BLR&D Research Career Scientist Award Application

这份申请是为了竞争性地续签普拉迪普·K·杜德贾博士的SRCS计划。他的计划是 侧重于阐明人体肠道电解质和液体吸收的调节机制 在健康和疾病方面。他的研究旨在了解腹泻疾病的病理生理学。 属于感染性腹泻和炎症性肠病(IBD)相关性腹泻 更好的治疗干预。腹泻病在我们的退伍军人中非常常见，因为 IBD、感染和部署相关的慢性病和压力。我们的节目使用了最先进的技术 途径包括人肠道或迷你肠道和肠样来源的单层，器官供体 人肠道不同部位纯化的反足质膜的体外研究 利用基因敲除和转基因小鼠模型的分子方法和体内方法 了解腹泻的病理生理学，并确定新的治疗靶点。我们的团队已经有了一个 由于我们建立的一些开创性技术，在全球领先该领域的独特优势 利用器官捐赠者的人体肠道。我们已经在220多个原创同行上广泛发表了我们的工作 回顾了该领域许多著名期刊上的研究论文，包括胃肠病学(影响因素 21)和J Clin Invest(12.3)。我们还广泛定义了肠道-微生物相互作用在腹泻中的作用。 与感染性腹泻有关的疾病以及益生菌在这些疾病中的有益作用。此外, 我们项目的主要标志之一是研究的多学科和协作性 专注于指导新一代退伍军人和附属大学调查人员的企业 使他们能够建立自己的独立研究计划。我们在这两个方面都取得了成功 在该领域取得新发现并指导新一代年轻科学家和 在我们当地的退伍军人事务部和附属大学建立一个坚实的研究环境。我们的节目有 由退伍军人事务部和美国国立卫生研究院颁发的多个奖项持续资助。此当前应用程序旨在突出显示 我们的计划取得成功，并请求续签此SRCS计划，以继续朝着 通过培养和指导新一代调查人员达到成功的新高度。

This application is for a competitive renewal of the SRCS program of Dr. Pradeep K Dudeja. His program is focused on elucidating the mechanisms of regulation of electrolyte and fluid absorption in the human intestine in health and disease. His studies are aimed at understanding the pathophysiology of diarrheal diseases as it pertains to infectious diarrhea and inflammatory bowel diseases (IBD) associated diarrhea and to develop better therapeutic interventions. Diarrheal diseases are very common in our Veterans due to high incidence of IBD, infections and deployment related chronic illnesses and stress. Our program utilizes state-of-the-art approaches including human intestinal enteroids or mini guts and enteroid derived monolayers, organ-donor human intestine derived purified antipodal plasma membranes from various regions of the gut, in vitro molecular approaches and in vivo approaches utilizing knock out and transgenic mouse models to better understand the pathophysiology of diarrhea and to identify novel therapeutic targets. Our group has had a unique advantage of leading this field globally due to some of the pioneering techniques established by us using the organ donor human intestines. We have extensively published our work in over 220 original peer reviewed research articles in many prestigious journals in the field including Gastroenterology (impact factor 21) and J Clin Invest (12.3). We have also extensively defined the role of gut-microbe interactions in diarrheal diseases with respect to infectious diarrhea and the beneficial role of probiotics in these diseases. In addition, one of the key hallmarks of our program has been a multidisciplinary and collaborative nature of the research enterprise with a deep focus on mentoring new generation of VA and affiliated university investigators to enable them to establish their own independent research programs. We have been successful at both the fronts of making new discoveries in the field and mentoring new generation of young scientists and establishing a solid research environment at our local VA as well as at affiliated university. Our program has been continuously funded by multiple VA and NIH awards. This current application is intended to highlight the success of our program and requesting renewal of this SRCS program to continue the progress towards the new heights of success by nurturing and mentoring a new generation of investigators.