic and functional changes. Pyrosequencing will offer the investigators the ability to perform high-throughput and quantitative analyses of global and site-specific methylation with great precision. Implementation: The instrumentation will be situated within a well-established core facility in current operation and supported by a NIEHS Center grant. The instrumentation will be managed by the PI who directs the NIEHS core facilities, and has extensive experience in core facility operation for user benefit. Two senior technologists with experience in cell and molecular biology will be trained on the system and will provide training, supervision and support to the user group lab members. Support includes space for the instrumentation within the core facility (institutional), coverage of service contracts and future component replacements for the system (institutional), and core facility staff support (NIEHS Center grant). An internal advisory committee will supervise use and assure access to the system. Summary: This instrumentation will significantly improve the research capacity and productivity of a large number of NIH-supported investigators via an institutionally supported, well-established core facility.