REGULATION OF PLACENTAL DEVELOPMENT BY ESTROGEN

目前的研究将检验这一假设，即雌激素对 在规划扩散和/或形态方面的作用 细胞滋养层细胞分化与胎盘生长 通过发育调节来怀孕，在一个 自分泌/旁分泌方式，表达和产生IGF- LI/IGFBP-2、EGF/EGF受体。滋养层细胞的个体发育调控 增殖/形态分化与生长因子 将确定在从狒狒身上获得的胎盘中的表达 妊娠45、60和100天(足月=184天)。的作用 同样参数的雌激素将在获得的胎盘中进行研究。 从雌激素水平过早升高的狒狒身上 在怀孕早期。雌激素对肽生长的时间效应 因子表达与滋养层细胞增殖/形态 将在短期研究(24小时和48小时)中确定差异 在怀孕第45天向狒狒注射雄烯二酮后)。 用增殖细胞核抗原(DNA指数)评价滋养层细胞的增殖 复制)，形态分化将通过以下方式评估 组织学和CS产生。MRNAs的定量和定位 以及IGF-II/IGFBP-2、EGF/EGF受体的蛋白质将被测定 通过RT-PCR/Northern/Western印迹和原位杂交， 分别进行了分析。这项拟议的研究有望提供新的见解 胎盘生长发育调控的研究进展 灵长类动物妊娠早期的分化。

The present study will test the hypothesis that estrogen has a major role in programming the proliferation and/or morphological differentiation of cytotrophoblasts and growth of the placenta during baboon pregnancy through developmental regulation, in an autocrine/paracrine manner, of expression and production of IGF- lI/IGFBP-2, EGF/EGF receptor. Ontogenetic regulation of trophoblast proliferation/ morphological differentiation and growth factor expression will be determined in placentas obtained from baboons on days 45, 60 and 100 of gestation (term = 184 days). The role of estrogen on the same parameters will be studied in placentas obtained from baboons in which estrogen levels have been prematurely elevated early in pregnancy. The temporal effect of estrogen on peptide growth factor expression and trophoblast proliferation/morphological differentiation will be determined in short-term studies (24 and 48 h after injection of androstenedione to baboons on day 45 of pregnancy). Trophoblast proliferation will be assessed by PCNA (index of DNA replication), and morphological differentiation will be assessed by histology and CS production. Quantification and localization of mRNAs and proteins for IGF-II/IGFBP-2, EGF/EGF receptor will be determined by RT-PCR/Northern/Western blot and in situ hybridization, respectively. The proposed study is expected to provide new insight into the developmental regulation of placental growth and differentiation during early primate pregnancy.