Flow Cytometry Core

项目摘要 流式细胞仪核心 流式细胞术核心（FCC）一直是莫菲特癌症中心支持的共享资源设施， 1990年，为莫菲特的癌症相关研究提供集中和尖端的流式细胞术服务。 会员可以随时方便地访问FCC，并在工作时间获得现场协助。的 联邦通信委员会的首要目标是为会员和学员提供高技能人员和最先进的技术支持 在高通量和多维流式细胞仪采集和细胞分选方面提供技术援助。FCC 支持这一目标的活动分为三个具体目标： 目标1：在实验设计和分析方面提供专家协助。 目标2：提供最先进的流式细胞术服务。 目标3：提供培训和教育。 FCC配备了一位知名的科学总监和免疫学专家Paulo C.罗德里格斯，和一个 由经验丰富的技术人员组成的团队，他们共同拥有超过65年的研究实践经验 在流式细胞术中。自上次竞争性更新以来，FCC扩大了其优势，并增加和升级了 它的仪器。目前的FCC服务包括：1）精确检测和分析多个参数， 单细胞水平; 2）快速纯化靶群体，评估特定细胞功能， 分子表征纯化的细胞群; 3）协助实验设计，数据分析和 口译;以及4）提供流式细胞术技术方面的专家培训和教育。 值得注意的是，FCC的支持为莫菲特的多个高影响力转化研究项目做出了贡献。来 在过去的五年里，FCC的使用量增加了51%，这导致了120篇同行评审的出版物，其中30篇是高质量的。 影响期刊。此外，FCC培训了130名研究人员和学员，并保持了一个优秀的成员 满意率-每年超过90%。在上一个财政年度（FY 20），FCC支持了四个国家的61名成员。 五个计划（CBE 25%，CE 3%，MM 36%，IO 36%），其中75%的成员持有同行评审 经费这占所有FCC使用量的98%。在下一个周期，FCC将继续支持所有CCSG 根据莫菲特会员日益增长的流式细胞术需求，计划并进一步扩大其服务。 未来FCC的优先事项是扩大其在多参数单细胞方面的技术和咨询服务 分析、高速细胞分选和大数据流式细胞术工作流程。为了满足这些需求，FCC 将获得一个新的快速细胞分选仪以及一个基于光谱的细胞仪，提供多达40+参数 流式细胞仪最后，公平竞争委员会将扩大其教育和培训举措的范围，并将制定一个流程， 流式细胞术兴趣小组，讨论流式细胞术的出版物、正在进行的项目和最新方法。

PROJECT SUMMARY FLOW CYTOMETRY CORE The Flow Cytometry Core (FCC) has been a Moffitt Cancer Center-supported Shared Resource Facility since 1990 and provides centralized and cutting-edge flow cytometry services for Moffitt’s cancer-related research. FCC is conveniently accessible to Members at all hours and with on-site assistance during working hours. The overarching goal of FCC is to support Members and trainees with highly skilled personnel and state-of-the-art technical assistance in high-throughput and multi-dimensional flow cytometry acquisition and cell sorting. FCC activities supporting this goal are organized intro three Specific Aims: Aim 1: To deliver expert assistance in experimental design and analysis. Aim 2: To provide state-of-the-art flow cytometry services. Aim 3: To provide training and education. FCC is staffed with a well-established Scientific Director and immunology expert, Dr. Paulo C. Rodriguez, and a team of highly experienced technologists, who collectively have more than 65 years of research-based practice in flow cytometry. Since the last competitive renewal, FCC expanded on its strengths, and added and upgraded its instrumentation. Current FCC services include: 1) Accurate detection and analysis of multiple parameters at the single-cell level; 2) Ability to rapidly purify target populations, to assess specific cellular functions, and to molecularly characterize purified cell populations; 3) Assistance in experimental design, data analysis and interpretation; and 4) Delivery of expert training and education in flow cytometry technologies. Notably, FCC support has contributed to multiple high impact translational research projects at Moffitt. Over the last five years, FCC usage increased by 51%, which resulted in 120 peer-reviewed publications, with 30 in high impact journals. Moreover, FCC trained 130 research staff and trainees, and maintained an excellent member satisfaction rate – over 90% – each year. Over the last fiscal year (FY20), FCC supported 61 Members in four of the five Programs (CBE 25%, CE 3%, MM 36%, IO 36%), with 75% of those Members holding peer review funding. This accounted for 98% of all FCC usage. In the next cycle, FCC will continue supporting all CCSG Programs and further expand its services based on the growing flow cytometry needs of Moffitt Members. Future FCC priorities are to expand its technical and consultation services in multi-parametric single-cell analysis, high-speed cell sorting, and big-data flow cytometry workflow processes. To address these needs, FCC will acquire a new fast-speed cell sorter as well as a spectral-based cytometer that provides up to 40+ parameter flow cytometry. Finally, FCC will expand its repertoire of education and training initiatives and will develop a Flow Cytometry Interest Group that discusses publications, ongoing projects, and latest approaches in flow cytometry.