Harnessing single cell epigenome-wide profiling of myeloid cells to compare and contrast Alzheimer's from HIV-associated cognitive dysfunction

利用骨髓细胞的单细胞表观基因组分析来比较和对比阿尔茨海默病与艾滋病毒相关的认知功能障碍

• 批准号: 10632875
• 负责人: Lishomwa C Ndhlovu
• 金额: $ 6.3万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-15 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10632875
• 关键词: Alzheimer' s Disease; Award; Blood; Cells; Data; Diagnosis; Disease; Epigenetic Process; Goals; HIV; HIV-associated cognitive impairment; Immune; Immunology; Individual; Myelogenous; Myeloid Cells; Neurocognitive Deficit; Parents; Pathogenesis; Patients; Persons; Population; Research; antiretroviral therapy; brain dysfunction; cell free DNA; circulating DNA; epigenome; epigenomics; experience; mild cognitive impairment; post-doctoral training; public health relevance

PROJECT SUMMARY/ ABSTRACT (for Alina Pang (Ndhlovu): R01 AG063846-01A1 Supplement Application) In this application, we seek a diversity research supplement to parent award “HARNESSING SINGLE CELL EPIGENOME-WIDE PROFILING OF MYELOID CELLS TO COMPARE AND CONTRAST ALZHEIMER'S FROM HIV-ASSOCIATED COGNITIVE DYSFUNCTION” (AG063846-01A1). The parent award seeks to define the epigenomic landscape of myeloid populations in order to differentiate HIV Associated Neurocogntivie Disease (HAND) from Mild Cognitive Impairment state of Alzheimer’s Disease (MCI-AD) in individuals living with HIV on suppressive antiretroviral therapy (ART). This diversity supplement will expand the scope of research in Specific Aim 1 to include epigenetic signatures of cell free DNA (cfDNA) to further aid in elucidating the pathogenesis of neurocognitive impairment observed in people living with HIV (PLWH) and provide postdoctoral training in epigenetics and immunology in the pathogenesis of HAND. The goal of the diversity supplement is for Dr. Pang to gain experience and preliminary data on epigenetic signatures in the periphery specific to neurocognitive impairment to submit a K01 application in 2023.

项目总结/摘要（针对Alina Pang（Ndhlovu）：R 01 AG 063846 - 01 A1 补充申请） 在这个应用程序中，我们寻求一个多样性研究补充家长奖“HARNESSING单细胞 用全表位基因组分析比较和预测阿尔茨海默病 HIV相关的认知功能障碍”（AG 063846 - 01 A1）。家长奖旨在 定义髓系人群的表观基因组景观，以区分HIV相关 阿尔茨海默病的轻度认知损害状态（MCI-AD）中的神经认知疾病（HAND） 接受抑制性抗逆转录病毒治疗（ART）的艾滋病毒感染者。这种多样性补充将扩大 具体目标1的研究范围包括无细胞DNA（cfDNA）的表观遗传特征，以进一步帮助 阐明在艾滋病毒感染者（PLWH）中观察到的神经认知障碍的发病机制， 提供HAND发病机制的表观遗传学和免疫学博士后培训。的目标 多样性补充是为了让彭博士获得经验和初步数据的表观遗传签名在 针对神经认知障碍的外围设备在2023年提交K 01申请。