Integrated microbiome analysis of the gut-blood-brain axis to delineate progression to Alzheimer's Disease

对肠-血-脑轴进行综合微生物组分析，以描绘阿尔茨海默病的进展

• 批准号: 10252549
• 负责人: Supriyo De
• 金额: $ 8.68万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10252549
• 关键词: Age; Alzheimer' s Disease; Alzheimer' s disease pathology; Alzheimer' s disease patient; Blood; Brain; Cells; DNA; Data; Dementia; Diffusion Magnetic Resonance Imaging; Elderly; Goals; Individual; Inflammation; Mitochondrial RNA; Neurodegenerative Disorders; Pathogenesis; Plasma; RNA; Sampling; Serum; epigenome; gastrointestinal; metagenomic sequencing; microbial; microbiome analysis; mild cognitive impairment; neuroinflammation; transcriptome; transcriptome sequencing

We performed RNA sequencing (RNA-seq) of the EVs in the serum of HC (Healthy donors), MCI (mild cognitive impairment) and AD (mild dementia due to Alzheimers disease) individuals with 110 bp paired-end directional reads. We observed significant differences in mitochondrial RNA abundance in EVs from AD/MCI/HC serum. We validated these changes using RT-qPCR analysis and obtained plasma samples from an additional 5 individuals per group and performed RT-qPCR analysis. In summary, in EVs of MCI and AD patients, we found the levels of mt-RNAs are robustly and significantly more abundant. We are currently working on the metagenomic sequencing and corelating our data with the results of DTI.

我们对HC（健康供体）、MCI（轻度认知障碍）和AD（由于阿尔茨海默病的轻度痴呆）个体的血清中的EV进行了RNA测序（RNA-seq），具有110 bp配对末端定向读段。我们观察到来自AD/MCI/HC血清的EV中线粒体RNA丰度的显著差异。我们使用RT-qPCR分析验证了这些变化，并从每组另外5名个体中获得血浆样本，并进行RT-qPCR分析。总之，在MCI和AD患者的EV中，我们发现MT-RNA的水平稳健且显著更丰富。我们目前正在进行宏基因组测序，并将我们的数据与DTI的结果相关联。