Mass Spectrometry

项目概要：质谱核心（原代谢组学核心） 糖尿病及其相关的内分泌和代谢紊乱的特征是破坏了 体内平衡涉及蛋白质翻译后修饰的异常信号级联， 磷酸化、潜在毒性脂质物质的积累和细胞内代谢物的通量改变是 构成糖尿病环境的异常之一。DRC质谱核心，扩展的 前代谢组学核心的版本，提供了严格的基于质谱的蛋白质组学， 向DRC研究人员提供代谢组学分析，包括定量、结构表征和 糖尿病相关生物分子的功能表征。核心提高了效率和成本 通过提供集中的、标准化的质谱分析来研究分子 糖尿病的发病机制、危险因素及其并发症。的一个主要目标 核心是通过培训促进质谱（MS）方法在糖尿病研究中的使用， 协作、发展、服务和传播。核心的具体目标是：1）提供和 维持糖尿病相关研究的功能MS系统; 2）与DRC研究者就以下问题进行咨询 MS如何推进其研究计划; 3）进行与服务相关的MS糖尿病分析 研究人员，如定量目标分析物，获得光谱结构鉴定，并协助 质谱解释; 4）开发新的MS方法; 5）为学生提供培训， 研究员的原则和MS系统的使用。在过去的供资期间，该核心作为 Metabolomics Core为34个不同的DRC成员实验室提供服务，包括3个P&F接受者， 超过11，000小时的核心服务支持了90篇论文的发表。通过大幅度 机构投资，这一核心的能力已经增加，促使名称改为DRC质量 光谱核心。质谱核心提供的服务反映了不断发展的生物分析 DRC研究人员的需求，包括1）蛋白质表达的定量研究，包括靶向测定 从临床样品; 2）蛋白质-蛋白质相互作用的定量评估; 3）后 翻译修饰; 4）阐明蛋白质或蛋白质组的生物学功能; 5）靶向 代谢组学服务; 6）多种代谢途径的广泛调查和途径的定量 通过非靶向代谢组学的代谢物;和7）分析物的高通量、质量控制的测量 在临床研究的大样本集中。因此，质谱核心利用了大量的机构 投资于仪器和分析专门知识，以促进新发现和科学进步， 华盛顿大学DRC的糖尿病研究。

PROJECT SUMMARY: Mass Spectrometry Core (formerly Metabolomics Core) Diabetes and its related endocrine and metabolic disorders are characterized by disruption of homeostasis. Aberrant signaling cascades involving protein post translational modifications such as phosphorylation, accumulation of potentially toxic lipid species, and altered flux of intracellular metabolites are among the abnormalities that constitute the diabetic milieu. The DRC Mass Spectrometry Core, an expanded version of the former Metabolomics Core, provides rigorous mass spectrometry-based proteomics and metabolomics analyses to DRC investigators that include quantification, structural characterization, and functional characterization of diabetes-related biomolecules. The Core increases efficiency and cost effectiveness by providing centralized, standardized mass spectrometry analyses to study molecular mechanisms of the pathogenesis of diabetes, its risk factors, and its complications. A major goal of the core is to promote use of mass spectrometry (MS) methods in diabetes research through training, collaboration, development, service, and dissemination. Specific objectives of the Core are: 1) to provide and maintain functional MS systems for diabetes-related studies; 2) to consult with DRC investigators regarding how MS can advance their research programs; 3) to perform service-related MS analyses for diabetes investigators, such as quantifying target analytes, obtaining spectra for structural identification, and assisting with mass spectra interpretation; 4) to develop new MS methods; and 5) to provide training to students and fellows in principles and use of MS systems. During this past funding period, this Core, operating as the Metabolomics Core, provided services to 34 different DRC member laboratories, including 3 P&F recipients, and the >11,000 hours of Core service supported the publication of 90 papers. Through a substantial institutional investment, the capabilities of this Core have increased prompting a name change to DRC Mass Spectrometry Core. The services offered by the Mass Spectrometry Core reflect the evolving bioanalytical needs of DRC investigators, including 1) quantitative study of protein expression, including targeted assays from clinical samples; 2) quantitative assessment of protein-protein interactions; 3) characterization of post translational modifications; 4) elucidation of the biological functions of proteins or protein groups; 5) targeted metabolomic services; 6) broad surveys of multiple metabolic pathways and quantification of pathway metabolites via untargeted metabolomics; and 7) high-throughput, quality-controlled measurements of analytes in large sample sets from clinical studies. The Mass Spectrometry Core thus leverages substantial institutional investments in instrumentation and analytical expertise to promote new discoveries and scientific progress in diabetes research at the Washington University DRC.