Mass Spectrometry

项目摘要：质谱学核心(前身为代谢组学核心) 糖尿病及其相关的内分泌和代谢紊乱的特点是 动态平衡。异常信号级联涉及蛋白质翻译后修饰，如 磷酸化、潜在毒性脂质物种的积累和细胞内代谢产物的流量改变是 在构成糖尿病环境的异常中。DRC质谱学核心，一个扩展的 以前的代谢组学核心的版本，提供严格的基于质谱学的蛋白质组学和 向DRC研究人员提供的代谢组学分析，包括量化、结构特征和 糖尿病相关生物分子的功能表征。核心提高了效率和成本 通过提供集中、标准化的质谱分析来研究分子的有效性 糖尿病的发病机制、危险因素和并发症。世界银行的一个主要目标 核心是通过培训促进在糖尿病研究中使用质谱学(MS)方法， 协作、开发、服务和传播。核心的具体目标是：1)提供和 为糖尿病相关研究维护正常的MS系统；2)就以下问题向刚果民主共和国调查人员提供咨询 MS如何推进他们的研究计划；3)对糖尿病进行与服务相关的MS分析 研究人员，如量化目标分析物，获得用于结构鉴定的光谱，以及协助 4)开发新的MS方法；5)为学生提供培训和 MS系统原理和使用方面的研究员。在过去的资助期内，这个核心作为 代谢组学核心，为34个不同的刚果民主共和国成员实验室提供服务，包括3个P&F接受者， 而11,000小时的核心服务支持了90篇论文的发表。通过大量的 机构投资，这个核心的能力已经增加，促使更名为刚果民主共和国质量 光谱分析核心。质谱学核心提供的服务反映了不断发展的生物分析 刚果民主共和国调查人员的需求，包括1)蛋白质表达的定量研究，包括靶向分析 来自临床样本；2)蛋白质相互作用的定量评估；3)POST的特征 翻译修饰；4)阐明蛋白质或蛋白质组的生物学功能；5)有针对性 代谢组学服务；6)对多种代谢途径的广泛调查和途径的量化 通过非靶向代谢组学获得代谢物；以及7)分析物的高通量、质量控制测量 在来自临床研究的大样本集合中。因此，质谱学核心利用了大量的机构 对仪器和分析专门知识的投资，以促进新发现和科学进步 华盛顿大学DRC的糖尿病研究。

PROJECT SUMMARY: Mass Spectrometry Core (formerly Metabolomics Core) Diabetes and its related endocrine and metabolic disorders are characterized by disruption of homeostasis. Aberrant signaling cascades involving protein post translational modifications such as phosphorylation, accumulation of potentially toxic lipid species, and altered flux of intracellular metabolites are among the abnormalities that constitute the diabetic milieu. The DRC Mass Spectrometry Core, an expanded version of the former Metabolomics Core, provides rigorous mass spectrometry-based proteomics and metabolomics analyses to DRC investigators that include quantification, structural characterization, and functional characterization of diabetes-related biomolecules. The Core increases efficiency and cost effectiveness by providing centralized, standardized mass spectrometry analyses to study molecular mechanisms of the pathogenesis of diabetes, its risk factors, and its complications. A major goal of the core is to promote use of mass spectrometry (MS) methods in diabetes research through training, collaboration, development, service, and dissemination. Specific objectives of the Core are: 1) to provide and maintain functional MS systems for diabetes-related studies; 2) to consult with DRC investigators regarding how MS can advance their research programs; 3) to perform service-related MS analyses for diabetes investigators, such as quantifying target analytes, obtaining spectra for structural identification, and assisting with mass spectra interpretation; 4) to develop new MS methods; and 5) to provide training to students and fellows in principles and use of MS systems. During this past funding period, this Core, operating as the Metabolomics Core, provided services to 34 different DRC member laboratories, including 3 P&F recipients, and the >11,000 hours of Core service supported the publication of 90 papers. Through a substantial institutional investment, the capabilities of this Core have increased prompting a name change to DRC Mass Spectrometry Core. The services offered by the Mass Spectrometry Core reflect the evolving bioanalytical needs of DRC investigators, including 1) quantitative study of protein expression, including targeted assays from clinical samples; 2) quantitative assessment of protein-protein interactions; 3) characterization of post translational modifications; 4) elucidation of the biological functions of proteins or protein groups; 5) targeted metabolomic services; 6) broad surveys of multiple metabolic pathways and quantification of pathway metabolites via untargeted metabolomics; and 7) high-throughput, quality-controlled measurements of analytes in large sample sets from clinical studies. The Mass Spectrometry Core thus leverages substantial institutional investments in instrumentation and analytical expertise to promote new discoveries and scientific progress in diabetes research at the Washington University DRC.