One Health Framework for Investigating Antimicrobial Resistant Enterobacterales Population Dynamics in Southwestern Virginia by Whole Genome Sequencing

通过全基因组测序研究弗吉尼亚州西南部耐药肠杆菌种群动态的一个健康框架

• 批准号: 10610265
• 负责人: KEVIN K LAHMERS
• 金额: $ 3.83万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10610265
• 关键词: Health; Framework; Investigating; Antimicrobial; Resistant

Project Summary Antimicrobial resistance is a major threat to public health worldwide. The Centers for Disease Control and Prevention has identified extended spectrum beta lactamase-producing Enterobacterales (ESBL-E) as a serious threat to public health, and these organisms cause clinical infections in humans and a variety of veterinary species, including dogs and cats. Therefore, it is essential to adopt a “One Health” approach to understand and combat antimicrobial resistance. In addition, fecal carriage of the same strain of ESBL-E. coli has been demonstrated to be shared between humans and pets in the same household. However, the relationship between clinical isolates of ESBL-E isolated from infections in pets and people on a population level is unclear. The aims of this study are to: 1) expand capacity of Virginia Tech Animal Laboratory Services to perform whole genome sequencing and sequence analysis, and 2) determine the relatedness of ESBL-E isolated from both of human and companion animal infections isolated over a similar time-period and in the same geographic locations by genome sequence analysis. We have identified 60 clinical ESBL-E isolated between 2018 and 2021 from humans and companion animals in southwestern Virginia and have partially characterized those isolates by identifying ESBL genes. We propose to perform short-read whole genome sequencing (WGS) on those isolates at Virginia Tech Animal Laboratory Services in order to improve our laboratory’s capacity to perform and interpret WGS. Sequencing data will be evaluated for resistance gene and multilocus sequence (MLST) analysis. Isolates of the same MSLT type will undergo single-nucleotide polymorphism analysis to determine the relatedness of the organisms. Monitoring of regionally-specific population dynamics of drug-resistant pathogens allows for implementation of locally-relevant mitigation strategies that improve animal and human health, thereby directly supporting the Vet-LIRN mission to safeguard human and animal health and to increase our laboratory’s capacity to respond to Vet-LIRN investigations and enhance the safety of human and animal foods.

项目摘要 抗菌素耐药性是对全球公共健康的重大威胁。美国疾病控制中心 控制和预防中心发现产超广谱β-内酰胺酶 肠杆菌属(ESBL-E)作为严重威胁公众健康的一种细菌，并可引起 人类和包括狗和猫在内的各种兽医物种的临床感染。 因此，采取“一个健康”的方法来理解和打击是至关重要的 抗菌素耐药性。此外，同一菌株ESBL-E的粪便携带情况也不同。Coli已经被 展示了在同一个家庭中的人和宠物之间共享的。然而， 从宠物感染中分离的ESBL-E临床分离株与人群的关系 人口水平尚不清楚。这项研究的目的是：1)扩大弗吉尼亚理工大学的产能 动物实验室服务进行全基因组测序和序列分析，以及 2)确定从人和同伴动物中分离的ESBL-E的亲缘关系 按基因组分析在相似时间段和相同地理位置分离的感染 序列分析。我们在2018年至2021年期间从 弗吉尼亚州西南部的人类和同伴动物，并部分地描述了这些 通过鉴定ESBL基因进行分离。我们建议进行短读全基因组测序 (WGS)在弗吉尼亚理工大学动物实验室服务中心的这些分离物上，以改善我们的 实验室执行和解释WGS的能力。将对测序数据进行评估 抗性基因和多位点序列(MLST)分析。相同MSLT类型的分离物将 进行单核苷酸多态分析，以确定生物的亲缘关系。 监测耐药病原体的区域特定种群动态可以 实施与当地相关的缓解战略，以改善动物和人类健康； 从而直接支持兽医-LIRN任务，以保障人类和动物健康并 提高实验室应对VET-LIRN调查的能力，增强安全性 人类和动物性食品。