Qiagen 96-Sample Pyrosequencer

Qiagen 96 样品焦磷酸测序仪

• 批准号: 7794497
• 负责人: LESTER KOBZIK
• 金额: $ 16.84万
• 依托单位: HARVARD SCHOOL OF PUBLIC HEALTH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-25 至 2011-03-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794497
• 关键词: Advisory Committees; Contract Services; Core Facility; DNA Methylation; Development; Epigenetic Process; Equipment; Future; Grant; Health; Link; Methylation; Modification; Molecular and Cellular Biology; National Institute of Environmental Health Sciences; Productivity; Request for Proposals; Research; Research Personnel; Sampling; Site; Supervision; System; Technology; Time; Training; comparative; environmental agent; experience; improved; instrumentation; member; neoplastic; operation

DESCRIPTION (provided by applicant): Equipment Requested: This proposal seeks support for acquisition of a Pyromark Q96 MD automated pyrosequencing system made by Biotage/Qiagen. The system will allow high-throughput quantitation of DNA methylation in epigenetic studies by a large number of NIH-supported researchers. Rationale: Pyrosequencing is a real-time 'sequencing-by-synthesis' technology that is especially useful for quantitative analysis of DNA methylation. The user group conducts studies of environmental agents and the mechanisms of their health effects, including comparative analysis of epigenetic modifications linked to developmental, neoplastic and functional changes. Pyrosequencing will offer the investigators the ability to perform high-throughput and quantitative analyses of global and site-specific methylation with great precision. Implementation: The instrumentation will be situated within a well-established core facility in current operation and supported by a NIEHS Center grant. The instrumentation will be managed by the PI who directs the NIEHS core facilities, and has extensive experience in core facility operation for user benefit. Two senior technologists with experience in cell and molecular biology will be trained on the system and will provide training, supervision and support to the user group lab members. Support includes space for the instrumentation within the core facility (institutional), coverage of service contracts and future component replacements for the system (institutional), and core facility staff support (NIEHS Center grant). An internal advisory committee will supervise use and assure access to the system. Summary: This instrumentation will significantly improve the research capacity and productivity of a large number of NIH-supported investigators via an institutionally supported, well-established core facility.

描述(由申请人提供)：所需设备：本提案寻求支持购买由Biotage/Qigen制造的PYROMARK Q96 MD自动焦解测序系统。该系统将允许由NIH支持的大量研究人员在表观遗传学研究中高通量量化DNA甲基化。原理：焦磷酸测序是一种实时的“合成测序”技术，对DNA甲基化的定量分析特别有用。用户群体对环境制剂及其对健康影响的机制进行研究，包括与发育、肿瘤和功能变化有关的表观遗传修饰的比较分析。焦磷酸测序将为研究人员提供高通量和高精度的全球和特定部位甲基化的定量分析能力。实施：该仪器将位于目前运作良好的核心设施内，并得到NIEHS中心赠款的支持。仪器将由指导NIEHS核心设施的PI管理，并在核心设施运营方面拥有丰富的经验，以造福用户。两名具有细胞和分子生物学经验的高级技术人员将接受关于该系统的培训，并将为用户组实验室成员提供培训、监督和支持。支助包括核心设施内仪器的空间(机构)、服务合同和系统未来部件更换的覆盖范围(机构)，以及核心设施工作人员支助(NIEHS中心赠款)。一个内部咨询委员会将监督系统的使用并确保访问该系统。摘要：这种仪器将通过一个制度上支持的、建立良好的核心设施，显著提高NIH支持的大量研究人员的研究能力和生产率。