Harnessing single cell epigenome-wide profiling of myeloid cells to compare and contrast Alzheimer's from HIV-associated cognitive dysfunction

项目摘要/摘要(适用于Alina Pang(Ndhlovu)：R01 AG063846-01A1 补充申请) 在这项申请中，我们寻求一个多样性研究的补充，以家长奖 比较和对比阿尔茨海默病的髓系细胞的表观基因组图谱 与艾滋病毒相关的认知功能障碍“(AG063846-01A1)。家长奖旨在 确定髓系人群的表观基因组图谱，以区分与HIV相关的 阿尔茨海默病轻度认知障碍状态的神经认知性疾病 艾滋病毒携带者接受抑制性抗逆转录病毒治疗(ART)。这一多样性补充将扩大 具体目标1的研究范围包括无细胞DNA(CfDNA)的表观遗传特征，以进一步帮助 为了阐明艾滋病毒携带者(PLWH)和 提供手部发病的表观遗传学和免疫学方面的博士后培训。的目标是 多样性补充是为了让彭博士获得关于表观遗传学特征的经验和初步数据 针对神经认知障碍的周边地区在2023年提交K01申请。

PROJECT SUMMARY/ ABSTRACT (for Alina Pang (Ndhlovu): R01 AG063846-01A1 Supplement Application) In this application, we seek a diversity research supplement to parent award “HARNESSING SINGLE CELL EPIGENOME-WIDE PROFILING OF MYELOID CELLS TO COMPARE AND CONTRAST ALZHEIMER'S FROM HIV-ASSOCIATED COGNITIVE DYSFUNCTION” (AG063846-01A1). The parent award seeks to define the epigenomic landscape of myeloid populations in order to differentiate HIV Associated Neurocogntivie Disease (HAND) from Mild Cognitive Impairment state of Alzheimer’s Disease (MCI-AD) in individuals living with HIV on suppressive antiretroviral therapy (ART). This diversity supplement will expand the scope of research in Specific Aim 1 to include epigenetic signatures of cell free DNA (cfDNA) to further aid in elucidating the pathogenesis of neurocognitive impairment observed in people living with HIV (PLWH) and provide postdoctoral training in epigenetics and immunology in the pathogenesis of HAND. The goal of the diversity supplement is for Dr. Pang to gain experience and preliminary data on epigenetic signatures in the periphery specific to neurocognitive impairment to submit a K01 application in 2023.