Quantitative Immunological Assays to Assess HIV Antibodies and Vaccines

评估 HIV 抗体和疫苗的定量免疫分析

• 批准号: 10928625
• 负责人: Nicole Doria-Rose
• 金额: $ 89.24万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10928625
• 关键词: Animals; Antibodies; Biological Assay; Clinical Trials; Data; Data Analyses; Genes; HIV; HIV Antibodies; HIV vaccine; HIV-1; Immune response; Immunology procedure; Individual; Industry Collaboration; Infection; Infrastructure; Liquid substance; National Institute of Allergy and Infectious Disease; Persons; Protocols documentation; Reagent; Robotics; Role; Running; Sampling; Serum; Services; Standardization; Vaccinated; Vaccine Research; Vaccines; Variant; Virus; Work; antibody engineering; antibody test; candidate selection; clinical candidate; improved; manufacturability; mutant; neutralizing antibody

We are a Core lab providing HIV antibody assay services to labs within the Vaccine Research Center, other intramural labs, and for collaborators around the world. Most of our work uses a standardized HIV-1 neutralization assay. We perform this assay on serum samples from vaccinated animals and HIV-infected individuals, on antibodies isolated from such subjects, and on variant antibodies engineered for improved breadth, potency, and manufacturability. We also develop and maintain the reagents needed for these assays, including natural and mutant virus genes, stocks of viruses, and stocks of reference antibodies. We recently have increased the throughput of our assay by integrating robotic liquid handling into our protocols. In addition, we provide analysis and graphics to allow interpretation of our data, as well as related data from the VRC VIP lab; these are being used for selection of candidate clinical products at the VRC and by collaborators. We also provide data to collaborators at a pharma company to inform their decisions on developing products for clinical trials.

我们是一个核心实验室，为疫苗研究中心内的实验室、其他校内实验室以及世界各地的合作者提供HIV抗体检测服务。我们的大部分工作使用标准化的HIV-1中和试验。我们对来自接种疫苗的动物和HIV感染者的血清样品、从这些受试者中分离的抗体以及为改善宽度、效力和可制造性而设计的变体抗体进行了该测定。我们还开发和维护这些检测所需的试剂，包括天然和突变病毒基因、病毒储备和参考抗体储备。我们最近通过将机器人液体处理集成到我们的方案中来提高我们的测定的通量。此外，我们还提供分析和图形，以解释我们的数据以及来自VRC VIP实验室的相关数据;这些数据正在VRC和合作者用于选择候选临床产品。我们还为制药公司的合作者提供数据，以帮助他们做出开发临床试验产品的决策。