CORE--PREPARATION, SUPPLY OF TOXOPLASMA AND IMMUNOLOGICALS

在目前的赠款提案中，个别项目的设计目的是 检测新的治疗药物对弓形虫的活性， 研究可能被证明对设计重要的新的酶途径 有效对抗寄生虫的药物，并鉴定和表征 导致抗药性的生物体基因。这个 最终目标是找到更积极和更安全的治疗方法 治疗弓形虫病，特别是弓形虫病的药物 艾滋病患者的脑炎。对于每一项拟议的研究， 不同株弓形虫的速殖子、包囊和缓殖子 需要大量的资金。速殖子和包囊将用于 体内组织培养中小鼠和哺乳动物细胞的感染 体外评价治疗药物对T。 贡地。速殖子和缓殖子也将被用来调查 生化和代谢途径，可能被证明是有用的 治疗药物失活或抑制的靶标。这些 弓形虫的形式将由该中心提供。另外，这个核心 还将提供弓形虫的抗血清，用于血清学和 制备用干扰素-γ免疫化学检测和抗血清 对小鼠低毒力弓形虫株的速殖子。

In the present grant proposal the individual projects are designed to examine the activity of new therapeutic agents against T. gondii, to investigate new enzymatic pathways that may prove important for design of drugs active against the parasite and to identify and characterize the genes of the organism that are responsible for drug resistance. The ultimate objectives are to identify more active and safer therapeutic agents for treatment of toxoplasmosis, particularly toxoplasmic encephalitis in AIDS patients. For each of the proposed studies, tachyzoites, cysts and bradyzoites of different strains of T. gondii will be needed in large numbers. Tachyzoites and cysts will be used for infection of mice and of mammalian cells in tissue culture for in vivo and in vitro evaluation of the activity of therapeutic agents against T. gondii. Tachyzoite and bradyzoites will also be used to investigate biochemical and metabolic pathways which may prove to be useful as targets for inactivation or inhibition by therapeutic agents. These forms of T. gondii will be provided by this Core. In addition, this Core will also provide antisera to T. gondii for use in serological and immunochemical assays and antisera to IFN-gamma for use un preparation of tachyzoites of strains of T. gondii of low virulence for mice.