Flow Cytometry and Confocal Microscopy Shared Resource

流式细胞术和共焦显微镜共享资源

• 批准号: 10625766
• 负责人: Patrick Kellish
• 金额: $ 7.83万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10625766
• 关键词: Abbreviations; Accounting; Adherence; Antibodies; Applications Grants; Back; Biological Markers; Biometry; Biotechnology; Brain; COVID-19 pandemic; Cancer Center; Cancer Center Support Grant; Cancer Control; Cell Separation; Cells; Cellular Morphology; Certification; Charge; Clinical; Collaborations; Communities; Complex; Confocal Microscopy; Consultations; Data; Data Analyses; Development; Doctor of Philosophy; Educational workshop; Effectiveness; Ensure; Equipment; Experimental Designs; Feedback; Florida; Flow Cytometry; Funding; Future; Genetic Research; Growth; Health; Hour; Image; Immune; Immune response; Immunobiology; Immunologist; Immunophenotyping; Immunotherapy; Infrastructure; Institution; Knowledge; Laboratories; Lasers; Last Name; Letters; Malignant Neoplasms; Methods; Microscopy; Mission; Modernization; NCI Center for Cancer Research; Peer Review; Phenotype; Policies; Population Sciences; Postdoctoral Fellow; Procedures; Productivity; Publications; RNA vaccine; Recommendation; Reproducibility; Research; Research Activity; Research Project Grants; Research Support; Reservations; Resource Sharing; Resources; Sampling; Scanning; Science; Services; Solid Neoplasm; Sorting; Strategic Planning; Students; Surface; Surface Antigens; Surveys; System; Technology; Tissues; Training; United States National Institutes of Health; Universities; Vaccine Clinical Trial; Vendor; anticancer research; cost; cost effective; design; exosome; experience; first-in-human; fluorescence microscope; improved; instrument; instrumentation; live cell imaging; member; multi-photon; operation; particle; programs; recruit; response; satisfaction; single cell analysis; student training; time use; treatment response; tumorigenesis; ultra high resolution

FLOW CYTOMETRY AND CONFOCAL MICROSCOPY SHARED RESOURCE (CYT-SR): ABSTRACT CYT-SR provides University of Florida Health Cancer Center (UFHCC) members with cost-effective, state-of- the-art flow cytometry and modern confocal microscopy expertise, instrumentation, and services to support their research activities. The CYT-SR is an institutionally-managed shared resource (SR) that is supported by UFHCC through a partnership with the UF Interdisciplinary Center for Biotechnology Research (ICBR). It is co-located within the Cancer & Genetics Research Complex and McKnight Brain Institute, providing convenient access for UFHCC members. The CYT-SR has 4 staff with over 22 years combined experience including Kellish, the Scientific Director, an immunologist with more than 7 years of experience in all aspects of flow analysis. Key technologies available in the CYT-SR include multi-parameter flow cytometry, cell sorting, live cell imaging via confocal microscopy, surface biomarker-based cancer and immune cell phenotyping, assessment of tissue and cell morphology by confocal microscopy, and small particle and exosome analysis. During the recent COVID-19 pandemic, the CYT-SR was identified as an essential campus resource and remained open and accessible to all users. Instrument reservations, equipment usage time, and technician-assisted activities are tracked by the UFHCC and charged back via the CrossLab laboratory management system. Since 2016, the CYT-SR was utilized by 78 unique UFHCC users (43 with peer-reviewed funding) accounting for ~24% of all users of the facility. CYT-SR use by UFHCC members increased 5.3-fold from 900 hours in 2016 to 4,800 hours in 2021. The CYT-SR supported more than 180 publications, with 24% having IF >10. Since the 2019 CCSG submission, 484 students, postdoctoral fellows, and technical staff from UFHCC member laboratories were trained on CYT- SR instruments. Mechanisms of Oncogenesis and the Cancer Therapeutics and Host Response members represent the majority of UFHCC users. The effectiveness of the CYT-SR in enhancing member research productivity is assessed by annual surveys, feedback from the CYT-SR Scientific Advisory Group, and recommendations from the SR Oversight Committee. Due to the rising demand for CYT-SR resources by members, the UFHCC supported the purchase of a new Sony SP6800 Spectral Analyzer (2018), Cytoflex LX21 (2020), and a Becton Dickinson SYMPHONY A3 (2020). To support first-in-human RNA vaccine clinical trials, UFHCC supported the recruitment of a PhD-level immunologist to the CYT-SR to perform flow-based immunophenotyping. Future growth of the CYT-SR are in alignment with the UFHCC strategic plan, Momentum 2027, and user satisfaction surveys, and include upgrades such as an acquisition of a BD FACSYMPHONY S6 cell sorter, increasing capabilities and services in super high-resolution microscopy and single cell analysis, and in collaboration with BQS-SR, expansion of advanced training in the application of biostatistics to cytometric analysis.

流式细胞术和共聚焦显微镜共享资源(CyT-SR)：摘要 CyT-SR为佛罗里达大学健康癌症中心(UFHCC)成员提供经济高效、最先进的 最先进的流式细胞术和现代共聚焦显微镜的专业知识、仪器和服务，以支持其 研究活动。CyT-SR是由UFCC支持的制度性管理的共享资源(SR 通过与密歇根大学生物技术研究跨学科中心(ICBR)建立合作伙伴关系。它位于同一地点 在癌症和遗传学研究综合体和麦克奈特脑研究所内，为 UFCC会员。CyT-SR有4名员工，他们总共有22年的工作经验，其中包括Kellish， 科学总监，一位免疫学家，在流动分析的各个方面都有超过7年的经验。钥匙 CyT-SR中可用的技术包括多参数流式细胞术、细胞分选、活细胞成像 共聚焦显微镜，基于表面生物标记物的癌症和免疫细胞表型，组织和 共聚焦显微镜观察细胞形态，小颗粒和外切体分析。在最近的新冠肺炎期间 在大流行期间，CyT-SR被确定为基本的校园资源，并保持开放和可访问 所有用户。仪器预订、设备使用时间和技术人员协助的活动由 并通过CrossLab实验室管理系统进行计费。自2016年以来，CyT-SR是 由78个独立的UFCC用户(43个拥有同行审查资金的用户)使用，约占所有用户的24% 设施。UFCC成员使用细胞色素T-SR的时间从2016年的900小时增加到2021年的4800小时，增加了5.3倍。 CyT-SR支持了180多种出版物，其中24%拥有IF&>10。自2019年CCSG提交以来， 来自UFCC成员实验室的484名学生、博士后研究员和技术人员接受了关于细胞毒性的培训- 高级仪器。肿瘤发生机制与肿瘤治疗和宿主反应成员 代表UFCC的大多数用户。细胞色素T-SR在加强会员研究中的有效性 生产力是通过年度调查、来自CyT-SR科学咨询小组的反馈来评估的，以及 SR监督委员会的建议。由于对CyT-SR资源的需求不断上升， 成员们，UFCC支持购买新的索尼SP6800频谱分析仪(2018)，Cytoflex LX21 (2020)，以及贝顿·狄金森交响曲A3(2020)。为了支持首个人类RNA疫苗的临床试验， UFCC支持招聘一名博士级别的免疫学家到细胞色素T-SR进行基于流量的研究 免疫表型。CyT-SR的未来增长与UFCC的战略计划、势头一致 和用户满意度调查，并包括升级，如收购BD FACSYMPHONY S6 细胞分选机，提高超高分辨率显微镜和单细胞分析的能力和服务，以及 与BQS-SR合作，扩大将生物统计学应用于细胞学的高级培训 分析。