Core B Ocular Imaging Core

核心 B 眼部成像核心

• 批准号: 10273078
• 负责人: VERA L BONILHA
• 金额: $ 27.47万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10273078
• 关键词: Animal Model; Animals; Center Core Grants; Clinic; Collaborations; Computer software; Confocal Microscopy; Cryoultramicrotomy; Data; Disease Progression; Equipment; Eye; Faculty Recruitment; Fluorescence; Fluorescence Microscopy; Fostering; Frozen Sections; Funding; Fundus photography; Generations; Goals; Grant; Image; Image Analysis; Imaging Techniques; Immunohistochemistry; Lasers; Mind; Mus; Ophthalmoscopy; Optical Coherence Tomography; Oryctolagus cuniculus; Paraffin; Paraffin Embedding; Pathway interactions; Plastic Embedding; Process; Productivity; Protocols documentation; Rattus; Research; Research Personnel; Research Support; Resources; Scanning; Scientist; Services; Stains; Standardization; Supervision; Talents; Techniques; Technology; Testing; Tissue Embedding; Tissue Fixation; Tissue imaging; Tissues; Training; Translational Research; Transmission Electron Microscopy; United States National Institutes of Health; Vision; Vision research; Visual system structure; Zebrafish; animal imaging; cellular imaging; clinical imaging; ex vivo imaging; experience; faculty research; human imaging; image processing; imaging modality; innovation; instrument; instrumentation; machine learning algorithm; microscopic imaging; ocular imaging; programs; ranpirnase; recruit; sample fixation; tissue processing; vision science; visual tracking

Abstract Ocular Imaging Core The Ocular Imaging Core will provide a variety of imaging modalities to the investigators involved in this P30 Cole Eye Vision Research Core. The module includes live animal (in vivo) imaging with slit lamp and fundus photography, scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT). It also supports tissue and cellular imaging (ex vivo) support, providing training and assistance with routine and specialized tissue fixation techniques, paraffin and plastic tissue embedding, sectioning (paraffin and Cryo) and staining for immunohistochemistry. Microscope imaging techniques include bright field, dark field, differential interference contrast (DIC), fluorescence, and confocal microscopy . It also provides help and advice with the morphometric analysis of generated images. Access to this core module will support NEI R01 funded investigators, help newly recruited investigators obtain data for NEI R01 grant submissions, foster collaborative interactions between RO1-funded investigators and newly recruited investigators, attract investigators from other departments to research on the visual system and be a valuable departmental resource in attracting additional vision scientists to our program as we expand our research faculty.

摘要 眼部成像核心 眼部成像核心将为参与该P30科尔眼的研究者提供各种成像模式 视觉研究核心。该模块包括活体动物（活体）裂隙灯成像和眼底摄影， 扫描激光检眼镜（SLO）和光学相干断层扫描（OCT）。它还支持组织和细胞 成像（离体）支持，提供常规和专业组织固定技术的培训和帮助， 石蜡和塑料组织包埋，切片（石蜡和冷冻）和免疫组织化学染色。 显微镜成像技术包括明场、暗场、微分干涉对比（DIC）、荧光、 和共聚焦显微镜。它还为生成图像的形态分析提供帮助和建议。接入 该核心模块将支持NEI R01资助的研究者，帮助新招募的研究者获得NEI数据 R01赠款提交，促进R01资助的调查员和新征聘的调查员之间的协作互动 研究人员，吸引其他部门的研究人员对视觉系统的研究，并成为一个有价值的 部门资源，吸引更多的视觉科学家到我们的计划，因为我们扩大我们的研究人员。