REGULATION OF PREMEIOTIC DNA REPLICATION IN S POMBE

描述(改编自研究人员摘要)：长期目标 这项研究的目的是确定真核细胞如何调控这一过程 减数分裂前DNA复制，并将其与减数分裂中的其他事件耦合。 如果不能适当地调节减数分裂，可能会导致不适当的分离 遗传物质，以及无法存活或受损的配子，这可能会导致出生 缺陷。这项工作利用了一种简单的真核生物的遗传学， 裂殖酵母，裂殖酵母。从以下方面获得的见解 从简单的遗传系统研究细胞现象有助于 描述在包括人类在内的复杂生物体中发生的事件，以及 因为减数分裂的过程是所有性发育的共同过程 对于真核生物来说，许多元素很有可能是保守的。 该提案的具体目标如下。第一，程度 有丝分裂和减数分裂DNA复制调控之间的重叠将是 评估过了。已知的营养细胞S阶段所需的基因，或 为了将复制与其他细胞周期事件相结合，将分析 它们在减数分裂中的作用。减数分裂细胞对DNA损伤的反应将是 已确定，以及对已确定的复制来源的要求 减数分裂前的DNA合成将被检测。这将提供部分 S减数分裂时期的事件描述及其分析工具 再远一点。第二，影响减数分裂前DNA的新基因 复制，或减数分裂前DNA复制到下游的耦合 减数分裂事件将被隔离。最后，分子、生化和 将对已确定的新基因座进行遗传分析，以确定 维持事件秩序的跨行为监管机构网络 减数分裂。这将允许对监管的详细描述 真核生物减数分裂前DNA复制模型。

DESCRIPTION (adapted from investigator's abstract): The long term objective of this research is to determine how eukaryotic cells regulate the process of premeiotic DNA replication and couple it to other events in meiosis. Failure to properly regulate meiosis can lead to improper segregation of the genetic material, and inviable or damaged gametes, which can result in birth defects. This work exploits the genetics of a simple eukaryotic organism, the fission yeast, Schizosaccharomyces pombe. The insights gained from studying cellular phenomena from simple genetic systems are helpful in describing the events occurring in complex organisms, including man, and because the process of meiosis is common to all sexually developing eukaryotes, there is a good chance that many elements will be conserved. The specific aims of the proposal are as follows. First, the degree of overlap between regulation of mitotic and meiotic DNA replication will be assessed. Genes known to be required for S phase in vegetative cells, or for coupling replication to other cell cycle events, will be analyzed for their role in meiosis. The response of meiotic cells to DNA damage will be determined, and the requirement for identified origins of replication in premeiotic DNA synthesis will be assayed. This will provide a partial description of the events of meiotic S phase as well as tools to analyze it further. Second, new genes specifically affecting premeiotic DNA replication, or the coupling of premeiotic DNA replication to downstream meiotic events will be isolated. Finally, molecular, biochemical, and genetic analysis will be carried out on the new loci identified to establish the network of trans-acting regulators that preserves the order of events in meiosis. This will allow a thorough description of the regulation of premeiotic DNA replication in a model eukaryote.