Resource for Multiphoton Characterization of Genetically-Encoded Probes

遗传编码探针的多光子表征资源

• 批准号: 10583530
• 负责人: Mikhail Drobizhev
• 金额: $ 24.32万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-30 至 2024-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10583530
• 关键词: BRAIN initiative; Biliverdine; Binding; Biosensor; Cells; Cyclic AMP; Dependence; Educational workshop; Equilibrium; Equipment; Extinction; Feedback; Fluorescence; Goals; Harvest; Jellyfish; Label; Laboratories; Laser Scanning Microscopy; Lasers; Ligands; Measurement; Measures; Methods; Microscopy; Mission; Molecular; Montana; National Institute of Neurological Disorders and Stroke; Nature; Neurotransmitters; Optics; Photobleaching; Photons; Phytochrome; Process; Property; Protein Engineering; Proteins; Publications; Reference Standards; Research; Research Personnel; Resources; Sampling; Second Messenger Systems; Services; Signal Transduction; Training; United States National Institutes of Health; Universities; Work; absorption; chromophore; coral; extracellular; improved; multi-photon; neural network; neuronal circuitry; quantum; response; sensor; tool; two-photon; virtual; web site

Two-photon laser scanning microscopy powers many projects in the. BRAIN initiative because it is the best way to capture the concerted activity of living neural networks. Genetically-encoded activity probes and sensors built with fluorescent proteins are the tools of choice for labeling and recording from living circuits of neurons. However, to date, virtually all optimization of these probes has been solely in the one-photon regime. This is mainly because measuring quantitative two-photon absorption properties demands more sophisticated equipment and expertise in photophysics and nonlinear optics. Over the past five years, our group has informally provided this service for protein engineers and neuroscientists: they sent us newly developed proteins and we carefully characterized their 2PA properties. While this has worked in the past for just a few laboratories, the BRAIN initiative has fueled many more laboratories in need of this service to the extent that we now need support to establish a Resource. In this project, we will establish a unique Resource for Multiphoton Characterization of Genetically-Encoded Probes at the Montana State University Campus (Bozeman, MT) that will continue providing the service of characterization of 2PA properties and multiphoton stability to a much large number of protein engineers and neuroscientists. We are planning to provide a very broad access to our facility for Investigators whose research is relevant to the NIH/NINDS mission and to the goals of the BRAIN initiative. The information obtained will be broadly disseminated via scientific publications and one of the microscopy websites. We will organize annual workshops where we will provide training on 2PA photophysics and methods of measurements and provide all necessary information for researchers who were willing to start such measurements in their labs.

双光子激光扫描显微镜为许多项目提供动力。大脑主动