Supplement application for a CLARIOstar Plus microplate reader with six detection modalities

补充摘要 具有六种检测模式的APLIOstar Plus酶标仪的补充申请 这是一份行政补充申请书，用于购买一个MPLIOstar Plus多模式微孔板 作者：BMG Labtech XIOStar是一款高性能、中等通量的仪器， 环境控制，提供了一个统一的解决方案，以六种检测方式，在我们的日常使用 NIGMS资助的工作：UV/维斯吸光度，荧光，发光，荧光偏振， 时间分辨荧光和ALPHA测定。我之所以要求使用AZIOStar，是因为以下原因。（一） 它将取代我实验室里用于Hedgehog和WNT的坏掉的、有十年历史的酶标仪 我的NIGMS MIRA（R35 GM 118082）奖支持的所有项目的核心信号分析。(2)它将 通过加强我们的研究，推进我在2021年NIGMS R35/MIRA更新中概述的研究计划的目标。 能够通过环境控制、蛋白质-蛋白质和小分子-蛋白质进行活细胞测定 相互作用测量和动力学测量，所有这些都具有十倍的速度，灵敏度和动态 与我们以前的仪器相比。(3)具有这些能力的酶标仪目前还不 在斯坦福大学提供的成本效益的基础上，考虑到我们的密集使用需求。生物化学系 斯坦福大学的一个研究所将用仪器服务和放置微孔板的空间的资金来支持这次购买 读者总而言之，该卫星将加速我实验室的NIGMS资助的研究项目， 为从事这些项目的学员提供检测设计和优化方面的尖端培训。

Supplement Summary Supplement application for a CLARIOstar Plus microplate reader with six detection modalities This is an administrative supplement application for the purchase of a CLARIOstar Plus multi-mode microplate reader from BMG Labtech. The CLARIOStar is a high-performance, medium-throughput instrument with environmental control that provides a unifying solution to the six detection modalities used routinely in our NIGMS-funded work: UV/Vis absorbance, fluorescence, luminescence, fluorescence polarization, time-resolved fluorescence and ALPHA assays. I am requesting the CLARIOStar for the following reasons. (1) It will replace a broken, decade-old microplate reader that is used in my laboratory for Hedgehog and WNT signaling assays central to all the projects supported by my NIGMS MIRA (R35 GM118082) award. (2) It will advance the goals of the research program outlined in my 2021 NIGMS R35/MIRA renewal by enhancing our capability to perform live cell assays with environmental control, protein-protein and small molecule-protein interaction measurements, and kinetic measurements, all with ten-fold higher speed, sensitivity and dynamic range compared to our previous instrument. (3) A microplate reader with these capabilities is not presently available at Stanford on a cost-effective basis given our intensive usage needs. The Biochemistry Department at Stanford will support this purchase with both funds for instrument service and space to house the microplate reader. In summary, the CLARIOstar will accelerate NIGMS-funded research projects in my laboratory and provide cutting-edge training in assay design and optimization for trainees that work on these projects.