NOREPINEPHRINE ANDROGEN INTERACTIONS AND VOCAL BEHAVIOR

去甲肾上腺素雄激素相互作用和声音行为

• 批准号: 2591681
• 负责人: LIN M CHAVES
• 金额: $ 2.21万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-16 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2591681
• 关键词: Aves; animal communication behavior; behavioral /social science research tag; experimental brain lesion; high performance liquid chromatography; immunocytochemistry; neural plasticity; neuroendocrine system; norepinephrine; photostimulus; testosterone; vocalization

The proposed study will investigate the noradrenergic system and its influence on the action of testosterone in relation to vocal behavior in a songbird species the European starling (Sturnus vulgaris). First, sources of noradrenergic input to telencephalic areas of the starling vocal control system will be determined through use of tract- tracing techniques in combination with immunocytochemical markers of catecholaminergic activity (dopamine beta hydroxylase [DBH] and tyrosine hydroxylase [TH]). Identified sources of noradrenergic innervation will then be damaged with electrolytic lesions and the consequences of disrupting this input on monoamines levels in the telencephalic song nuclei will be assessed by high performance liquid chromatography (HPLC). The second portion of this study will address the role of afferent input from norepinephrine (NE) in maintaining hormone-induced plasticity in adulthood. This will be addressed in two experimental contexts; 1) photoperiod-induced seasonal changes in volume of the hyperstriatum ventrale pars caudale (HVC) of male and female starlings, 2) the production of "male-like" song in female starlings and accompanying volume changes in HVC after T implants. By evaluating the effects of testosterone manipulations on adult song behavior and HVC volume after the use of electrolytic lesions to remove sources of noradrenergic input to song nuclei, new insights into the role of the noradrenergic system in maintaining testosterone-dependent neural and behavioral responses can be gained.

这项研究将探讨去甲肾上腺素能系统及其在脑内的作用。 影响睾丸激素的行动与声乐行为在一个 鸣禽欧洲椋鸟（Sturnus vulgaris）。首先，去甲肾上腺素能输入端脑区的来源， 椋鸟的发声控制系统将通过使用声道来确定， 示踪技术结合免疫细胞化学标记 儿茶酚胺能活性（多巴胺β羟化酶[DBH]和酪氨酸 羟化酶[TH]）。已确定的去甲肾上腺素能神经支配来源将 然后被电解损伤所破坏， 干扰端脑鸣唱中单胺水平的输入 通过高效液相色谱法（HPLC）评估细胞核。 本研究的第二部分将讨论传入输入的作用 从去甲肾上腺素（NE）在维持神经元诱导的可塑性， 成年这将在两个实验环境中解决; 1） 光周期引起的上纹状体体积的季节性变化 雄性和雌性椋鸟腹侧尾侧部（HVC）; 2） 雌椋鸟“雄性样”鸣叫的产生及其伴随音量 T植入后HVC的变化。通过评估睾丸激素的作用 使用后对成人歌曲行为和HVC音量的操作 电解损伤以去除对鸣唱的去甲肾上腺素能输入源 核，新的见解去甲肾上腺素能系统的作用， 维持睾丸激素依赖的神经和行为反应， 获得。