CORE--PREPARATION, SUPPLY OF TOXOPLASMA AND IMMUNOLOGICALS

在本资助提案中，各个项目的设计目的是 研究新的抗T.弓形虫， 研究新的酶途径，可能对设计 对寄生虫有效的药物，并确定和表征 负责抗药性的基因。 的 最终目标是确定更有效和更安全的治疗方法， 用于治疗弓形虫病，特别是弓形虫病的药物 艾滋病患者的脑炎。 对于每一项拟议的研究， 速殖子、包囊和缓殖子。刚地会 需要大量的。 速殖子和包囊将用于 体内组织培养中小鼠和哺乳动物细胞感染 和体外评价治疗剂对T. 刚地。 速殖子和缓殖子也将用于研究 生物化学和代谢途径，这可能被证明是有用的， 治疗剂灭活或抑制的靶点。 这些 T的形式。将由该核心提供弓形虫。 此外，该核心 也将提供T.用于血清学和 用于制备IFN-γ免疫化学测定和抗血清 T.弓形虫对小鼠的低毒力。

In the present grant proposal the individual projects are designed to examine the activity of new therapeutic agents against T. gondii, to investigate new enzymatic pathways that may prove important for design of drugs active against the parasite and to identify and characterize the genes of the organism that are responsible for drug resistance. The ultimate objectives are to identify more active and safer therapeutic agents for treatment of toxoplasmosis, particularly toxoplasmic encephalitis in AIDS patients. For each of the proposed studies, tachyzoites, cysts and bradyzoites of different strains of T. gondii will be needed in large numbers. Tachyzoites and cysts will be used for infection of mice and of mammalian cells in tissue culture for in vivo and in vitro evaluation of the activity of therapeutic agents against T. gondii. Tachyzoite and bradyzoites will also be used to investigate biochemical and metabolic pathways which may prove to be useful as targets for inactivation or inhibition by therapeutic agents. These forms of T. gondii will be provided by this Core. In addition, this Core will also provide antisera to T. gondii for use in serological and immunochemical assays and antisera to IFN-gamma for use un preparation of tachyzoites of strains of T. gondii of low virulence for mice.