HOMEOBOX GENE VOX AND DEVELOPMENT

同源盒基因 VOX 和发育

• 批准号: 2418042
• 负责人: ANNA E MELBY
• 金额: $ 2.54万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-08 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2418042
• 关键词: Xenopus; developmental genetics; homeobox genes

The vertebrate embryo is patterned by opposing signals that originate both dorsally and ventrally in the gastrula. This proposal examines the role of Vox, a Xenopus homeobox gene that is positively regulated by the ventral signaling molecule BMP-4. Overexpression of Vox causes embryos to become ventralized, and suppresses dorsal gene expression. Vox is expressed in a similar pattern to BMP-4, and therefore it might be a downstream mediator of BMP-4. However, Vox overexpression causes increased BMP-4 levels. Thus, Vox might act to stabilize BMP-4 expression via an autoregulatory loop, without activating genes that are targets of BMP-4. My first aim is to understand how Vox fits in the regulatory hierarchy of ventral specification. To determine whether Vox acts downstream of BMP-4, I will see if injected Vox RNA can activate targets of BMP-4 in embryos where BMP-4 signaling is blocked by overexpression of a dominant-negative BMP receptor. My second aim is to determine the requirements for Vox during development. I will examine how development and gene expression patterns are affected in embryos injected with a dominant negative Vox mutant.

脊椎动物的胚胎是由相反的信号， 在原肠胚的背侧和腹侧。 本提案审查了 Vox的作用，非洲爪蟾同源框基因，正调控 腹侧信号分子BMP-4 Vox的过度表达导致 胚胎变得腹侧化，并抑制背侧基因表达。 Vox的表达模式与BMP-4相似，因此它可能 是BMP-4的下游介质。 然而，Vox过度表达 导致BMP-4水平升高。 因此，Vox可能起到稳定BMP-4的作用， 通过自动调节环表达，而不激活 BMP-4的目标。 我的第一个目标是了解Vox如何适应监管层级 腹部规格。 为了确定Vox是否在 BMP-4，我将看看注射的Vox RNA是否能激活BMP-4的靶点， BMP-4信号传导被过表达的 显性负性BMP受体。 我的第二个目标是确定 在开发过程中对Vox的要求。 我将研究如何 胚胎的发育和基因表达模式受到影响 注射了一种显性负性Vox突变体