Inter-regional study of transmission, adaptation and pathogenesis of viruses with pandemic potential in Southeast Asia and West/Central Africa

对东南亚和西非/中非具有大流行潜力的病毒的传播、适应和发病机制进行区域间研究

• 批准号: 10626183
• 负责人: Anavaj SAKUNTABHAI
• 金额: $ 14.74万
• 依托单位: INSTITUT PASTEUR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-17 至 2025-10-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10626183
• 关键词: Amblyomma; Antibodies; Area; Asia; Bacteria; Behavior; Biological; Biological Assay; Biological Markers; Borrelia; Buffaloes; Cambodia; Cambodian; Canis familiaris; Castor Bean Tick; Cattle; Central Africa; Collection; Communities; Country; Coxiella; Crimean-Congo Hemorrhagic Fever Virus; Data; Dengue; Diagnosis; Documentation; Ehrlichia; Enzyme-Linked Immunosorbent Assay; Exposure to; Felis catus; France; Funding; Habits; Household; Human; Immunoglobulin G; Knowledge; Light; Location; Measures; Metagenomics; Methods; Molecular; Morphology; National Institute of Allergy and Infectious Disease; Outcome; Parents; Paris, France; Patients; Persons; Population; Prevalence; Protocols documentation; Province; Reporting; Rickettsia; Risk; Rural; Rural Community; Sampling; Sensitivity and Specificity; Seroprevalences; Severe Fever with Thrombocytopenia Syndrome Virus; Southeastern Asia; Specificity; Specimen; Testing; Tick-Borne Diseases; Tick-Borne Encephalitis Virus; Ticks; Time; United States National Institutes of Health; Viral Pathogenesis; Virus; calreticulin; candidate marker; efficacy evaluation; emerging pathogen; high risk; metagenomic sequencing; neglect; next generation sequencing; pandemic disease; pathogen; recombinant peptide; rural area; tick bite; tick saliva; tick-borne; tick-borne pathogen; tick-borne virus; transmission process; vector

PROJECT SUMMARY Background: In Cambodia, ticks and tick-borne diseases are neglected and data are still lacking. Objectives: In the present proposal, we aim to identify tick species and associated pathogens including virus and bacteria circulating in rural Cambodia. Additionally, we plan to investigate transmission risk of a number of selected tick- borne pathogens known to circulate in the region in human by assessing seroprevalence (IgG) and tick bite biomarkers in people living in the communities where ticks are captured. Methods: Our study will be nestled in a study, namely “Study of dengue-like illness in Kampong Thom Province, Cambodia” (DENTHOM) which is part of a NIH/NIAID-funded project, namely “Inter-regional study of transmission, adaptation and pathogenesis of viruses with pandemic potential in Southeast Asia and West/Central Africa” (U01 AI151758-01). Tick collections will be planned according to the parent study (DENTHOM). In terms of human samples, the present study involves only biospecimen collected in the framework of the DENTHOM study. No collection of human biological specimens will be specifically performed for the present proposal. The collected tick specimens will be first identified morphologically and confirmed using Maldi-TOF MS. Once identified, the specimen will be killed and kept at -80°C. Ticks samples will be tested for known viruses and bacteria using molecular assays and for novel pathogens using metagenomics sequencing. Human samples will be used for seroprevalence study of commonly known tick-borne viruses and bacteria circulating in Asia and for tick bite biomarkers. Expected outcomes: Our study will provide current state of knowledge of ticks and potential risks of tick-associated pathogens circulating in Cambodia. The findings of our study would be useful for assessment of host/vector interaction and instruct diagnosis of tick-borne pathogens.

项目摘要 背景：在柬埔寨，蜱虫和蜱传疾病被忽视，数据仍然缺乏。目的： 在目前的建议中，我们的目的是识别蜱种类和相关病原体，包括病毒和细菌 在柬埔寨农村流传。此外，我们计划调查一些选定的蜱虫的传播风险- 通过评估血清阳性率（IgG）和蜱叮咬，已知在该地区人类中传播的病原体 生物标志物在生活在蜱虫被捕获的社区的人们身上。方法：我们的研究将坐落在 一项名为“柬埔寨甘榜通省登革热样疾病研究”的研究， 美国国立卫生研究院/NIAID资助的一个项目，即“区域间研究的传播，适应和发病机制， 在东南亚和西非/中非具有大流行潜力的病毒”（U 01 AI 151758 -01）。蜱虫采集 将根据母研究（DENTHOM）进行计划。在人类样本方面，本研究 仅涉及在DENTHOM研究框架内收集的生物标本。没有收集人类生物 将专门为本提案制作标本。收集的蜱虫标本将首先 进行形态学鉴定并使用Maldi-TOF MS进行确认。一旦鉴定，将处死标本， 保持在-80 ° C。将使用分子测定法检测蜱样本中的已知病毒和细菌，并检测新的 使用宏基因组测序的病原体。人类样本将用于血清阳性率研究， 已知的蜱传病毒和在亚洲传播的细菌以及蜱叮咬生物标志物。预期成果： 研究将提供蜱虫知识的现状和蜱虫相关病原体传播的潜在风险 在柬埔寨。本研究的结果将有助于评估宿主/媒介相互作用和指导 蜱传病原体的诊断。