Inter-regional study of transmission, adaptation and pathogenesis of viruses with pandemic potential in Southeast Asia and West/Central Africa

对东南亚和西非/中非具有大流行潜力的病毒的传播、适应和发病机制进行区域间研究

• 批准号: 10626183
• 负责人: Anavaj SAKUNTABHAI
• 金额: $ 14.74万
• 依托单位: INSTITUT PASTEUR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-17 至 2025-10-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10626183
• 关键词: Amblyomma; Antibodies; Area; Asia; Bacteria; Behavior; Biological; Biological Assay; Biological Markers; Borrelia; Buffaloes; Cambodia; Cambodian; Canis familiaris; Castor Bean Tick; Cattle; Central Africa; Collection; Communities; Country; Coxiella; Crimean-Congo Hemorrhagic Fever Virus; Data; Dengue; Diagnosis; Documentation; Ehrlichia; Enzyme-Linked Immunosorbent Assay; Exposure to; Felis catus; France; Funding; Habits; Household; Human; Immunoglobulin G; Knowledge; Light; Location; Measures; Metagenomics; Methods; Molecular; Morphology; National Institute of Allergy and Infectious Disease; Outcome; Parents; Paris, France; Patients; Persons; Population; Prevalence; Protocols documentation; Province; Reporting; Rickettsia; Risk; Rural; Rural Community; Sampling; Sensitivity and Specificity; Seroprevalences; Severe Fever with Thrombocytopenia Syndrome Virus; Southeastern Asia; Specificity; Specimen; Testing; Tick-Borne Diseases; Tick-Borne Encephalitis Virus; Ticks; Time; United States National Institutes of Health; Viral Pathogenesis; Virus; calreticulin; candidate marker; efficacy evaluation; emerging pathogen; high risk; metagenomic sequencing; neglect; next generation sequencing; pandemic disease; pathogen; recombinant peptide; rural area; tick bite; tick saliva; tick-borne; tick-borne pathogen; tick-borne virus; transmission process; vector

PROJECT SUMMARY Background: In Cambodia, ticks and tick-borne diseases are neglected and data are still lacking. Objectives: In the present proposal, we aim to identify tick species and associated pathogens including virus and bacteria circulating in rural Cambodia. Additionally, we plan to investigate transmission risk of a number of selected tick- borne pathogens known to circulate in the region in human by assessing seroprevalence (IgG) and tick bite biomarkers in people living in the communities where ticks are captured. Methods: Our study will be nestled in a study, namely “Study of dengue-like illness in Kampong Thom Province, Cambodia” (DENTHOM) which is part of a NIH/NIAID-funded project, namely “Inter-regional study of transmission, adaptation and pathogenesis of viruses with pandemic potential in Southeast Asia and West/Central Africa” (U01 AI151758-01). Tick collections will be planned according to the parent study (DENTHOM). In terms of human samples, the present study involves only biospecimen collected in the framework of the DENTHOM study. No collection of human biological specimens will be specifically performed for the present proposal. The collected tick specimens will be first identified morphologically and confirmed using Maldi-TOF MS. Once identified, the specimen will be killed and kept at -80°C. Ticks samples will be tested for known viruses and bacteria using molecular assays and for novel pathogens using metagenomics sequencing. Human samples will be used for seroprevalence study of commonly known tick-borne viruses and bacteria circulating in Asia and for tick bite biomarkers. Expected outcomes: Our study will provide current state of knowledge of ticks and potential risks of tick-associated pathogens circulating in Cambodia. The findings of our study would be useful for assessment of host/vector interaction and instruct diagnosis of tick-borne pathogens.

项目概要 背景：在柬埔寨，蜱虫和蜱传疾病被忽视，并且仍然缺乏数据。目标： 在本提案中，我们的目标是识别蜱虫种类和相关病原体，包括病毒和细菌 流传于柬埔寨农村。此外，我们计划调查一些选定的蜱虫的传播风险 通过评估血清阳性率 (IgG) 和蜱虫叮咬，已知在该地区人类中传播的携带病原体 生活在蜱虫捕获社区的人们的生物标志物。方法：我们的研究将坐落在 一项研究，即“柬埔寨磅同省登革热样疾病研究”(DENTHOM)，该研究是 NIH/NIAID 资助的项目，即“传播、适应和发病机制的区域间研究” 在东南亚和西非/中非具有大流行潜力的病毒”（U01 AI151758-01）。蜱虫集合 将根据家长研究 (DENTHOM) 进行计划。就人类样本而言，本研究 仅涉及在 DENTHOM 研究框架内收集的生物样本。没有收集人类生物 样品将专门针对本提案进行。采集到的蜱虫标本将首先 进行形态学鉴定并使用 Maldi-TOF MS 进行确认。一旦确定，该样本将被杀死并 保存在-80°C。蜱虫样本将使用分子测定法检测已知病毒和细菌，并检测新病毒和细菌。 使用宏基因组测序来识别病原体。人体样本将用于常见疾病的血清阳性率研究 已知在亚洲传播的蜱传病毒和细菌以及蜱叮咬生物标志物。预期成果：我们的 研究将提供蜱虫知识的现状以及蜱虫相关病原体传播的潜在风险 在柬埔寨。我们的研究结果将有助于评估宿主/载体相互作用并指导 蜱传病原体的诊断。