Molecular Cytogenetics Shared Resource

分子细胞遗传学共享资源：项目总结 分子细胞遗传学共享资源（MCSR）提供先进的DNA修复和基因组 赫伯特欧文综合癌症中心（CIMCC）的成员进行不稳定性分析。的服务 包括（1）分析染色体结构、形态和重排模式（包括 核型分析、光谱核型分析（SKY）、染色体涂染和荧光原位杂交[FISH]）， (2)分析特定基因组区域（端粒，rDNA）的基因组不稳定性，（3）DNA损伤反应 和修复途径分析（包括DNA修复蛋白灶的动力学），（4）全基因组的发展 分析染色体易位和突变模式的工具（包括高通量测序） 平台），和（5）咨询和完成的实验设计，染色，数据采集包，和 处理.在DNA双链断裂修复专家查山博士的带领下， 全面和可定制的服务，以及由MCSR维护的专业仪器， 使研究人员在不同的技能水平与知识，以表征克隆变化以及非- 癌细胞和多种生物体的癌前组织中克隆基因组不稳定性。来 在当前项目期间（2014-2019年），MSR经历了领导和仪器重组。在这 在这一过程中，MCSR获得了新的设备并开发了新的检测方法，这大大扩展了其能力， 定量研究DNA修复机制。重组后的MCSR现在由一个专门的博士管理- 我们的员工水平，并提供方便，具有成本效益，创新的服务，以CNOCC的成员。MCSR 提供商业来源无法提供的服务，在纽约内不易获得 大都市区，并被证明是至关重要的研究，许多CIMCC成员，提供 赠款申请和高影响力出版物的关键初步数据。除了提供基本的和 在DNA修复和基因组不稳定性研究的不同领域的先进服务，MCSR还提供 向委员会成员提供专业仪器操作以及样品制备和分析方面的培训。 此外，还向调查人员提供咨询服务，协助设计最具成本效益的 分析并提供验证测定和数据所需的对照。MCSR致力于 结合新的实验组合和分析工具的使用，提供最先进的基因组 稳定服务，并通过培训和咨询促进服务的可及性， 合作和创新的研究正在进行中，在CIMCC。在本项目期间， 由21个国家信息和通信技术委员会成员使用，支持七个同行评审出版物的关键数据，包括四篇论文 在高影响力期刊（例如，Nature，Molecular Cell），目前支持五项NIH资助的研究。 项目，四个来自NCI。

MOLECULAR CYTOGENETICS SHARED RESOURCE: PROJECT SUMMARY The Molecular Cytogenetics Shared Resource (MCSR) provides access to advanced DNA repair and genomic instability analyses to members of the Herbert Irving Comprehensive Cancer Center (HICCC). The services include (1) analyses of chromosomal structural, morphological, and rearrangement patterns (including karyotyping, spectral karyotyping (SKY), chromosome painting, and fluorescence in situ hybridization [FISH]), (2) analyses of genomic instability at specialized genomic regions (telomere, rDNA), (3) DNA damage responses and repair pathway analyses (including the kinetics of DNA repair protein foci), (4) development of genome-wide tools to analyze chromosomal translocation and mutations patterns (including high throughput sequence platforms), and (5) consultation and completed packages for experimental design, staining, data acquisition, and processing. Under the leadership of Shan Zha, PhD, an expert in DNA double-strand breaks repair, these comprehensive and customizable services, together with specialized instruments maintained by the MCSR, enable investigators at varying skill levels with the knowledge to characterize clonal changes as well as non- clonal genomic instability in cancer cells and in premalignant tissues from a wide range of organisms. Over the current project period (2014-2019), MSR has undergone leadership and instrument reorganization. In this process, the MCSR acquired new equipment and developed new assays, which significantly expanded its ability to quantitatively study DNA repair mechanisms. The reorganized MCSR is now managed by a dedicated PhD- level staff and provides convenient, cost-effective, and innovative service to HICCC members. The MCSR provides services that are not available from commercial sources and not easily accessible within the New York metropolitan area and are proven to be critically important to the research of many HICCC members, providing key preliminary data for grant applications and high-impact publications. In addition to providing both basic and advanced services across different area of DNA repair and genomic instability studies, the MCSR also provides training to HICCC members in the operation of specialized instruments and in sample preparation and analyses. In addition, consulting services are available to investigators to assist in the design of the most cost-effective analyses and provide controls needed to validate the assay and the data. The MCSR is committed to incorporating novel experimental portfolios and the use of analytic tools, providing state-of-the-art genomic stability services, and promoting accessibility of services through training and consulting in support of highly collaborative and innovative research ongoing at the HICCC. During the current project period, the MCSR was utilized by 21 HICCC members, supported key data for seven peer-reviewed publications, including four papers in high-impact journals (e.g., Nature, Molecular Cell), and currently supports research for five NIH-funded projects, four from NCI.