Development of a Microdevice for Photoelectrical Stimulation of Cells

细胞光电刺激微型装置的开发

• 批准号: 10263886
• 负责人: Jingjiao Guan
• 金额: $ 7.1万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10263886
• 关键词: Acids; Alkanesulfonates; Allylamine; Astrocytes; Atomic Force Microscopy; Behavior; Binding; Biological Assay; Bovine Serum Albumin; Brain; Butyric Acids; Calcium ion; Caliber; Cell Differentiation process; Cell Proliferation; Cell membrane; Cell surface; Cells; Cultured Cells; Development; Devices; Dimensions; Dyes; Electric Stimulation; Electrons; Engineering; Ensure; Esters; Fluorescence; Foundations; Generations; Glutaral; Gold; Hippocampus (Brain); Human; Implanted Electrodes; In Vitro; Individual; Ion Channel; Light; Lighting; Literature; Measures; Microprocessor; Microscopy; Modeling; Mus; Nerve Tissue; Neurons; Optics; Organoids; Pattern; Performance; Physiologic pulse; Polymers; Protocols documentation; Research; Resolution; Scanning Electron Microscopy; Shapes; Signal Transduction; Structure; Styrenes; Surface; Surface Properties; Techniques; Thick; Time; Tissue Engineering; Tissues; Titanium; base; cell type; design; effective therapy; flexibility; microdevice; nervous system disorder; neuroblastoma cell; neuroregulation; novel; novel therapeutics; programs; titanium dioxide; tool; voltage

PROJECT SUMMARY/ABSTRACT Photoelectrical stimulation of cells is an emerging technique with tremendous potential for expanding the toolset for tissue engineering as well as enabling new therapies for treating neurological diseases, but its potential applications are limited by the large size of the existing photoelectrical-stimulation devices. The objective of this proposed study is to develop a novel device for photoelectrical stimulation. The device, which is termed microdevice, is featured by a sub-10 µm size and disk-like shape, a well-defined layered structure for converting light to electrical signals via the photovoltaic mechanism, and engineerable surface properties. These features potentially allow the microdevices to bind to individual cells and modulate their behaviors through photoelectrical stimulation. The specific aims are to (1) establish a protocol for fabricating the designed microdevices, and (2) evaluate the performance of the microdevices in photoelectrical stimulation of cells. Successful completion of the proposed research will validate feasibility of the microdevice and lay a foundation for its further development into a viable tool for tissue engineering research and neuromodulation therapy.

项目摘要/摘要 细胞的光电刺激是一种新兴的技术，具有巨大的潜力 用于组织工程的工具包，以及实现治疗神经疾病的新疗法，但其 现有的光电刺激设备尺寸太大，限制了其潜在的应用。这个 本研究的目的是开发一种新型的光电刺激装置。该设备，这是 称为微器件，其特征是小于10微米的尺寸和盘状形状，定义良好的分层结构 通过光伏机制将光转换为电信号，以及可工程的表面特性。这些 这些特征潜在地允许微型设备绑定到单个细胞，并通过 光电刺激。其具体目标是(1)建立用于制造所设计的 以及(2)评估微器件在光电刺激细胞方面的性能。 拟议研究的成功完成将验证微器件的可行性，并为其奠定基础 为其进一步发展成为组织工程研究和神经调节治疗的可行工具。