Acquisition of a Pulse Electron Paramagnetic Resonance Spectrometer

脉冲电子顺磁共振波谱仪的采集

• 批准号: 7840304
• 负责人: Peter Z Qin
• 金额: $ 103.99万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-07 至 2011-12-06
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7840304
• 关键词: Alzheimer' s Disease; American; Area; Arts; Award; Cell Nucleus; Chromosomal translocation; Communities; Complex; DNA-Directed DNA Polymerase; Electron Nuclear Double Resonance; Electron Spin Resonance Spectroscopy; Electrons; Funding; Grant; Human; Infection; Institution; Ions; Ligands; Los Angeles; Maps; Measurement; Measures; Metals; Minority; Mission; Molecular; Molecular Conformation; Monitor; Nitrogenase; Occupations; Physiologic pulse; Public Health; Research; Spectrum Analysis; Structure; System; Training; Transcriptional Regulation; United States National Institutes of Health; Viral; biological systems; cofactor; instrument; protein transport; tool

DESCRIPTION (provided by applicant): The bio-molecular research community in the Los Angeles area is requesting NIH funding to purchase a state-of-art pulse electron paramagnetic resonance (EPR) spectrometer, the EleXsys FT-EPR system from Bruker Corporation. Pulse EPR spectroscopy is a powerful tool for analyzing conformations and metal ion cofactors of biological systems. The proposed pulse EPR spectrometer simultaneously will provide two capabilities that are central to the participating users. One is Double Electron Electron Resonance (DEER) spectroscopy, which selectively measures electron spin dipolar interactions to yield inter-spin distances up to 80 ¿ in bio-molecules. The measured distances provide direct structural constraints for mapping global structures and monitoring conformational changes of complex bio-molecular systems. User projects that require DEER span many important public health related areas, including viral replication and infection (X. Chen, Goodman, Qin), protein trafficking (Shan), transcription regulation (L. Chen), and human chromosomal translocation (Lieber). The second type of pulse EPR measurement is Electron Nuclear Double Resonance (ENDOR) and Electron Spin Echo Envelope Modulation (ESEEM), which measure interactions between the electron spin and the surrounding nuclei. This will be used to study ligand identity and coordination geometry of metal co-factors in nitrogenase (Rees, McKenna), DNA polymerase (McKenna, Crans, Goodman), and amoyloid formation in Alzheimer's disease (Zhou). Pulse EPR capabilities are grossly inadequate at the participating institutions. Funding of the proposed instrument will significantly benefit on-going research in the group of users, which are currently supported by 23 NIH grants. It will also support the broader regional research community, training of minorities, and educational missions. The award will immediately impact approximately 500 jobs in American, thus stimulating the economy.

描述（由申请人提供）：洛杉矶地区的生物分子研究团体正在请求NIH资助购买布鲁克公司最先进的脉冲电子顺磁共振（EPR）光谱仪，即EleXsys FT-EPR系统。脉冲电子顺磁共振波谱是分析生物体系构象和金属离子辅因子的有力工具。拟议中的脉冲EPR光谱仪同时将提供两种能力，这两种能力对参与用户至关重要。一种是双电子电子共振（DEER）光谱，它选择性地测量电子自旋偶极相互作用，以产生生物分子中高达80 º的自旋间距离。测量的距离提供了直接的结构约束映射全球结构和监测复杂的生物分子系统的构象变化。需要DEER的用户项目跨越许多重要的公共卫生相关领域，包括病毒复制和感染（X。Chen，Goodman，Qin）、蛋白质运输（Shan）、转录调控（L. Chen）和人染色体易位（Lieber）。第二种类型的脉冲EPR测量是电子核双共振（ENDOR）和电子自旋回波包络调制（ESEEM），它们测量电子自旋与周围原子核之间的相互作用。这将用于研究固氮酶（Rees，McKenna），DNA聚合酶（McKenna，Crans，Goodman）和阿尔茨海默病（Zhou）中淀粉样蛋白形成中金属辅因子的配体身份和配位几何结构。参与机构的脉冲EPR能力严重不足。拟议工具的资金将大大有利于用户组正在进行的研究，这些研究目前得到23个NIH赠款的支持。它还将支持更广泛的区域研究界、少数民族培训和教育特派团。该奖项将立即影响美国约500个工作岗位，从而刺激经济。

项目成果

CRISPR-Cas9 Mediated DNA Unwinding Detected Using Site-Directed Spin Labeling.

使用定点自旋标记检测 CRISPR-Cas9 介导的 DNA 解旋。

• DOI: 10.1021/acschembio.6b01137
• 发表时间: 2017
• 期刊: ACS chemical biology
• 影响因子: 4
• 作者: Tangprasertchai,NarinS;DiFelice,Rosa;Zhang,Xiaojun;Slaymaker,IanM;VazquezReyes,Carolina;Jiang,Wei;Rohs,Remo;Qin,PeterZ
• 通讯作者: Qin,PeterZ

Posttranscriptional site-directed spin labeling of large RNAs with an unnatural base pair system under non-denaturing conditions.

非变性条件下使用非天然碱基对系统对大 RNA 进行转录后定点自旋标记

• DOI: 10.1039/d0sc01717e
• 发表时间: 2020-09-21
• 期刊: Chemical science
• 影响因子: 8.4
• 作者: Wang Y;Kathiresan V;Chen Y;Hu Y;Jiang W;Bai G;Liu G;Qin PZ;Fang X
• 通讯作者: Fang X

Low temperature synthesis and characterization of lanthanide-doped BaTiO3 nanocrystals.

• DOI: 10.1039/c3cc49575b
• 发表时间: 2014-03
• 期刊: Chemical communications
• 影响因子: 4.9
• 作者: Sean P. Culver;V. Stepanov;Matthew Mecklenburg;Susumu Takahashi;R. Brutchey

Conformations of Human Telomeric G-Quadruplex Studied Using a Nucleotide-Independent Nitroxide Label.

• DOI: 10.1021/acs.biochem.5b01189
• 发表时间: 2016-01-19
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Zhang X;Xu CX;Di Felice R;Sponer J;Islam B;Stadlbauer P;Ding Y;Mao L;Mao ZW;Qin PZ
• 通讯作者: Qin PZ