Inter-regional study of transmission, adaptation and pathogenesis of viruses with pandemic potential in Southeast Asia and West/Central Africa

对东南亚和西非/中非具有大流行潜力的病毒的传播、适应和发病机制进行区域间研究

• 批准号: 10626183
• 负责人: Anavaj SAKUNTABHAI
• 金额: $ 14.74万
• 依托单位: INSTITUT PASTEUR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-17 至 2025-10-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10626183
• 关键词: Amblyomma; Antibodies; Area; Asia; Bacteria; Behavior; Biological; Biological Assay; Biological Markers; Borrelia; Buffaloes; Cambodia; Cambodian; Canis familiaris; Castor Bean Tick; Cattle; Central Africa; Collection; Communities; Country; Coxiella; Crimean-Congo Hemorrhagic Fever Virus; Data; Dengue; Diagnosis; Documentation; Ehrlichia; Enzyme-Linked Immunosorbent Assay; Exposure to; Felis catus; France; Funding; Habits; Household; Human; Immunoglobulin G; Knowledge; Light; Location; Measures; Metagenomics; Methods; Molecular; Morphology; National Institute of Allergy and Infectious Disease; Outcome; Parents; Paris, France; Patients; Persons; Population; Prevalence; Protocols documentation; Province; Reporting; Rickettsia; Risk; Rural; Rural Community; Sampling; Sensitivity and Specificity; Seroprevalences; Severe Fever with Thrombocytopenia Syndrome Virus; Southeastern Asia; Specificity; Specimen; Testing; Tick-Borne Diseases; Tick-Borne Encephalitis Virus; Ticks; Time; United States National Institutes of Health; Viral Pathogenesis; Virus; calreticulin; candidate marker; efficacy evaluation; emerging pathogen; high risk; metagenomic sequencing; neglect; next generation sequencing; pandemic disease; pathogen; recombinant peptide; rural area; tick bite; tick saliva; tick-borne; tick-borne pathogen; tick-borne virus; transmission process; vector

PROJECT SUMMARY Background: In Cambodia, ticks and tick-borne diseases are neglected and data are still lacking. Objectives: In the present proposal, we aim to identify tick species and associated pathogens including virus and bacteria circulating in rural Cambodia. Additionally, we plan to investigate transmission risk of a number of selected tick- borne pathogens known to circulate in the region in human by assessing seroprevalence (IgG) and tick bite biomarkers in people living in the communities where ticks are captured. Methods: Our study will be nestled in a study, namely “Study of dengue-like illness in Kampong Thom Province, Cambodia” (DENTHOM) which is part of a NIH/NIAID-funded project, namely “Inter-regional study of transmission, adaptation and pathogenesis of viruses with pandemic potential in Southeast Asia and West/Central Africa” (U01 AI151758-01). Tick collections will be planned according to the parent study (DENTHOM). In terms of human samples, the present study involves only biospecimen collected in the framework of the DENTHOM study. No collection of human biological specimens will be specifically performed for the present proposal. The collected tick specimens will be first identified morphologically and confirmed using Maldi-TOF MS. Once identified, the specimen will be killed and kept at -80°C. Ticks samples will be tested for known viruses and bacteria using molecular assays and for novel pathogens using metagenomics sequencing. Human samples will be used for seroprevalence study of commonly known tick-borne viruses and bacteria circulating in Asia and for tick bite biomarkers. Expected outcomes: Our study will provide current state of knowledge of ticks and potential risks of tick-associated pathogens circulating in Cambodia. The findings of our study would be useful for assessment of host/vector interaction and instruct diagnosis of tick-borne pathogens.

项目总结 背景：在柬埔寨，扁虱和硬虱传播的疾病被忽视，数据仍然缺乏。目标： 在目前的建议中，我们的目标是识别扁虱的种类和相关的病原体，包括病毒和细菌。 在柬埔寨农村地区流传。此外，我们计划调查一些选定的扁虱的传播风险- 通过评估血清抗体(IgG)和扁虱叮咬已知在人类该地区传播的病原体 生活在扁虱捕获社区的人们中的生物标记物。方法：我们的研究将在 一项研究，即“柬埔寨磅通省登革热类疾病研究”(DENTHOM)，是 由NIH/NIAID资助的一个项目，即“关于流行性感冒的传播、适应和发病机制的区域间研究” 东南亚和西非/中非具有大流行潜力的病毒“(U01 AI151758-01)。Tick集合 将根据家长研究(DENTHOM)进行规划。在人体样本方面，本研究 仅涉及在DENTHOM研究框架内收集的生物胺。没有收集人类生物 将为本提案专门制作样本。收集到的扁虱标本将是第一个 形态鉴定和MALDI-TOF MS确认一旦鉴定，标本将被杀死并 扁虱样本将保持在零下80摄氏度，使用分子检测方法检测已知病毒和细菌，并检测新的病毒和细菌 病原体的元基因组测序。人体样本将用于一般情况下的血清阳性率研究 已知的扁虱传播病毒和细菌在亚洲流行，并用于扁虱叮咬生物标记物。预期结果：我们的 这项研究将提供有关扁虱的知识现状和与扁虱相关的病原体传播的潜在风险 在柬埔寨。我们的研究结果将有助于评估宿主/载体的相互作用并指导 壁虱传播病原体的诊断。